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微流体注入-热透镜显微系统高灵敏的快速检测微囊藻毒素

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分析科学学报 2019年第1期35卷 110-114页

作者：刘明强 franko mladen 西南科技大学理学院四川绵阳621010 新戈里察大学环境与生命科学实验室

基于蛋白磷酸酶抑制反应,发展了微流体注入-热透镜显微探测系统,并用于高灵敏的快速检测水体中微囊藻毒素。研究了反应时间、底物浓度和酶活性等因素对检测微囊藻毒素的影响;给出了最优的反应参数。与传统基于微量滴定板的微囊藻毒素检... 详细信息

基于蛋白磷酸酶抑制反应,发展了微流体注入-热透镜显微探测系统,并用于高灵敏的快速检测水体中微囊藻毒素。研究了反应时间、底物浓度和酶活性等因素对检测微囊藻毒素的影响;给出了最优的反应参数。与传统基于微量滴定板的微囊藻毒素检测方法相比,酶的用量可减少一个数量级,并且将分析时间从30min缩短至6min。该检测方法对微囊藻毒素LR的检出限约为20ng/L。测试了来自一渔场的水样,发现水体中微囊藻毒素水平低于本方法的检出限。

关键词：微流体注入分析热透镜显微技术微囊藻毒素蛋白磷酸酶抑制反应

Intra-and interobserver reliability of ocular surface analyzer LacryDiag

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International Journal of Ophthalmology(English edition) 2024年第8期17卷 1418-1422页

作者： Eva Kos Valentina Cigić mladen Bušić Mirjana Bjeloš Daliborka Miletić Biljana KuzmanovićElabjer University Eye Clinic University Hospital“Sveti Duh”Sveti Duh 64Zagreb 10000Croatia Faculty of Dental Medicine and Health Care Osijek Faculty of Medicine OsijekUniversity Josip Juraj Strossmayer in OsijekOsijek 31000Croatia

AIM:To invastigate intra-and interobserver reliability of interferometry,tear meniscus height(TMH)measurement and meibography(MBG)of an ocular surface analyzer,LacryDiag(Quantel Medical,France).METHODS:Five consecutive measurements and subsequent analysis of interferometry,TMH,and MBG were recorded by two examiners using the *** assess intra-and interobserver reliability,we used Cohen’s kappa for categorical variables(interferometry),or intraclass correlation coefficient for continuous variables(TMH,MBG).RESULTS:Thirty eyes of 30 examinees were *** both observers,there was excellent intraobserver reliability for MBG(0.955 and 0.970 for observer 1 and 2,respectively).Intraobserver reliability for observer 1 was substantial for interferometry(0.799),and excellent for TMH(0.863).Reliability for observer 2 was moderate for interferometry(0.535)and fair to good for TMH(0.431).Interobserver reliability was poor for interferometry(0.074)and fair to good for TMH(0.680)and MBG(0.414).CONCLUSION:LacryDiag ocular surface analyzer in our study proves to be a reliable noninvasive tool for the evaluation of TMH and *** for interferometry,poor interobserver reliability,fair to good intraobserver reliability for observer 1,and moderate for observer 2,leave room for improvement.

关键词： dry eye ocular surface analyzer interferometry tear meniscus height meibography

Decoding Retinoblastoma: Differential Gene Expression

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International Journal of Clinical Medicine 2024年第4期15卷 177-196页

作者： Ahmed Jasim Mahmood Al-Mashhadani franko Shehaj Lianhong Zhou Department of Ophthalmology Renmin Hospital of Wuhan University Wuhan China

Background: Retinoblastoma, the most common intraocular pediatric cancer, presents complexities in its genetic landscape that necessitate a deeper understanding for improved therapeutic interventions. This study leverages computational tools to dissect the differential gene expression profiles in retinoblastoma. Methods: Employing an in silico approach, we analyzed gene expression data from public repositories by applying rigorous statistical models, including limma and de seq 2, for identifying differentially expressed genes DEGs. Our findings were validated through cross-referencing with independent datasets and existing literature. We further employed functional annotation and pathway analysis to elucidate the biological significance of these DEGs. Results: Our computational analysis confirmed the dysregulation of key retinoblastoma-associated genes. In comparison to normal retinal tissue, RB1 exhibited a 2.5-fold increase in expression (adjusted p Conclusions: Our analysis reinforces the critical genetic alterations known in retinoblastoma and unveils new avenues for research into the disease’s molecular basis. The discovery of chemoresistance markers and immune-related genes opens potential pathways for personalized treatment strategies. The study’s outcomes emphasize the power of in silico analyses in unraveling complex cancer genomics.

关键词： Retinoblastoma Gene Expression In Silico Study Differentially Expressed Genes Chemoresistance Immune Response Computational Biology

Decoding Retinoblastoma: Differential Gene Expression

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Evaluating Emissions of Nitrous Oxide from Cropland Soils Under Different Rotations in Mato Grosso, Brazil:A Scenario Simulation Study

International Journal of Clinical Medicine（临床医学国际期刊） 2024年第4期 177页

作者： Ahmed Jasim Mahmood Al-Mashhadani franko Shehaj Lianhong Zhou

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Pedosphere 2019年第4期29卷 432-443页

作者： Katharina H. E. MEURER Eric BOENECKE Uwe franko Department of Ecology Swedish University of Agricultural Sciences Uppsala 75007 (Sweden) Department of Soil System Science Helmholtz Center for Environmental Research (UFZ) Halle (Saale) 06120 (Germany)

Expansion of cropland involves immense land use changes, and the resulting intensified management practices have a strong influence on the functioning of the underlying soil. For instance, increased application of nitrogen(N) fertilizer is known to enhance fluxes of nitrous oxide(N2O) from the soil to the atmosphere. The emission factor(EF) proposed by the Intergovernmental Panel on Climate Change(IPCC) assumes a linear relationship between added N and N2O-N fluxes, but it does not account for environmental factors, such as soil properties or climate. Due to the high spatial and temporal variability of N2O-N fluxes, mechanistic models are preferable in terms of extrapolation to larger scales. In this study, we evaluated simulated N2O-N fluxes from soils under agricultural use in the Brazilian state, Mato Grosso, using the CANDY(Carbon and Nitrogen Dynamics) model. A control tool was developed in order to enable the simulation of 1 650 scenarios covering different sites(soil + climate) and management regimes(crop rotation +amount of applied fertilizer + sowing and harvesting dates). Results suggested that the sites had a very strong influence on calculated emissions, which is not accounted for by static EF. Furthermore, most fertilizer-induced N2O-N fluxes derived from the scenario simulations were best described by a non-linear function. For sounder budgeting on the federal and national scale, there is still a strong need for long-term observations of continuous crop rotations and spatial distribution of soil types and their specific characteristics. The presented results provide a valuable starting point for developing further scenario simulations and adapting experimental campaigns for N2O emission study.

关键词： agricultural systems Carbon and Nitrogen Dynamics CANDY model climate emission factor management soil property soil types

Emergency Management in Critical Infrastructures: A Complex-Event-Processing Paradigm

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Journal of Systems Science and Systems Engineering 2019年第1期28卷 37-62页

作者： Vuk Mijovic Nikola Tomasevic Valentina Janev mladen Stanojevic Sanja Vranes The Mihajlo Pupin Institute University of Belgrade Volgina 1511060 Belgrade Serbia

Critical infrastructures (CI) are difficult to handle due to their complexity, size and the number of stakeholders involved. During emergency situations (e.g. fire or terrorist attack), a CI operator in the control room is faced with a flood of information coming from different sensors and legacy monitoring systems. Since in these situations, time is critical and the operators are under a great deal of pressure, a holistic management of all the technical systems and actors involved is needed, reinforced by the Recommendation and Decision Support System (RDSS) that helps emergency managers to take correct and timely decisions. One way to provide an adequate RDSS support to the operator is proposed in this paper which is based on an intelligent, event driven layer that sits on top of the legacy CI monitoring system. Powered by the complex event processing capabilities and facility data model implemented in the form of CI ontology, this layer processes events originating from different sources, conducts the situation and risk assessment, and reacts accordingly, either automatically or via recommendations proposed to emergency personnel. To validate the proposed approach, an event-driven RDSS was deployed on an airport use case (Nikola Tesla airport in Belgrade), as one of the most complex CIs.

关键词： Emergency management event-driven decision support complex event processing emergency personnel training

项目管理的未来趋势:数字化、道德监管与人类决策的适应性

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项目管理技术 2024年第7期22卷 2-4页

作者：马登·武科曼诺维奇周密刘琰 IPMA 克罗地亚萨格勒布大学国际项目管理协会南京大学

在当今快速发展的商业环境中,数字化技术成为推动各产业、各领域变革和创新的重要力量。在项目管理领域,数字化彻底改变了项目管理的方式,使智能、高效地管理项目成为可能。本期前沿论坛,我们与国际项目管理协会(International Project ... 详细信息

在当今快速发展的商业环境中,数字化技术成为推动各产业、各领域变革和创新的重要力量。在项目管理领域,数字化彻底改变了项目管理的方式,使智能、高效地管理项目成为可能。本期前沿论坛,我们与国际项目管理协会(International Project Management Association,IPMA)主席马登·武科曼诺维奇(mladen Vukomanovic)教授进行一场关于项目管理未来发展趋势、项目管理中国贡献及青年项目管理领袖培养的探讨。

关键词：项目管理前沿论坛数字化技术诺维奇未来趋势变革和创新

Modelling Dry Port Systems in the Framework of Inland Waterway Container Terminals

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Computer Modeling in Engineering & Sciences 2023年第10期137卷 1019-1046页

作者： Milovan Kovac Snezana Tadic mladen Krstic Violeta Roso Faculty of Transport and Traffic Engineering University of BelgradeBelgrade11000Serbia Department of Technology Management and Economics Chalmers University of TechnologyGothenburg41296Sweden

Overcoming the global sustainability challenges of logistics requires applying solutions that minimize the negative effects of logistics *** most efficient way of doing so is through intermodal transportation(IT).Current IT systems rely mostly on road,rail,and sea transport,not inland waterway *** dry port(DP)terminals has been proven as a sustainable means of promoting and utilizing IT in the hinterland of seaport container *** DP systems consolidate container flows from/to seaports and integrate road and rail transportation modes in the hinterland which improves the sustainability of the whole logistics *** this article,to extend literature on the sustainable development of different categories of IT terminals,especially DPs,and their varying roles,we examine the possibility of developing DP terminals within the framework of inland waterway container terminals(IWCTs).Establishing combined road–rail–inland waterway transport for observed container flows is expected to make the IT systems *** such,this article is the first to address the modelling of such DP *** mathematically formulating the problem of modelling DP systems,which entailed determining the number and location of DP terminals for IWCTs,their capacity,and their allocation of container flows,we solved the problem with a hybrid metaheuristic model based on the Bee Colony Optimisation(BCO)algorithmand themeasurement of alternatives and ranking according to compromise solution(i.e.,MARCOS)multi-criteria decision-making *** results from our case study of the Danube region suggest that planning and developingDP terminals in the framework of IWCTs can indeed be sustainable,as well as contribute to the development of logistics networks,the regionalisation of river ports,and the geographic expansion of their ***,the main contributions of this article are in proposing a novel DP concept variant,mathematically formulating

关键词： Dry port intermodal transport terminal sustainability Bee Colony Optimization MARCOS inland waterway transport

How to Evaluate Human Capital？

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Chinese Business Review 2012年第9期11卷 830-838页

作者： franko Milost University of Primorska Koper Slovenia

A company is usually founded by individuals striving to achieve their own or broader goals. Goal achievement related to a company＇s operations is called business or the business process. Human capital （man＇s work, employees） is an important element of the business process, however its value is not disclosed on the assets side of the classical balance sheet. In order to shown assets, human capital has to be evaluated. Evaluation can be made in monetary or non-monetary terms. Non-monetary models for evaluating human capital include organisational and behavioural variables. These variables are not expressed in monetary terms, however, based on changes in their quality, one can assume the increased or decreased value of human capital within the company. The value of non-monetary models should not be underestimated, however monetary models are of greater importance. In this article, the most significant non-monetary and monetary models of human capital evaluation are discussed. Among non-monetary models the Michigan, Flamholz, and Ogan models are discussed. Among monetary models the replacement costs model, the opportunity costs model, the discounted wages and salaries model, and originally created dynamic model are discussed. A descriptive approach is used to identify the basic characteristics of existing models for evaluating human capital. According to these findings a different approach is taken in developing an original model. Dynamic model can efficiently overcome most of the practical problems and can be used as an appropriate estimator of human capital value expressed in monetary terms. The research limitations are that the dynamic model has not been sufficiently verified in practice. The model could prove to be directly applicable in those enterprises that would like to define the value of their human capital.

关键词： human resource accounting human capital financial statements models of human capital evaluation