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医学影像学杂志 2003年第5期13卷 314-314页

作者：孟淑春任广成山东省聊城市第三人民医院放射科山东聊城252000

患者女性,12岁.外伤后右膝关节持续性钝痛6天.查体:右膝关节皮肤光滑,无红、肿、热及功能障碍.

关键词：蜡泪样骨病 X线诊断

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农业部蔬菜种子质量检验机构获国际认证

农家参谋(种业大观) 2013年第3期 30-30页

作者：孟淑春姜宝泉

挂靠于北京市农林科学院蔬菜研究中心的农业部蔬菜种子质量监督检验测试中心,近日获得ISTA执行委员会的确认和授权,正式成为ISTA国际认可实验室。该检验测试中心亦是我国目前唯一通过ISTA认可,可以授权出具种子质量进出口国际检验证书,... 详细信息

挂靠于北京市农林科学院蔬菜研究中心的农业部蔬菜种子质量监督检验测试中心,近日获得ISTA执行委员会的确认和授权,正式成为ISTA国际认可实验室。该检验测试中心亦是我国目前唯一通过ISTA认可,可以授权出具种子质量进出口国际检验证书,检验数据国际对等互认的第三方种子质量检验机构。获得ISTA国际实验室认可后,农业部蔬菜种子

关键词：蔬菜种子质量监督检验行政管理部门农业部

Comparison of Methods for Ultra-drying of Chinese Chive Seeds

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Agricultural Science & Technology 2013年第4期14卷 598-601页

作者：孟淑春李秀清马连平刘玲北京市农林科学院蔬菜研究中心农业部华北地区园艺作物生物学与种质创制重点实验室农业部都市农业(北方)重点实验室北京100097

[Objective] This study aimed to identify the ultra-drying method most suitable for the storage of Chinese chive seeds through comparing different ultra-drying meth- ods. [Method] Through setting treatments including freezing, silica gel drying and heating drying at 50℃ as well as two controls, the effects of different drying methods for storing ultra-dried Chinese chive seeds were compared. [Result] There were no significant differences in the germination rate and seedling length between Chinese chive seeds treated with heating drying at 50℃ and those treated with either freezing or get drying at the initial stage of the storage. Storing of Chinese chive seeds previously treated with either heating drying at 50℃, or freezing or gel drying for 0-98 months at 20℃ hadn＇t caused any adverse effect on the germination rate of seeds. And the effect of different storage conditions on seedling length displayed earlier than that on the germination rate. There was no significant difference on seedling length between seeds treated with heating drying at 50℃ and those treated with freezing drying after stored for as long as 98 months, revealing similar efficacy on storing seeds. [Conclusion] Freezing drying requires huge investment and cost for perennial running, and silica gel drying is slow in drying and time-consuming. By contrast, heating drying at 50℃ is an economic and simple method, which can enable the Chinese chive seeds to sustain a longevity of eight years or more.

关键词： Chinese chive seeds Ultra-drying methods Seed longevity

Seed Germination of Eggplant (Solanum melongena L.) Luba 2 Treated with Hydrogen Peroxide

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Agricultural Science & Technology 2014年第7期15卷 1117-1119页

作者：孟淑春崔彦玲邢宝田刘玲北京市农林科学院蔬菜研究中心/农业部华北地区园艺作物生物学与种质创制重点实验室/农业部都市农业(北方)重点实验室农业部/北京蔬菜种子监督检验测试中心北京100097

[Objective] This study aimed to investigate the effect of hydrogen peroxide on the seed germination of eggplant （Solanum melongena L.） Luba 2.[Method] The seeds of Luba 2 were separately soaked in 20％,40％,60％ and 80％ hydrogen peroxide solution for 2,5 and 10 min before seed *** germination rate and days to reach the maximum germination rate were calculated.[Result] Treatment with low concentrations （20％） of hydrogen peroxide for 2 and 5 min made eggplant Luba 2 germinate in advance,and shortened the time to reach the maximum germination *** with high concentrations （60％ and 80％） of hydrogen peroxide solution for longer time （10 min） caused significant damage to the seeds,delayed seed germination,and reduced the germination rate.[Conclusion] This study will provide theoretical references for the production practices of eggplant.

关键词： Hydrogen peroxide Eggplant seeds Seed germination

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囊性脑膜瘤误诊为硬膜下血肿两例报告

中外医用放射技术 2001年第12期20卷 72-72页

作者：黄贤会孟淑春等山东聊城市东昌医院252000

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黄瓜新品种京研冬美9号的SSR纯度鉴定

中国瓜菜 2023年第6期36卷 23-27页

作者：孟淑春刘立功宋晓玉宋顺华张峰蔬菜生物育种全国重点实验室/国家蔬菜工程技术研究中心·农业农村部蔬菜种子质量监督检验测试中心·北京市农林科学院蔬菜研究所北京100097 安徽农业大学园艺学院合肥230036

种子纯度鉴定是保证种子质量的关键环节。田间小区种植鉴定法,周期长、费用高,无法满足种子贸易中快速鉴定的要求,且受环境影响大,结果不够准确。DNA电泳鉴定技术为种子的纯度鉴定提供了一种更为快速、高效的方法。笔者采用72对SSR引物... 详细信息

种子纯度鉴定是保证种子质量的关键环节。田间小区种植鉴定法,周期长、费用高,无法满足种子贸易中快速鉴定的要求,且受环境影响大,结果不够准确。DNA电泳鉴定技术为种子的纯度鉴定提供了一种更为快速、高效的方法。笔者采用72对SSR引物对黄瓜品种京研冬美9号及其亲本进行多态性筛选。结果表明,12对引物在亲本间具有多态性,并都表现出共显性,在杂交后代中表现为清晰、稳定的父母本互补的带型,特异性强,可以用来区别其中混杂的母本、父本及其他品种的种子。这12对SSR引物作为京研冬美9号及其亲本的核心引物,构成的京研冬美9号的SSR指纹图谱,为京研冬美9号杂交种的亲本提纯、纯度检测及真实性鉴定等工作提供了有效的技术支持。其中,2对引物SSR17922和SSR02118特异性强且谱带清晰,利用这2对引物对京研冬美9号黄瓜种子进行检测,鉴定结果与田间鉴定结果一致,被选为鉴定黄瓜京研冬美9号纯度的特异引物。

关键词：黄瓜京研冬美9号 SSR 纯度鉴定

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大棚越夏菠菜栽培技术

西北园艺（蔬菜） 2007年第3期 19-19页

作者：孟淑春刘明池国家蔬菜系统工程技术研究中心

菠菜耐寒不耐热，一般品种生长期间当气温超过25℃时就会生长不良，品质差。如果在夏季利用休闲期大棚进行遮阴防雨，并选用耐热品种及配套的栽培技术，于6～7月份播种，7～9月份即可上市，既解决了菠菜炎夏淡季的供应，种植者经济收益... 详细信息

菠菜耐寒不耐热，一般品种生长期间当气温超过25℃时就会生长不良，品质差。如果在夏季利用休闲期大棚进行遮阴防雨，并选用耐热品种及配套的栽培技术，于6～7月份播种，7～9月份即可上市，既解决了菠菜炎夏淡季的供应，种植者经济收益也颇丰。

关键词：夏菠菜技术栽培大棚耐热品种生长不良经济收益生长期

Effects of Ultra-drying Treatment on Large Amounts of Four Kinds of Crop Seeds

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Agricultural Science & Technology 2009年第2期10卷 82-85,89页

作者：孟淑春张海英刘庞源何伟明国家蔬菜系统工程技术研究中心

This study was to understand the physiological changes of four kinds of crop seeds including baby corn, cowpea, radish and rape- seed, under the treatment of ultra-drying. Large amounts of seeds as experimental materials （ 10 kg for each material） were respectively dried to the suitable water content consistent with the ultra-dried seed criterion via heating at 50 ~C; then the physiological indices of these ultra-dried seeds such as water content, seed germination, dehydrogenase activity and SOD activity were measured. The results showed that except cowpea seeds, whose ultra-drying treatment should be controlled between two and four days, other three kinds of seeds were tolerant to high tem- perature and low water content. The ultra-dried seeds assumed similar or even higher activities than CKs, but showed no damage symptom. Our result proves that ultra-drying treatment via heating at 50 ℃ is safe and efficient to experimental seeds.

关键词： Large amounts of seeds Ultra-drying treatment via heating Water content Seed vigor

Studies on Genomic DNA Extraction and Establishment of AFLP Reaction System in Chinese Cabbage

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Agricultural Science & Technology 2009年第3期10卷 29-35页

作者：孟淑春张海英郑晓鹰刘玉梅王永健国家蔬菜系统工程技术研究中心北京100097 中国农业科学院蔬菜花卉研究所北京100081

[Objective] The obtained clear AFLP fingerprint of Chinese cabbage provided basis for studies on the molecular markers of Chinese cabbage cultivars and the phylogenetic relationship among Chinese cabbage cultivars. [Method] With the test materials of leaves of Chinese cabbages, the high-quality total DNA from leaves of Chinese cabbages was extracted by the modified CTAB method. DNA restriction-ligase reaction, pre-amplification and selective amplification were optimized, and the AFLP silver-staining reaction system for Chinese cabbage was established. [Result] The quality of DNA template influenced restriction enzyme digestion and the subsequent ligase amplification reaction, while the modified CTAB extraction method could be used in AFLP analysis of Chinese cabbage to obtain a clear AFLP fingerprint. The optimum conditions for restriction enzyme digestion of genomic DNA from Chinese cabbage were as follows: 150 g DNA template, 12.5 μl reaction volume, 1.25 U Eco R Ⅰ, 1.25 U Mse Ⅰ and 5×Reaction Buffer with 4 h at 37 ℃. The ligation reaction with 2.5 h at 20 ℃ was the optimum condition. Six pairs of primers including E-AAC/M-CAG, E-AAG/M-CAC, E-ACA/M-CTG, E-ACT/M-CAC, E-ACT/M-CTT and E-ACT/M-CTC all had its own stable and clear patterns. [Conclusion] With abundant bands and high polymorphism, AFLP selective amplification is an efficient molecular marker for genomic polymorphism of Chinese cabbage.

关键词： Chinese cabbage DNA extraction AFLP

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京研绿翡翠黄瓜品种纯度的SSR鉴定

湖北农业科学 2022年第1期61卷 98-101页

作者：孟淑春徐秀苹刘立功宋顺华张峰北京市农林科学院蔬菜研究中心/农业农村部华北地区园艺作物生物学与种质创制重点实验室/农业农村部都市农业(北方)重点实验室北京100097 中国科学院植物研究所系统与进化植物学国家重点实验室北京100093

为了确保种子遗传质量,准确高效地鉴定杂交黄瓜种子的品种纯度,用63对SSR引物对黄瓜(Cucumis sativus L.)品种京研绿翡翠及其亲本进行多态性筛选。结果表明,16对引物在亲本间具有多态性,并都表现出共显性,在杂交后代中表现为清晰、稳定... 详细信息

为了确保种子遗传质量,准确高效地鉴定杂交黄瓜种子的品种纯度,用63对SSR引物对黄瓜(Cucumis sativus L.)品种京研绿翡翠及其亲本进行多态性筛选。结果表明,16对引物在亲本间具有多态性,并都表现出共显性,在杂交后代中表现为清晰、稳定的父母本互补的带型,特异性强,可以用来区别其中混杂的母本、父本及其他品种的种子。这16对SSR引物作为京研绿翡翠及其亲本的核心引物,构成京研绿翡翠的SSR指纹图谱,为京研绿翡翠杂交种的真伪鉴定、纯度检测及亲本提纯等工作提供准确的技术信息。其中10对引物扩增的条带可分辨程度高,利用这10对引物对京研绿翡翠黄瓜种子进行检测,其鉴定结果与田间鉴定结果一致。

关键词：京研绿翡翠黄瓜 SSR 纯度鉴定

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