检索结果-南通市图书馆

本实验应用荧光光,圆二色性光谱和酶活力的变化系统研究了Osmolytes包括:二甲基亚砜、甘氨酸、脯氨酸和蔗糖对胍变性的免肌肌酸激酶构象和活力的影响.结果表明低浓度的二甲基亚砜(<40%)、甘氨酸(<1.5M)、脯氨酸(<2.5M)和蔗糖(<1.2M)降低了肌酸激酶去折叠和失活的速度常数,增加了去折叠和失活

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

鸡肝脂肪酸合成酶脲变形去折叠及失活

鸡肝脂肪酸合成酶脲变形去折叠及失活

引用

中国生物化学与分子生物学会第八届会员代表大会暨全国学术会议

作者：吴柏南朴龙斗周海梦田维熙清华大学生物科学与技术系教育部蛋白质科学重点实验室

本文研究了鸡肝脂肪酸合成酶[acyl-CoA:malonyl-CoA C-acyltransferase(decarboxylating,oxoacyl-andenoyl-reducing and thioester-hydrolyzing)EC 2.***.1.85]在脲溶液中的失活和构象变化.结果表明在整体构象发生明显变化之前脂肪酸合成酶的酮酯酰-CoA还原活性及乙酰乙酰-CoA还原活性完全丧失.荧光和CD的结果显示在较高浓度的脲溶液中才发生明显的构象变化.动力学分析说明失活和去折叠过程都是由快慢

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Extraction and DNA Digestion of 5'-Phosphodiesterase from Malt Root

引用

Tsinghua Science and Technology 2008年第4期13卷 480-484页

作者：邹和昌蔡光圻蔡文李海龙顾益朴龙斗孟凡国 Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China Yangtze Delta Region Institute of Tsinghua University Jiaxing 314050 China Zhejiang Jiaxing Pharmacology Corporation Jiaxing 314001 China Yangtze Delta Region Institute of Tsinghua University Jiaxing 314050 China

This study investigated the extraction of 5＇-phosphodiesterase from malt root and the degradation of nucleic acids by this enzyme. The extraction used grade precipitation with ammonium sulfate and enzymatic hydrolysis. Samples were assayed using the modified Bradford method and high performance liquid chromatography. The results show that 5＇-phosphodiesterase is isolated by grade precipitation with 30% and 80% saturation of ammonium sulfate and can be utilized to degrade deoxyribonucleic acid. The hydrolysate has four kinds of deoxynucleotides： 5＇-dCMP, 5＇-dTMP, 5＇-dAMP, and 5＇-dGMP. The optimum reaction temperature is 70℃, and the optimum pH is 5.5-6.0 for the reaction. The percentage of deoxynucleotides indicated by the China Pharmacopoeia （2000 edition） in the product is over 70%. The extraction of 5＇-phosphodiesterase from malt root is shown to be possible and economical. Products from the enzYmatic hydrolysate of DNA meet the pharmacopoeia.

关键词： 5'-phosphodiesterase malt root deoxyribonucleic acid

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Towards the computational simulation integrated inhibition kinetics and unfolding of tyrosinase by arabinose

Towards the computational simulation integrated inhibition k...

引用

第十届全国酶学学术讨论会

作者：严莉朴龙斗浙江清华长三角研究院浙江省应用酶学重点实验室

Tyrosinase inhibitors have potential applications in medicine,cosmetics and agriculture to prevent hyperpigmentation or browning *** are effective tyrosinase inhibitors and natural plant extracts. We studied the inhibitory effects of one such flavonoid,hesperetin,on mushroom tyrosinase using inhibition kinetics and computational *** reversibly inhibited tyrosinase in a competitive manner with K=4.03±0.26 *** of ANS-binding fluorescence showed that hesperetin induced the

关键词： tyrosinase inhibition kinetics hesperetin flavonoid docking simulation

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

耐热无机焦磷酸酶的表达纯化与性质研究

耐热无机焦磷酸酶的表达纯化与性质研究

引用

第九届全国酶学学术讨论会暨邹承鲁诞辰85周年纪念会

作者：牟航夏勇吴学强朴龙斗孟凡国周海梦清华大学生物科学与技术系浙江清华长三角研究院山东农业大学生命科学学院

无机焦磷酸酶(EC 3.***.1.1,PPase)广泛地存在于各种类型的细胞内,参与催化多种代谢途径中的焦磷酸水解生成正磷酸的反应。PPase 在氧化磷酸化、氨基酸和脂肪酸活化、辅酶合成、DNA 和 RNA 聚合等反应过程中均起着重

关键词：无机焦磷酸酶嗜热栖热菌克隆表达纯化与性质

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Characterization of the Partially Folded Monomeric Intermediate of Creatine Kinase

引用

Tsinghua Science and Technology 2002年第4期7卷 347-351页

作者：朴龙斗周海梦 Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

The importance of understanding the protein folding pathway and intermediates is well recognized on the basis of extensive studies of protein folding in vitro and in vivo. Creatine kinase (CK) is a typical model for studying unfolding and refolding of proteins due to several interesting properties. Recent studies on the folding of CK show that its partially folded monomeric intermediate is present kinetically and is stable at equilibrium. The present paper contains 33 references as a mini review to characterize the properties of CK from studies on the CK folding pathway. Characterization of these intermediates is an essential step toward understanding the mechanism of protein folding. Some well determined schemes are suggested as protein folding models.

关键词： creatine kinase intermediate partially folded state folding pathway

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Computational simulations integrating inhibition kinetics of tyrosinase by oxalic acid

Computational simulations integrating inhibition kinetics of...

引用

第十届全国酶学学术讨论会

作者：严莉朴龙斗周海梦浙江清华长三角研究院浙江省应用酶学重点实验室

Tyrosinase inhibition studies are important for agricultural and medicinal *** predictions and enzymatic assays via kinetics may be used to detect effective inhibitors of *** predicted the 3D structure of tyrosinase from Agaricus bisporus,used a docking algorithm to simulate binding between tyrosinase and oxalic acid(OA),and studied the reversible inhibition oftyrosinase by *** were successful(binding energies for Dock6.3=-18.76 and AutoDock4.2=-2.47 kcal/mol),suggesting that

关键词： tyrosinase oxalic acid docking simulation inhibition kinetics mixed-type carboxyl group

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Activation of Calf Intestinal Alkaline Phosphatase by Trifluoroethanol

引用

Tsinghua Science and Technology 2001年第5期6卷 426-431,457页

作者：曹志方徐真朴龙斗周海梦 Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100 10 1 China Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

Alkaline phosphatase is a stable enzyme which is strongly resistant to urea, guanidine hydrochloride, acid pH, and heat. But there have been few studies on the effect of organic cosolvents on the activity and structure of alkaline phosphatase. The activity of calf intestinal alkaline phosphatase (CIAP) is markedly increased when incubated in solutions with elevated trifluoroethanol (TFE) concentrations. The activation is a time dependent course. There is a very fast phase in the activation kinetics in the mixing dead time (30 s) using convential methods. Further activation after the very fast phase follows biphasic kinetics. The structural basis of the activation has been monitored by intrinsic fluorescence and far ultraviolet circular dichroism. TFE (060%) did not lead to any significant change in the intrinsic fluorescence emission maximum, indicating no significant change in the tertiary structure of CIAP. But TFE did significantly change the secondary structure of CIAP, especially increasing α helix content. We conclude that the activation of CIAP is due to its secondary structural change. The time for the secondary structure change induced by TFE preceds that of the activity increase. These results suggest that a rapid conformational change of CIAP induced by TFE results in the enhancement of CIAP activity, followed by further increase of this activity because of the further slightly slower rearrangements of the activated conformation. It is concluded that the higher catalytic activity of CIAP can be attained with various secondary structures.

关键词： alkaline phosphatase trifluoroethanol activation conformation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

High-throughput integrated analyses for the tyrosinase-induced melanogenesis:microarray,proteomics and interactomics studies

High-throughput integrated analyses for the tyrosinase-induc...

引用

第十届全国酶学学术讨论会

作者：吕志荣朴龙斗周海梦浙江清华长三角研究院浙江省应用酶学重点实验室

The tyrosinase gene was overexpressed in HEK293 cells,and then a DNA microarray and proteomic tools were applied to detect the dysregulated genes in highly pigmented *** candidate genes from the microarray were compared to the yeast two-hybridization *** prediction via protein-protein interaction mapping suggested the existence of 66 hub genes in *** importantly,RNA binding

关键词： Tyrosinase Melanogenesis Microarray Proteomics Interactomics

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：