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Determination of Steroid Hormone residues in Fish Tissues Using Gel Permeation Chromatography and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

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Journal of Ocean university of China 2018年第5期17卷 1171-1177页

作者： LI Peipei YAN Zhongyong SUN Xiumei {2. Si {2. Yin ZHAng Xiaojun Marine Fishery Institute of zhejiang Province Zhoushan 316021China Key Laboratory of Sustainable Utilization of Technology research for Fishery resource of zhejiang Province Zhoushan 316021China zhejiang Ocean university Zhoushan 316022China

A highly sensitive method was developed for the simultaneous determination of 8 steroid hormones in high-fat fish tissues using ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The 8 steroid hormones were extracted from the tissues with diethyl *** from other common purification methods,the extract solutions were cleaned by gel permeation chromatography(GPC) using ethyl acetate-cyclohexane solution(1:1,v/v) as the mobile *** separation of target compounds was carried out by a BEH C18 column and a gradient elution consisting of acetonitrile and 0.{3. aqueous formic acid(v/v).The compounds were detected under the multiple reaction monitoring(MrM) mode and quantified with external standard *** method was validated with respect to linearity,specificity,accuracy and precision.A linearity with correlation coefficient larger than 0.995 was achieved in the range of 0.5 to 50 ng m L^(-1).The average recoveries at the spiked levels of 1.0,5.0,and 10.0 μg kg^(–1) varied between 81.7% and 90.8%,with the relative standard deviations(n=5) ranged from 3.50% to 10.0%.The limit of quantification(LOQ) for 8 steroid hormones ranged from 0.{3.to 1.5 μg kg^(-1).It was concluded that this method can be successfully applied for the determination of 8 steroid hormones in complicated matrices including high-fat fish tissues.

关键词： ultra performance liquid chromatography-tandem mass spectrometry anabolic hormone gel permeation chromatography fish tissue

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Effects of epidermal growth factor on transforming growth factor-beta1-induced epithelial-mesenchymal transition and potential mechanism in human corneal epithelial cells

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International Journal of Ophthalmology(English edition) 2020年第1期13卷 11-20页

作者： Shu-Yang chen chen Xie Hong Zhu Ye Shen dep.r.ment of Ophthalmology First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310003Zhejiang ProvinceChina Tongde Hospital of zhejiang Province Hangzhou 310012Zhejiang ProvinceChina Clinical research Center the First Affiliate HospitalSchool of MedicineZhejiang UniversityHangzhou 310003Zhejiang ProvinceChina

AIM: To evaluate the effects of epidermal growth factor(EGF) on transforming growth factor-beta1(TGF-β1)-induced epithelial-mesenchymal transition(EMT) in human corneal epithelial cells(HCECs). METHODS: HCECs were cultured and treated with TGF-β1 for establishing the model of EMT in vitro. Biological effect of EGF on TGF-β1-induced EMT was evaluated. Proteins and m rNAs expression changes of E-cadherin, N-cadherin and Fibronectin(EMT-relative markers) after TGF-β1 or TGF-β1 combined EGF treat{3. were detected by Western blot and rT-PCr, respectively. Viability and migration of HCECs were measured by CCK-8, transwell cell migration assay and cell scratch wound healing assay. Activation of Smad2. ErK, p3., JNK and Akt signaling pathways were evaluated by Western blot. Inhibitors of relevant signaling pathways were added to the HCECs to explore the key signal ***: With treat{3. of TGF-β1 only, three EMTrelative proteins and m rNA expression showed that EMT up-regulated in a concentration-dependent and time-dependent manner, with significantly decreasing cell viability(TGF-β1≥5 ng/m L, Preasing cell migration(TGF-β1≥5 ng/m L, Prylation of Smad2.and p3. was a key process of TGF-β1-induced EMT. Meanwhile, EMT-relative proteins and m rNA expression showed that EGF inhibited TGF-β1-indued EMT, with significantly increasing cell viability(EGF≥10 ng/m L, Prthy that EGF significantly enhanced cell migration although EMT was inhibited(EGF≥10 ng/m L, P3.(by SB2.2.90, a p3. inhibitor) was a potential mechanism of this phenomenon. CONCLUSION: EGF inhibits TGF-β1-induced EMT via suppressive p3., and promotes cells proliferation and migration in a non-EMT process by inhibiting p3. pathway.

关键词： epidermal growth factor p3. epithelialmesenchymal transition corneal epithelial cell

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Prediction of Thermal Conductivity of Various Nanofluids with Ethylene Glycol using Artificial Neural Network

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Journal of Thermal science 2020年第6期29卷 1504-1512页

作者： WAng Xuehui YAN Xiaona GAO Neng {2. Guangming Fluids and Thermal engineer.ng research Group Faculty of EngineeringUniversity of NottinghamNG72RDUK School of Landscape Architecture Zhejiang A&F UniversityHangzhou 311300China Ningbo Institute of Technology Zhejiang UniversityNingbo 315100China

The nanofluid has been widely used in many heat transfer areas due to its significant enhancement effect on the thermal ***,the methods that can accurately predict their thermal conductivities are very important to evaluate and analyze the heat transfer *** this paper,a novel artificial neural network(ANN)model was proposed to predict the thermal conductivity of nanofluids with ethylene glycol and could be used in a wide range with excellent accuracy.A total of 3.1 experimental data with a wide range of temperatures(4℃ to 90℃),nanoparticles(metal,metal oxide,etc.),volume concentrations(0.05%to 10%),and particle sizes(2.nm to 2.2.nm)were *** build the ANN model,the temperature,thermal conductivities of the base fluid and nanoparticles,the size and volume concentration of the nanoparticles were selected and used as the input *** were 5 nodes,10 nodes and 1 node in input layer,hidden layer and output layer,*** predicted results of the ANN model coincided with the experimental data very well with the correlation coefficient and mean square error(MSE)were 0.9863.and 3.01×10–5,*** relative deviations of 99.74%data were within±5%.The model was expected to be a good practical method to predict the thermal conductivity of nanofluids with ethylene glycol.

关键词： thermal conductivity nanofluids ANN model heat transfer

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Silicon prepared by electro-reduction in molten salts as new energy mater.als

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Journal of Energy Chemistry 2020年第8期29卷 46-61,I0002页

作者： Tingting Jiang Xinyi Xu George Z.{3. The State Key Laboratory of refractories and Metallurgy College of Materials and MetallurgyWuhan University of Science and TechnologyWuhan 430081HubeiChina Electrochemical Technologies Group Faculty of EngineeringUniversity of NottinghamNottingham NG27RDUK Electrochemical Technologies Group Faculty of Science and EngineeringUniversity of Nottingham Ningbo ChinaNingbo 315100ZhejiangChina

Silicon has a large impact on the energy supply and economy in the modern world. In industry, high purity silicon is firstly prepared by carbothermic reduction of silica with the produced raw silicon being further refined by a modified Siemens method. This process suffers from the disadvantages of high cost and contaminant release and emission. As an alternative, the molten salt electrolysis approach, particularly the FFC Cambridge Process(FFC: Fray-Farthing-{2.), could realize high purity silicon products with morphology-controllable nanostructures at low or mild temperatures(generally 650–900 ℃). In this article, we review the develop{3., reaction mechanisms, and electrolysis conditions of silicon production by the FFC Cambridge Process. Applications of the silicon products from electrolysis in molten salts are also discussed in terms of energy applications, including using them as the photovoltaic ele{3. in solar cells and as the charge storage phase in the negative electrode(negatrode) of lithium ion batteries.

关键词： Electrolysis Molten salt Silicon Nanomaterial Li ion battery Solar cell

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Asymptotic Optimality of the Nonnegative Garrote Estimator Under Heteroscedastic Errors

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Journal of Systems science & Complexity 2020年第2期33卷 545-562页

作者： chen Xiuping CAI Guanghui GAO Yan ZHAO Shangwei College of Statistics and Mathematics Zhejiang Gongshang UniversityHangzhou 310018China dep.r.ment of Statistics College of ScienceMinzu University of ChinaBeijing 100081China

This paper proposes the Nonnegative Garrote(ng)estimator for linear model with heteroscedastic *** the other hand,under some regularity conditions,the authors show the asymptotic optimality of the ng estimator by referring to the idea of the asymptotic optimality of the model average *** results and a real data analysis are reported for testing the results obtained *** results provide a stronger theoretical basis for the use of ng estimator by strengthening existing findings.

关键词： Asymptotic optimality coefficient shrinkage heteroscedastic errors Nonnegative Garrote

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茜草植物染料染色羊绒纤维的摩擦牢度

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现代纺织技术 2024年第2期32卷 105-111页

作者：俞建伟侯战昌陈超余志成浙江理工大学纺织科学与工程学院杭州310018 浙江理工大学生态染整技术教育部工程研究中心杭州310018 宁波康赛妮新纤维科技有限公司宁波315200

茜草是最常用的红色天然染料,广泛应用于真丝和纤维素纤维染色。但生产中发现明矾作为媒染剂用于茜草植物染料染色羊绒纤维的摩擦牢度极差。通过分析媒染、染色前后羊绒表面沉积物及其与摩擦牢度的相互关系,探讨了导致摩擦牢度低下的原... 详细信息

茜草是最常用的红色天然染料,广泛应用于真丝和纤维素纤维染色。但生产中发现明矾作为媒染剂用于茜草植物染料染色羊绒纤维的摩擦牢度极差。通过分析媒染、染色前后羊绒表面沉积物及其与摩擦牢度的相互关系,探讨了导致摩擦牢度低下的原因并提出改进方法。结果表明:适度去鳞片处理可有效减少明矾媒染时氢氧化铝在羊绒表面沉积,使茜草植物染料染色羊绒的干、湿摩擦牢度分别提高到2.和3.;酒石酸-明矾媒染羊绒表面无明显沉积,茜草植物染料染色后羊绒纤维的干、湿摩擦牢度分别提高到4级和5级,同时日晒、皂洗牢度也均在4级以上。

关键词：茜草植物染料羊绒摩擦牢度酒石酸-明矾

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一种基于聚集诱导发光机制的新型氨肽酶N荧光探针

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天津大学学报（自然科学与工程技术版） 2024年第7期57卷 704-710页

作者：闫喜龙张子卓李明瑞沈华伟陈立功王博威天津大学化工学院天津300350 天津大学浙江绍兴研究院绍兴312300 东莞理工学院资产与实验室管理处东莞523808 绍兴兴欣新材料股份有限公司绍兴312369

氨肽酶N(APN)在人体中广泛存在,在肿瘤细胞表面大量表达,并在癌症侵袭、转移和血管生产过程中具有重要作用;体液中的APN可作为炎症、癌症的早期生物标志物.鉴于此,发展高灵敏度、高选择性的检测手段对APN进行实时检测及可视化成像将为AP... 详细信息

氨肽酶N(APN)在人体中广泛存在,在肿瘤细胞表面大量表达,并在癌症侵袭、转移和血管生产过程中具有重要作用;体液中的APN可作为炎症、癌症的早期生物标志物.鉴于此,发展高灵敏度、高选择性的检测手段对APN进行实时检测及可视化成像将为APN相关疾病的诊断及病理生理学说明提供可能性.荧光探针因其选择性好、灵敏度高的优势,近年来已被广泛应用于生物酶检测.然而已报道用于检测APN的荧光探针多基于平面分子,易受聚集荧光猝灭(ACQ)限制.与基于传统平面分子的ACQ荧光染料相比,具有聚集诱导发光(AIE)效应的荧光分子能更好地应用于高浓度与高含水量的检测环境,为荧光探针技术的发展开辟了新的路径,而基于AIE机制的APN荧光探针仍鲜有报道.喹啉-丙二腈(QM)类化合物具有长发射波长,且生物相容性好、光稳定性优异,目前已有多种基于QM母体的AIE探针应用于细胞成像与各种活性物质检测.基于此,设计合成了以QM为母体的AIE荧光探针QM-Ala,探针具有99 nm的大斯托克斯位移、良好的稳定性、抗干扰能力和较快的响应速度,检测限(LOD)低至1.2. ng/***-Ala在3.7区间对pH有即时响应,并在体液pH条件下对APN质量浓度有良好的线性响应,因此有望应用于酶抑制剂筛选以及体液中的APN质量浓度检测.

关键词：荧光探针氨肽酶N 聚集诱导发光喹啉-丙二腈

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rational groundwater table indicated by the ecophysiological parameters of the vegetation:A case study of ecological restoration in the lower reaches of the Tarim river

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Chinese science Bulletin 2006年第A01期51卷 8-15页

作者： chen Yaning WAng Qiang LI Weihong rUAN Xiao chen Yapengr ZHAng Lihua Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences Urumqi 830011 China Ningbo Institute of Technology Zhejiang University Ningbo 315100 China

Populus euphratica Oliv 和 Tamarix ramosissima Ldb 的 eco 生理的反应和改编在水版本时期期间被调查。九个典型区域和 45 横断沿着 Tarim 河的更低的活动范围被选择。地下水桌子以及植物表演和脯氨酸，可溶的糖，和植物的内容内长... 详细信息

Populus euphratica Oliv 和 Tamarix ramosissima Ldb 的 eco 生理的反应和改编在水版本时期期间被调查。九个典型区域和 45 横断沿着 Tarim 河的更低的活动范围被选择。地下水桌子以及植物表演和脯氨酸，可溶的糖，和植物的内容内长的荷尔蒙(骆驼毛的织物， CTK ) 在叶子被监视并且分析。地下水桌子在不同区域被提起，横断走水路推出程序。生理的压力到 P。euphratica 和 T。ramosissima 在水版本以后被减少了。我们的结果建议在学习区域的地下水桌子在 3.15～4.12. m 留下了，从 9.2.～11.06 的脯氨酸内容( mmol/L )，从 2.4.71～2.2.16 的可溶的糖内容( mmol/L )，从 3.59～5.01 的 ABA 内容( ng/g FW )，并且从 4.01～4.56 的 CK 内容( ng/g FW )，为最佳生长和 P 的恢复。植物性能参数和水申请的效率显示的 euphratica 最高。在学习区域的地下水桌子在 2.16～3.3. m 留下了，从 12.15～14.17 的脯氨酸内容( mmol/L )，从 154.71～183.16 的可溶的糖内容( mmol/L )，从 2.78～4.86 的 ABA 内容( ng/g FW )，并且从 3.78～4.2. 的 CK 内容( ng/g FW )，为最佳生长和 T 的恢复。植物表演参数和水申请的效率显示的 ramosissima 最高。为在学习区域的植被的恢复的合理地下水桌子在 3.15 ～ 3.3. m。

关键词：内陆河地下水位植物生理响应塔里木河

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超高效液相色谱质谱法同时测定苯磺酸氨氯地平片中的苯磺酸甲酯和苯磺酸乙酯

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温州大学学报（自然科学版） 2024年第1期45卷 33-41页

作者：蔡志威梁键谋陈超陈悦鲍立曾劳伦·鲁尔曼浙江省食品药品检验研究院浙江省药品接触材料质量控制研究重点实验室浙江杭州310052 浙江得恩德制药股份有限公司浙江新昌312560 斯特拉斯堡大学化学研究所法国斯特拉斯堡67081

建立苯磺酸氨氯地平片中苯磺酸甲酯和苯磺酸乙酯超高效液相色谱质谱的检测方法.采用梯度洗脱法同时对苯磺酸甲酯和苯磺酸乙酯进行检测,流动相A为10mmol/L的乙酸铵溶液,流动相B为甲醇,色谱柱为Thermo Gold C_(18)(1.9μm,2.1 mm×50 mm)... 详细信息

建立苯磺酸氨氯地平片中苯磺酸甲酯和苯磺酸乙酯超高效液相色谱质谱的检测方法.采用梯度洗脱法同时对苯磺酸甲酯和苯磺酸乙酯进行检测,流动相A为10mmol/L的乙酸铵溶液,流动相B为甲醇,色谱柱为Thermo Gold C_(18)(1.9μm,2.1 mm×50 mm),流速为0.3.mL/min,柱温为40℃,进样量为5μL.基于苯磺酸甲酯(定量离子对173.1/155.1,定性离子对173.1/91.0)和苯磺酸乙酯(定量离子对2.4.0/159.1)优化的质谱法,方法线性良好(苯磺酸甲酯:y=1080x+467,r=0.9998.苯磺酸乙酯:y=2.7x+189,r=0.9999),苯磺酸甲酯和苯磺酸乙酯的检测精密度分别为94.0和97.3.,准确度分别为101.9%和99.3.,相对标准偏差在n=12.分别为1.8%和1.4%,在n=9时分别为4.5%和4.2.,检测限均为1 ng/mL.研究表明,建立的检测方法快速、灵敏、重现性好,可用于苯磺酸氨氯地平片中苯磺酸甲酯和苯磺酸乙酯残留量的定量检测.

关键词：苯磺酸氨氯地平片苯磺酸甲酯苯磺酸乙酯液相色谱质谱法

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Construction of bimetallic Pt-Pd/CeO_(2.-ZrO_(2.-La_(2.O_(3. catalysts with different Pt/Pd ratios and its structure-activity correlations for three-way catalytic performance

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Journal of rare Earths 2023年第6期41卷 896-904,I0004页

作者： Kai chen Jie Wan Ting Wang Qi Sun renxian Zhou Institute of Catalysis Zhejiang UniversityHangzhou 310028China Energy research Institute Nanjing Institute of TechnologyNanjing 211167China School of Environmental engineer.ng Nanjing Institute of TechnologyNanjing 211167China

A series of Pt-Pd bimetallic catalysts supported on CeO_(2.-ZrO_(2.-La_(2.O_(3. mixed oxides were synthesized through the conventional impregnation ***-way catalytic performance evaluations alo{3. with detailed physio-chemical characterizations were carried out to establish possible structure-activity *** show that on the one hand,different Pt/Pd ratios can stro{3.ly affect the TWC behaviors of Pt-Pd/CZL catalysts by modulati{3. the synergistic effect between Pt and *** the other hand,higher Pt/Pd ratio also favors better dispersion of precious *** improved precious metals(PM)dispersion can promote the metal-support interaction and increase the surface oxygen vacancies concentration,thereby raisi{3. the dynamic oxygen storage/release capacity,improvi{3. the redox ability as well as enha nci{3. the thermal stability of the Pt-Pd/CZL ***,the stro {3. metal-support interaction can augment surface oxygen vacancy concentration,thereby benefiti{3. low temperature CO and NO reaction via augmented NOxadsorption and nitrate conversion.

关键词： Three-way catalyst Bimetallic Metal-support interaction Dispersion Dynamic oxygen storage/release capacity rare earths

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