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天然水中氯苯、多氯联苯、多环芳烃和邻苯二甲酸酯的分析

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环境化学 1987年第5期 52-58页

作者：康君行,hing-biu lee 中国科学院生态环境研究中心 National Water Research Institute Canada Centre for Inland Waters

本工作建立了天然水中氯苯、多氯联苯、多环芳烃和邻苯二甲酸酯的多残留分析方法。在中性pH条件下,用二氯甲烷萃取水样,提取液经硅胶柱层析后,分成三个级份;第一级份含有氯苯和多氯联苯;多环芳烃存在于第二级份;第三级份内为邻苯二甲酸... 详细信息

本工作建立了天然水中氯苯、多氯联苯、多环芳烃和邻苯二甲酸酯的多残留分析方法。在中性pH条件下,用二氯甲烷萃取水样,提取液经硅胶柱层析后,分成三个级份;第一级份含有氯苯和多氯联苯;多环芳烃存在于第二级份;第三级份内为邻苯二甲酸酯。用毛细管柱ECD/GC和FID/GC测定所得到的三个级份,在选择离子监测方式的GC/MSD上做进一步的鉴定。

关键词：邻苯二甲酸酯多氯联苯多环芳烃氯代烃

Blastocyst-like embryo surrogates from stem cells in implantation models

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Reproductive and Developmental Medicine 2022年第4期6卷 225-233页

作者： Siyu Tian Hanzhang Ruan William Shu-biu Yeung Yin Lau lee Department of Obstetrics and Gynaecology Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong SAR 999077China Shenzhen Key Laboratory of Fertility Regulation Reproductive Medicine CenterThe University of Hong Kong-Shenzhen HospitalShenzhen 518053China Centre for Translational Stem Cell Biology Building 17WThe Hong Kong Science and Technology ParkHong Kong 999077China

Successful embryo implantation requires highly coordinated maternal-embryo *** failure is a major factor contributing to ***,the mechanism underlying implantation failure remains *** improved understanding of the early implantation process not only improves the success rate of assisted reproductive treatments but also helps in studying the pathophysiology of reproductive *** to ethical concerns,in vivo studies of human embryo implantation are not ***,the results obtained from animal models cannot be directly applied to *** the years,in vitro implantation models have been developed to investigate implantation *** this review,we discuss the use of different models for generating embryo-like surrogates to study early embryo development and implantation in vitro,with a specific focus on stem cell-derived blastocyst-like embryo *** is no definitive evidence that the recently established embryo-like models re-capitulate all developmental events of human embryos during the peri-implantation ***,stem cell-derived embryo surrogates are the most valuable tools for studying the mechanisms of early cell lineage differentiation and developmental failures during implantation.

关键词： implantation models Stem cells Embryo surrogates Endometrium Trophoblastic spheroid Blastoids

Use of biological and chemical molecules in regulating embryo implantation and endometrial receptivity

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Reproductive and Developmental Medicine 2022年第4期6卷 234-242页

作者： Xian Chen Shu-Ya Sun Ernest Hung-Yu Ng Raymond Hang-Wun Li William Shu-biu Yeung Kai-Fai lee Department of Obstetrics and Gynaecology LKS Faculty of Medicinethe University of Hong KongHong Kong SARChina Shenzhen Key Laboratory of Fertility Regulation the University of Hong Kong-Shenzhen HospitalShenzhen 518053China

This review summarizes the drugs and chemicals that may modulate embryo ***-hormonal molecules,including aspirin,improved endometrial blood flow,while low molecular weight heparin,vitamin E,sildenafil,and atosiban modulated the expression of endometrial *** factors,including human chorionic gonadotropin and growth hormones,can regulate the expression of endometrial receptivity *** immunomodulatory molecules,including granulocyte colony-stimulating factor,peripheral blood mononuclear cells,autologous platelet-rich plasma,and intralipid and intravenous immunoglobulins,may improve implantation rate by modulating endometrial immune *** extracts of the Chinese herbs Paeonia lactiflora and Perilla frutescens increased the expression of leukemia inhibitory factors in endometrial epithelial ***,the use of the commercially available Library of Pharmacologically Active Compounds with a high-throughput screening method has provided an approach to screen for compounds that may potentially enhance or suppress embryo *** these biomedical findings translate into clinical effects that enhance or suppress embryo implantation requires further investigation.

关键词： Embryo implantation Endometrial receptivity fertilization Repeated implantation failure Birth control Emergency contraception

Human embryonic stem cells as an in vitro model for studying developmental origins of type 2 diabetes

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World Journal of Stem Cells 2020年第8期12卷 761-775页

作者： Andy Chun-Hang Chen Kai Fai lee William Shu biu Yeung Yin Lau lee Department of Obstetrics and Gynaecology The University of Hong KongHong KongChina Shenzhen Key Laboratory of Fertility Regulation The University of Hong Kong Shenzhen HospitalShenzhen 518053Guangdong ProvinceChina

The developmental origins of health and diseases(DOHaD)is a concept stating that adverse intrauterine environments contribute to the health risks of *** the theory emerged more than 30 years ago,many epidemiological and animal studies have confirmed that in utero exposure to environmental insults,including hyperglycemia and chemicals,increased the risk of developing noncommunicable diseases(NCDs).These NCDs include metabolic syndrome,type 2 diabetes,and complications such as diabetic *** the effects of different environmental insults on early embryo development would aid in understanding the underlying mechanisms by which these insults promote NCD *** stem cells(ESCs)have also been utilized by researchers to study the *** have pluripotent characteristics and can be differentiated into almost every cell lineage;therefore,they are excellent in vitro models for studying early developmental *** importantly,human ESCs(hESCs)are the best alternative to human embryos for research because of ethical *** this review,we will discuss different maternal conditions associated with DOHaD,focusing on the complications of maternal ***,we will review the differentiation protocols developed to generate different cell lineages from ***,we will review how hESCs are utilized as a model for research into the *** effects of environmental insults on hESC differentiation and the possible involvement of epigenetic regulation will be discussed.

关键词： Development origins of health and diseases Maternal diabetes Environmental insults Type 2 diabetes Human embryonic stem cells Epigenetics

超临界二氧化碳萃取—GC/ECD测定土壤中高级脂肪酸

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福建分析测试 1996年第1期5卷 397-400,424页

作者：翁建华黄连芬 Hihg-biu lee T.E.Peart 中国水利水电科学研究院北京100044 国家水研究所

本文报导了一种用超临介二氧化碳萃取—气相色谱电子捕获检测器测定土壤中高级脂肪酸的实用分析方法.即采用超临介二氧化碳萃取法萃取土壤中的高级有机酸,选用五氟苄基溴(PFBBr)衍生化反应生成相应的酯,经过硅胶柱纯化、最后用GC(ECD)... 详细信息

本文报导了一种用超临介二氧化碳萃取—气相色谱电子捕获检测器测定土壤中高级脂肪酸的实用分析方法.即采用超临介二氧化碳萃取法萃取土壤中的高级有机酸,选用五氟苄基溴(PFBBr)衍生化反应生成相应的酯,经过硅胶柱纯化、最后用GC(ECD)进行测定。方法简便、可靠。应用该方法测定了中国几类未开垦森林土壤中的高级脂肪酸,获得了满意结果。

关键词：超临界二氧化碳萃取气相色谱电子捕获检测器高级脂肪酸衍生化五氟苄基溴

人输卵管胚胎营养因子对着床前鼠胚胎基因表达的影响

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中华妇产科杂志 2007年第9期42卷 605-607页

作者：姚元庆李启辉许嘉森何柏松杨树标第四军医大学唐都医院妇产科香港大学玛丽医院妇产科

目的探讨人输卵管上皮细胞分泌的胚胎营养因子(ETF)对着床前鼠胚胎基因表达的影响。方法采用液相色谱技术从体外培养的人输卵管上皮细胞系 OE-E6/E7的培养液中分离出ETF,加入培养液后培养鼠胚胎。采用差异显示 RT-PCR 技术比较 ETF 培... 详细信息

目的探讨人输卵管上皮细胞分泌的胚胎营养因子(ETF)对着床前鼠胚胎基因表达的影响。方法采用液相色谱技术从体外培养的人输卵管上皮细胞系 OE-E6/E7的培养液中分离出ETF,加入培养液后培养鼠胚胎。采用差异显示 RT-PCR 技术比较 ETF 培养的鼠胚胎(培养组)与普通培养液(未加入 ETF)培养的鼠胚胎(对照组)间的基因表达差异。将差异表达片段分离后,进行扩增、克隆、测序和分析。结果培养组胚胎与对照组胚胎共分离克隆了8个差异表达基因,即:RABgeranylgeranyl 转移酶、DEAD-box 蛋白50、RNA 结合结构域蛋白27、二磷酸甲羟戊酸盐脱羧酶、丝氨酸/精氨酸重复基质1、肿瘤差异表达基因2(TDE2)、T 细胞受体α/δ、胚胎发育因子1。结论人输卵管上皮细胞分泌的 ETF 对着床前鼠胚胎的基因表达有影响。

关键词：胚胎营养因子植入前期胚胎基因表达基因表达谱逆转录聚合酶链反应

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低浓度磷酸一酯的膜富集和气相色谱测定

水文 1999年第4期18卷 19-21页

作者：翁建华 hing-biulee 田世忠黄连芬刘晓茹中国水利水电科学研究院北京100044 加拿大国家水研究所武汉大学环科系武汉430072

磷酸一酯（RO）P（O）（OH）2，是一种强权性的有机磷化合物。研究了水体中磷酸一（1-萘）酯的富集和气相色谱的分析，建立了一个简便快速、高灵敏度、实用的分析方法。富集是采用固相萃取方法，选用载有十八烷基硅烷的尼龙滤膜吸附，... 详细信息

磷酸一酯（RO）P（O）（OH）2，是一种强权性的有机磷化合物。研究了水体中磷酸一（1-萘）酯的富集和气相色谱的分析，建立了一个简便快速、高灵敏度、实用的分析方法。富集是采用固相萃取方法，选用载有十八烷基硅烷的尼龙滤膜吸附，甲醇洗脱，回收率达72％-78％。它的气相色谱分析选用BSTFA为衍生剂，反应条件为温度65℃，反应时间30min，获得了满意的结果。方法的最低检测限为0．06ng。

关键词：磷酸一酯膜富集气相色谱衍生化

Function of Lin28a/Wnt/β-catenin in Regulating Premature Ovarian Failure

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Function of Lin28a/Wnt/β-catenin in Regulating Premature Ov...

An update on glycodelin research-its implication in reproductive medicine

中国生理学会生殖科学专业委员会—中国动物学会生殖生物学...

作者： Jing Chen Weimin Liu Kai Fai lee Shu biu Yeung Department of Obestrics and Gynaecology Li Kai Shing Faculty of MedicineThe University of Hong Kong Institute of Shenzhen University of Hong Kong

＜正＞Infertility is a prevalence of disease that besets 15%of couples in childbearing age around the *** ovarian failure（POF）,defined as primary ovarian defection and characterized as cessation of menstruation or absent function of ovarian follicles in women under the age of 40,accounts for approximately 1%of infertility disease in women at childbearing *** study on the

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An update on glycodelin research-its implication in reproduc...