检索结果-南通市图书馆

Journal of zhejiang university-{2. B(Biomedicine & Biotechnology) 2012年第7期13卷 545-554页

作者： Guo-ying FAN ruo-song YAng Jin-qing JIAng Xin-yao CHAng Jun-jie chen Yong-hua QI Shi-xiu WU Xue-feng YAng College of Veterinary Medicine Northwest A&F UniversityYangling 712100China College of Animal science Henan Institute of Science and TechnologyXinxiang 453003China

Modified 1-ethyl-3.(3.dimethylaminopropyl)carbodiimide(EDC)method was employed to synthesize the artificial antigen of norfloxacin(NOr),and New Zealand rabbits were used to produce anti-NOr polyclonal antibody(pAb).Based on the checkerboard titration,an indirect competitive enzyme-linked immunosorbent assay(icELISA) standard curve was *** assay was sensitive and had a working range from 0.12.to 68.40 ng/ml,with the half maximal inhibitory concentration(IC50)and limit of detection(LOD)values of 2.7 ng/ml and 0.06 ng/ml,*** produced pAb exhibited high cross-reactivity to fluoroquinolones(FQs)tested,and the IC50 values to enoxacin,ciprofloxacin,and pefloxacin were 3.1,3.4,and 4.1 ng/ml,*** also indicated that the concentrations of NaOH and methanol in assay buffer should not be higher than 10%and 3.%.When spiked in milk at 5,2.,and 50 ng/ml,the recoveries for NOr,enoxacin,ciprofloxacin,and pefloxacin ranged 90.5%-98.0%,84.0%-95.2.,94.0%-106.0%,and 89.5%-100.0%,*** results suggest that this class-specific pAb-based icELISA could be utilized for the primary screening of FQ residues in animal-original products.

关键词： Norfloxacin Fluoroquinolones Indirect competitive ELISA Class-specificity Milk

在线全文

学校读者我要写书评

暂无评论

大黄素对急性肾损伤大鼠肠道菌群的调节作用

中国中药杂志 2019年第4期44卷 758-764页

作者：孙蒋罗静雯姚文杰骆晓婷苏辰霖魏颖慧浙江中医药大学药学院浙江杭州311402

该文研究大黄素对急性肾损伤(AKI)大鼠肠道菌群的调节作用。3.只健康雄性大鼠随机分为正常组、模型组、阳性对照组(盐酸贝那普利5 mg·kg^(-1))和大黄素低(10 mg·kg^(-1))、中(2. mg·kg^(-1))、高(50 mg·kg^(-1))剂量组。除正常组外... 详细信息

该文研究大黄素对急性肾损伤(AKI)大鼠肠道菌群的调节作用。3.只健康雄性大鼠随机分为正常组、模型组、阳性对照组(盐酸贝那普利5 mg·kg^(-1))和大黄素低(10 mg·kg^(-1))、中(2. mg·kg^(-1))、高(50 mg·kg^(-1))剂量组。除正常组外,其余各组采用腹腔注射硫酸庆大霉素损伤肾组织构建急性肾损伤模型,连续注射7 d,1次/d,腹腔注射2.h后,除模型组和正常组用生理盐水灌胃,其余各组分别用相应剂量药物灌胃给药,持续给药15 d。收集各组大鼠尿液和粪便分别测定尿蛋白总量和4种菌属含量;采集各组大鼠血液进行血液生化指标测定; HE染色观察各组大鼠肾脏组织形态学改变。结果显示,与正常组相比,模型组体质量、尿蛋白总量(UTP)、血浆细菌内毒素、尿素氮(BUN)、肌酐(SCr)、血清D-乳酸水平以及4种细菌含量均有显著变化(P<0. 05);与模型组相比,阳性对照组和大黄素各剂量组尿蛋白总量、尿素氮、血清D-乳酸水平、肌酐和血浆细菌内毒素均有所下降,以大黄素高剂量组下降最为显著(P<0. 01),肾脏病理修复情况最好;除大黄素低剂量组外,各给药组大肠杆菌及肠球菌数量显著下降(P<0. 05),乳酸菌及双歧杆菌明显升高(P<0. 05),以大黄素高剂量组变化最为明显(P<0. 01)。研究发现急性肾损伤大鼠(AKI)存在较明显的肠道菌群失调,大黄素对AKI大鼠肠道菌群失衡有一定的调节作用,这可能是其治疗急性肾损伤的作用机制之一。

关键词：大黄素急性肾损伤肠道菌群荧光定量PCr

Cholangiocarcinoma-derived exosomes inhibit the antitumor activity of cytokine-induced killer cells by down-regulating the secretion of tumor necrosis factor-α and perforin

在线全文

学校读者我要写书评

暂无评论

Journal of zhejiang university-{2. B(Biomedicine & Biotechnology) 2016年第7期17卷 537-544页

作者： Jiong-huang {2. Jian-yang XIAng Guo-ping DIng Li-ping CAO dep.r.ment of General Surgery Sir Run Run Shaw Hospital School of Medicine Zhejiang University

Objective： The aim of our study is to observe the impact of cholangiocarcinoma-derived exosomes on the antitumor activities of cytokine-induced killer （CIK） cells and then demonstrate the appropriate mechanism. Methods： Tumor-derived exosomes （TEXs）, which are derived from rBE cells （human cholangiocarcinoma line）, were collected by ultracentrifugation. CIK cells induced from peripheral blood were stimulated by TEXs. Fluorescence-activated cell sorting （FACS） was performed to determine the phenotypes of TEX-CIK and N-CIK （normal CIK） cells. The concen- trations of tumor necrosis factor-a （TNF-a） and perforin in the culture medium supematant were examined by using an enzyme-linked immunosorbent assay （ELISA） kit. A CCK-8 kit was used to evaluate the cytotoxic activity of the CIK cells to the rBE cell line. results： The concentrations of TNF-a and perforin of the group TEX-CIK were 13..61 pg/ml and 2.41 ng/ml, respectively, lower than those of the group N-CIK 194.08 pg/ml （P〈0.01） and 3.3. ng/ml （P〈0.05）. The killing rate of the group TEX-CIK was 3..3.%, lower than that of the group N-CIK （47,3.% （P〈0.01））. The population of CD3., CD8＋, NK （CD56＋）, and CD3.CD56＋ cells decreased in the TEX-CIK group （63.2.6.8）%, （2.5±1.0）%, （0.53.0.49）%, （0.45±0.42.%） compared with the N-CIK group （（90.3.7.3.%, （65.7±3.3.%, （4.2.1.2.%, （15.2.2.7）%）, P〈0.01. Conclusions： Our results suggest that rBE cells-derived exosomes inhibit the antitumor activity of CIK cells by down-regulating the population of CD3., CD8＋, NK （CD56＋）, and CD3.CD56＋ cells and the secretion of TNF-a and perforin. TEX may play an important role in cholangiocarcinoma immune escape.

关键词： Cholangiocarcinoma Tumor-derived exosomes Cytokine-induced killer cells Immune escape

Simultaneous determination by UPLC-MS/MS of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets: application to a pharmacokinetic study

在线全文

学校读者我要写书评

暂无评论

Journal of zhejiang university-{2. B(Biomedicine & Biotechnology) 2014年第11期15卷 929-939页

作者： Wen-ping WAng Na LIU Qian KAng Pei-pei DU Yi LAN Bo-{3. ZHAO Yan-yan {3. Qing ZHAng Hui LI Ye-wen ZHAng Qing WU School of Chinese material Medica Beijing University of Chinese Medicine Beijing Handian Pharmaceutical Co. Ltd.

A rapid, reliable, and sensitive method was developed using ultra-performance liquid chromatography- tandem mass spectrometry （UPLC-MS/MS） with an electrospray ionization （ESI） source for determination of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets （GBTs）. The method simultane- ously detects bilobalide （BB）, ginkgolide A （GA）, ginkgolide B （GB）, ginkgolide C （GC）, quercetin （QCT）, kaempferol （KMF）, and isorhamnetin （ISr） for pharmacokinetic study. The analytes and internal standard （IS） were extracted from rat plasma by acetidin. An MS/MS detection was conducted using multiple reaction monitoring （MrM） and operating in the negative ionization mode. The calibration curve ranges were 5-500, 5-500, 2.5-2.0, 1-100, 1-100, 1-100, and 1-100 ng/ml for BB, GA, GB, GC, QCT, KMF, and ISr, respectively. The mean recovery of the analytes ranged from 68.11% to 84.42.. The intra- and inter-day precisions were in the range of 2.3.%-9.86% and the accuracies were between 87.67% and 108.3.%. The method was used successfully in a pharmacokinetic study of GBTs. The phar- macokinetic parameters of seven compounds were analyzed using a non-compart{13. model. Plasma concentrations of the seven compounds were determined up to 48 h after administration, and their pharmacokinetic parameters were in agree{13. with previous studies.

关键词： Ginkgo biloba tablet Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) Pharmacokinetics

混合工质天然气液化流程的特色结构与性能综述（英文）

在线全文

学校读者我要写书评

暂无评论

Journal of zhejiang university-{2. A(Applied Physics & {3.) 2019年第10期20卷 727-780页

作者： Qi SOng Jing-peng ZHAng Zhen ZHAO Jie-lin LUO Qin WAng Guang-ming chen Key Laboratory of refrigeration and Cryogenic Technology of zhejiang Province Institute of Refrigeration and Cryogenics Zhejiang University

天然气的运输与储存均以液态(Lng)为宜。近几十年来,随着混合制冷剂天然气液化流程的飞速发展,出现了种类繁多的结构形式,为业界提供了丰富的选择。针对种类繁多的混合制冷剂天然气液化流程,本文提出了一个新的分类框架,并在此基础上介... 详细信息

天然气的运输与储存均以液态(Lng)为宜。近几十年来,随着混合制冷剂天然气液化流程的飞速发展,出现了种类繁多的结构形式,为业界提供了丰富的选择。针对种类繁多的混合制冷剂天然气液化流程,本文提出了一个新的分类框架,并在此基础上介绍了每一类中具有特色的商业化流程或专利设计中的结构特点;同时,整理和讨论了这些流程所采用的制冷剂组合和运行参数(包括运行压力、每一级的制冷温度和蒸发压力位的数量等),并将单位液化功作为最重要的性能指标。本综述旨在从流程结构的角度厘清混合制冷剂天然气液化流程的发展历程,以及展示流程之间结构性的区别,为从业人员选择合适流程提供参考,同时也为已有流程性能的优化以及新流程的构建提供依据与建议。

关键词：天然气液化混合制冷剂结构性能

Long-Term Maintenance of Luteinizing Hormone –responsive Testosterone Formation by Primary Leydig Cells in Vitro

在线全文

学校读者我要写书评

暂无评论

Long-Term Maintenance of Luteinizing Hormone –Responsive Te...

The SUPEr reporting guideline suggested for reporting of surgical technique

中国药理学会第十届全国基础与临床生殖药理学术研讨会暨2.17育英生殖药理论坛

作者： Yiyan Wang Fenfen chen June Liu Janet Folmer Barry Zirkin Haolin chen renshan Ge The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical university dep.r.ment of Biochemistry and Molecular Biology Johns Hopkins Bloomberg School of Public Health

The inability of cultured primary Leydig cells to maintain luteinizing hormone(LH)-responsive testosterone formation in vitro for more than 3.5 days has presented a major challenge in testing the effects of trophic regulatory factors or chronic exposure to environ{3.al toxicants on these critically important *** the present study, our major objective was to establish culture conditions sufficient to maintain LH-responsive testosterone formation for up to a month. The formula contains a mixture of DMEM/F12.and M199 culture medium, containing insulin(10 ug/ml), PDGFAA(10 ng/ml), lipoprotein(0.1 mg/ml), horse serum(1%) and a submaximal amount of LH(0.2.ng/ml). Under this culture condition, adult rat Leydig cells can be maintained in vitro for up to 4 weeks, with littledivision, but retained the ability to respond to LH stimulation and to produce testosterone. As a prove-principle purpose, we have used the culture system tested the chronic effect of a common environ{3.al-relavent compound, MEHP, on Leydig cell testosterone production. The results indicated that while MEHP, at the concentration of 1 uM, did not affect Leydig cell steroidogenesis acutely when incubated with the cells for 2. hours, it indeed significantly inhibited T production when the cells were exposed to the compound for two weeks. The results suggest that the new culture condition we have established is useful to screen and test the potential chronic effects of environ{3.al toxins on Leydig cells.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Hepatobiliary Surgery and Nutrition 2023年第4期12卷 534-544,I0024-I0026页

作者： Kaiping Zhang Yanfang Ma Jinlin Wu Qianling Shi Leandro Cardoso Barchi Marco Scarci rene Horsleben Petersen Calvin S.H.ng Steven Hochwald ryuichi Waseda Fabio Davoli robert Fruscio Giovanni Battista Levi Sandri Michel Gonzalez Benjamin Wei Guillaume Piessen Jianfei Shen Xianzhuo Zhang Panpan Jiao Yulong He Nuria M.Novoa Benedetta Bedetti Sebastien Gilbert Alan D.L.Sihoe Alper Toker Alfonso Fiorelli Marcelo F.Jimenez Toni Lerut Aung Y.Oo Grace S.Li Xueqin Tang Yawen Lu Hussein Elkhayat Tomaz Stupnik Tanel Laisaar Firas Abu Akar Diego Gonzalez-rivas Zhanhao Su Bin Qiu Stephen D.Wang Yaolong chen Shugeng Gao Editorial Office AME Publishing CompanyHong KongChina School of Chinese Medicine Hong Kong Baptist UniversityHong KongChina dep.r.ment of Cardiovascular Surgery Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhouChina Evidence-based Medicine Center School of Basic Medical SciencesLanzhou UniversityLanzhouChina Lanzhou university Institute of Health Data science LanzhouChina Digestive Surgery Division Department of GastroenterologyUniversity of Sao Paulo School of MedicineSão PauloBrazil dep.r.ment of Cardiothoracic Surgery Imperial College Healthcare NHS TrustLondonUK dep.r.ment of Cardiothoracic Surgery University Hospital of CopenhagenRigshospitaletCopenhagenDenmark The Chinese university of Hong Kong Prince of Wales HospitalShatinNew TerritoriesHong KongChina dep.r.ment of Surgical Oncology Roswell Park Cancer InstituteBuffaloNYUSA dep.r.ment of General Thoracic Breast and Pediatric SurgeryFukuoka UniversityFukuokaJapan dep.r.ment of Thoracic Surgery AUSL RomagnaS.Maria delle Croci Teaching HospitalRavennaItaly Clinic of Obstetrics and Gynecology University of Milan-BicoccaSan Gerardo HospitalMonzaItaly Division of General Surgery and Liver Transplantation S.Camillo HospitalRomeItaly Service of Thoracic Surgery Lausanne University Hospital(CHUV)LausanneSwitzerland Division of Cardiothoracic Surgery Department of SurgeryUniversity of Alabama BirminghamBirminghamALUSA university of Lille CNRSInsermCHU LilleUMR9020-U1277-CANTHER-Cancer Heterogeneity Plasticity and Resistance to TherapiesLilleFrance dep.r.ment of Cardiothoracic Surgery Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityLinhaiChina The First School of Clinical Medicine Lanzhou UniversityLanzhouChina Thoracic Surgery Service University Hospital of SalamancaBiomedical Institute of SalamancaSalamancaSpain dep.r.ment of Thoracic Surgery Helios Clinic Bonn/Rhein SiegBonnGermany Division of Thoracic Surgery Department of SurgeryThe Ottawa

Background:Existing reporting guidelines pay insufficient attention to the detail and comprehensiveness reporting of surgical *** Surgical techniqUe rePorting chEcklist and standards(SUPEr)aims to address this gap by defining reporting standards for surgical *** SUPEr guideline intends to apply to articles that encompass surgical technique in any study design,surgical discipline,and stage of surgical ***:Following the EQUATOr(Enhancing the QUAlity and Transparency Of health research)Network approach,16 surgeons,journal editors,and methodologists reviewed existing reporting guidelines relating to surgical technique,reviewed papers from 15 top journals,and brainstormed to draft initial items for the *** initial items were revised through a three-round Delphi survey from 2. multidisciplinary Delphi panel experts from 13.countries and *** final SUPEr items were formed after an online consensus meeting to resolve disagreements and a three-round wording refinement by all 16 SUPEr working group members and five SUPEr ***:The SUPEr reporting guideline includes 2. items that are considered essential for good and informative surgical technique *** items are divided into six sections:background,rationale,and objectives(items 1 to 5);preoperative preparations and requirements(items 6 to 9);surgical technique details(items 10 to 15);postoperative considerations and tasks(items 16 to 19);summary and prospect(items 2. and 2.);and other information(item 2.).Conclusions:The SUPEr reporting guideline has the potential to guide detailed,comprehensive,and transparent surgical technique reporting for *** may also assist journal editors,peer reviewers,systematic reviewers,and guideline developers in the evaluation of surgical technique papers and help practitioners to better understand and reproduce surgical technique.

关键词： Surgical technique surgical innovation reporting guideline reporting checklist Surgical techniqUe rePorting chEcklist and standards(SUPEr)

新碳源制备活性炭和石墨烯及其非等电极电容水系超级电容器

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

物理化学学报 2020年第2期36卷 123-141页

作者：陈尧陈政武汉科技大学材料与冶金学院省部共建耐火材料与冶金国家重点实验室武汉430081 宁波诺丁汉大学科学与工程学院化学与环境工程系浙江宁波315100 诺丁汉大学工程学院化学与环境工程系英国诺丁汉NG27RD

碳材料具有不同的微米和纳米结构以及本体和表面的官能基团,因此成为最普遍采用的超级电容器电极材料。典型的例子是活性炭和石墨烯。最近的研究趋势是通过新方法,以传统和新碳源,例如生物质、聚合物、氧化石墨、碳氢以及二氧化碳气体,... 详细信息

碳材料具有不同的微米和纳米结构以及本体和表面的官能基团,因此成为最普遍采用的超级电容器电极材料。典型的例子是活性炭和石墨烯。最近的研究趋势是通过新方法,以传统和新碳源,例如生物质、聚合物、氧化石墨、碳氢以及二氧化碳气体,来制备成本低、电容性能高的活性炭和石墨烯。特别是,大多数新碳源衍生碳非常适用于水系电解液。电荷存储不仅发生在"碳|电解液"界面上(形成双电层),也依靠本体和表面的官能化带来的氧化还原活性,包括有限离域价电子转移反应。此外,进一步理解电荷存储机制有助于设计出比传统对称电容器具有更高电压和比能量的非等电极电容水系超级电容器。本文综述了新碳源衍生碳材料和器件的最新进展,为超级电容器技术的持续发展助力。

关键词：新碳源活性炭石墨烯水系电容器非等电极电容

在线全文

学校读者我要写书评

暂无评论

杭州市水源中藻类衍生嗅味污染及其释放特征

浙江大学学报（工学版） 2014年第5期48卷 858-864页

作者：马晓雁李军李青松倪永炯陈晨高乃云浙江工业大学建筑工程学院市政工程研究所浙江杭州310014 厦门理工学院水资源环境研究所福建厦门361005 同济大学环境科学与工程学院上海200092

分别采用显微镜鉴别、计数法和固相微萃取-气质联用(SPME-GC/MS)分析方法对杭州市区饮水水源中藻类构成和部分藻类衍生嗅味污染物的分布特征进行调查,并展开主要嗅味污染物的藻源性分析.结果表明,水源水中藻类以硅藻和绿藻为构成主体,... 详细信息

分别采用显微镜鉴别、计数法和固相微萃取-气质联用(SPME-GC/MS)分析方法对杭州市区饮水水源中藻类构成和部分藻类衍生嗅味污染物的分布特征进行调查,并展开主要嗅味污染物的藻源性分析.结果表明,水源水中藻类以硅藻和绿藻为构成主体,伴有季节性变化特征.β-环柠檬醛(β-cyclocitral)是最易检出嗅味污染物,最高检出质量浓度51.4ng/L,其他嗅味污染物2.甲基异茨醇(2.MIB),二甲基三硫醚(DMTS)和土臭素(GSM)等检出质量浓度较低,但峰值浓度已超出嗅阈值.β-cyclocitral的藻源性研究结果表明,铜绿微囊藻释放的嗅味污染物以β-cyclocitral为主,另含有少量的DMTS和β-紫罗兰酮(β-ionone).藻细胞质量浓度为106 L-1时,水中β-cyclocitral质量浓度最高可达44.0ng/L,通过细胞破碎测定胞内质量浓度表征释放潜能,最高可达174.8ng/L.

关键词：水源水藻类衍生污染嗅味污染物 β-环柠檬醛铜绿微囊藻

一类Duffing型系统的不动点混沌和1Fold/Fold簇发现象及机理分析

在线全文

学校读者我要写书评

暂无评论

力学学报 2020年第5期52卷 1475-1484,I0006页

作者：陈娅昵孟文静钱有华浙江师范大学数学与计算机科学学院浙江金华321004

本文主要探究了一类含有两个慢变量的双稳态Duffing型系统,通过时间历程图、相图、分岔图等对系统进行数值模拟,然后从理论上分析不同参数下系统的动力学机理.首先,研究发现当振幅参数取值大于1时,系统会表现出不动点混沌现象,并进一步... 详细信息

本文主要探究了一类含有两个慢变量的双稳态Duffing型系统,通过时间历程图、相图、分岔图等对系统进行数值模拟,然后从理论上分析不同参数下系统的动力学机理.首先,研究发现当振幅参数取值大于1时,系统会表现出不动点混沌现象,并进一步解释了产生不动点混沌的机理.其次,介绍了参数空间中的簇发振荡现象,即系统穿过鞍结曲面的一侧到达另一侧所发生的行为,这里也称为鞍结簇发振荡.事实上,当系统穿过鞍结曲面的时候,它的平衡点个数发生了变化.然后,使用纵向抛物线路径说明了Fold/Fold簇发振荡产生的机理,发现无论常系数项和振幅的取值为多少,只要满足一定的关系,总会产生Fold/Fold簇发振荡,之后使用线性路径阐明了新增常系数项会使得系统发生簇发振荡的原因.并且发现路径与鞍结曲面交点的位置会影响簇发振荡的对称性;路径的跨度会影响簇发振荡的大小.最后,使用多拐折曲线路径讨论当两个激励项存在n倍关系时系统产生的现象.结果表明当n=3.,常系数项的变化会使得系统表现出不同重数的Fold/Fold簇发振荡,最高可达到三重簇发振荡.并且发现在理想状况下如果可以找到一条路径可以分割为n段,并且每一段都会与鞍结曲面有交点,那么会产生n重Fold/Fold簇发振荡.

关键词： Fold/Fold簇发振荡 Duffing系统参数空间不动点混沌