Objective Eosinophil progenitor cells(EoP)play a critical role in allergic airway inflammation in *** studies have revealed that the expression of IL-25 receptor subunits(IL-17RA and IL-17RB)increased on eosinophils(E...
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Objective Eosinophil progenitor cells(EoP)play a critical role in allergic airway inflammation in *** studies have revealed that the expression of IL-25 receptor subunits(IL-17RA and IL-17RB)increased on eosinophils(Eos)from allergic asthmatics upon allergen inhalation but few study explored the role of IL-25 on ***,in this research we examined the possible role of IL-25 on EoP in allergic asthmatics challenged by allergen,as well as in animal models where we verified the changes of newly produced Eos after IL-25 *** Asthmatics(n=14,during 2017-2018)who developed allergen-induced early and late responses were enrolled in this *** was collected at pre-and 24 h *** expression of IL-17RA and IL-17RB were evaluated by flow cytometry on *** vitro migration assay was used to examine migrational responses of EoP and hematopoietic cells(HPC)from these *** animal models,mice were grouped according to whether IL-25 was knock-out and whether mice were sensitized and challenged by ovalbumin(OVA)into asthmatic,control,knockout asthmatic and knockout control *** tissues,bronchoalveolar lavage flow(BALF)and bone marrow tissues of these mice were collected in order to evaluate airway inflammation and amount of newly produced(Brdu positive)and mature *** EoP expressing IL-17RB were significantly increased after allergen inhalation in allergic asthmatics[(514±138)vs.(1146±450)/106 cells,pre-and post-challenge,F=6.819,P=0.022].Preexposure to IL-25 primed the migrational responsiveness of EoP to stromal cell-derived factor(SDF)1α[(39.0±10.1)%vs.(73.0±7.9)%,control and IL-25 exposure groups respectively,P=0.021,95%CI 5.19%~58.45%].In OVA sensitized mice,knockout of IL-25 significantly reduced Eos and newly produced Eos percentage in the BALF[Eos,(7.8±2.0)%vs.(3.1±0.6)%,asthmatic and knockout asthmatic group respectively,P=0.002,95%CI-7.57%to-1.98%;Brdu positive Eos,(50.0±7.6)%vs.(8.6±4.3)%,asthmatic and
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