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中国水产科学 2010年第4期17卷 791-797页

作者：张伟王文娟蔡春芳夏燕美 shild krogdahl 马红叶元土苏州大学基础医学与生物科学学院 Aquaculture Protein Centre

以初始体质量约35g的异育银鲫(Allogynogenetic silver crucian carp)(Carassius auratus gibelio♀×Cyprinus carpio♂)为研究对象,以鱼粉为对照饲料,研究了摄食含30%豆粕饲料8周后的生长性能及实验前(0周)、3周和8周后肠道显微结构... 详细信息

以初始体质量约35g的异育银鲫(Allogynogenetic silver crucian carp)(Carassius auratus gibelio♀×Cyprinus carpio♂)为研究对象,以鱼粉为对照饲料,研究了摄食含30%豆粕饲料8周后的生长性能及实验前(0周)、3周和8周后肠道显微结构的变化。结果表明:1)与对照组相比,30%豆粕显著降低鲫鱼的增重率和特定生长率(P<0.05);2)组织切片显示:摄食30%豆粕3周后鲫鱼前、中、后肠较0周时黏膜皱襞受损,边缘糜烂,出现缺刻,皱襞间质变宽且有大量淋巴细胞浸润,黏膜上皮细胞核排列紊乱,肌层明显变薄;3)扫描电镜显示:较0周时前肠和后肠黏膜皱襞间距明显变宽,黏膜皱襞间可见脱落组织,中肠黏膜皱襞间距未见明显变化,但黏膜上皮结构糜烂。8周后肠道受损症状却有所减轻。上述结果表明,异育银鲫对豆粕中致敏因子比较敏感,但随着作用时间延长可产生一定的适应性。本研究旨在为异育银鲫人工饲料配方的优化提供科学依据。

关键词：豆粕异育银鲫肠道组织切片扫描电镜

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Time response expression profiles of genes,possibly associated with effects of altered intestinal microbiota in Atlantic salmon(Salmo salar L.) fed soybean meal

Time response expression profiles of genes,possibly associat...

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The 14th International Symposium on Fish Nutrition & Feeding

作者： Elin C Valen Inderjit Singh Marjara Trond M Kortner shild krogdahl Anne Marie Bakke Aquaculture Protein Centre(a CoE) Department of Basic Sciences and Aquatic MedicineNorwegian School of Veterinary Science

Soybean is often used as an alternative protein source in fish feed,although it is well established that it induces mucosal disorder and inflammatory responses in the distal *** exact cause of soybean meal(SBM)-induced enteritis is so far not fully ***, SBM contains several potent antinutritional factors that disturb the digestive system and thereby cause both reduced weight gain and reduced feed conversion in Atlantic *** have also shown that inclusion of soybean in the feed altered the composition of the intestinal microbiota, both in number and diversity of *** of oxytetracycline in the SBM diets reduced the number of bacteria,but did not affect the severity of the *** a role of intestinal bacteria in the development of the inflammation cannot be excluded and further investigations are *** bowel disease(IBD) is a chronic relapsing inflammatory disorder in the gastrointestinal tract of humans,caused in part by an unregulated immune response to intestinal *** conserved molecules derived from microbes can activate Toll-like receptors(TLRs),and in this way disrupt the intestinal *** via the TLRs leads to the production of a variety of inflammatory cytokines including interferons and interleukins,which can stimulate an increase in T cell proliferation and *** from previous experiments using immunohistochemical and molecular methods indicate an increased presence of T cells during the development of SBM-induced enteritis,possibly linking the TLRs and the altered intestinal microbiota to the inflammatory responses observed after exposure to SBM.

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Intestinal epithelial cell turnover is an adaptive response to diet-induced enteritis in Atlantic salmon(Salmo salar L)

Intestinal epithelial cell turnover is an adaptive response ...

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The 14th International Symposium on Fish Nutrition & Feeding

作者： Elvis M.Chikwati Inger Rudshaug Gunn C.Φstby Fredrik F.Venold Thor Landsverk Stle Refstie Anne Marie Bakke Michael H.Penn shild krogdahl Aquaculture Protein Centre(a CoE) Department of Basic Sciences and Aquatic MedicineNorwegian School of Veterinary Science Section of Anatomy and Pathology Department of Basic Sciences and Aquatic MedicineNorwegian School of Veterinary Science

Inclusion of plant ingredients in fish feeds is often associated with manifestations of wide ranging gastrointestinal(GIT) *** causes remain *** regarding intestinal response mechanisms may contribute to the better understanding of the causes and mechanisms behind these *** epithelial cell(IEC) turnover(proliferation, differentiation,migration and loss) is an important adaptive response of the fish intestine to diverse stimuli such as inflammation,parasitism,chemo- and radio- toxicity.

关键词： Intestinal epithelium Cell proliferation BrdU Soybean meal Atlantic salmon

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