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Role of testosterone in NO/EDRF-induced relaxation of the rabbit aorta

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Journal of Medical Colleges of PLA(China) 1996年第1期11卷 27-29页

作者：周可祥吴赛珠沈洁翁心植

To determine whether the therapeutic effectiveness of testosterone in male elderly coronary heart disease patients is related to the production of nitric oxide (NO), which accounts for the biological activity of endothelium derived relaxing factor (EDRF), we studied the regulatory effect of testosterone on production of NO/EDRF in the aorta and cultured vascular endothelial cells. The production of NO/EDRF was detected by the stimulation of cGMP formation in rabbit fetal lung-6 cells. The results showed that testosterone stimulated the production of NO/EDRF in both the aorta and cultured vascular endothelial cells, suggesting that NO/EDRF-dependent vasodilation is one of the mechanism by which testosterone alleviates angina pectoris and improves myocardial ischemia in elderly male coronary heart disease patients.

关键词： testosterone endothelium derived relaxing factor epithelium nitric oxide

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去卵巢大鼠动脉壁中雌激素受体的表达及意义

中华核医学与分子影像杂志 2000年第6期20卷 40-40页

作者：吴赛珠阮云军孟素荣周可祥张喜安翁心植郭志刚彭健第一军医大学南方医院心内科第一军医大学南方医院病理科首都医科大学附属北京红十字朝阳医院

雄激素对老龄大鼠血管平滑肌组织雄激素受体及血小板源性生长因子受体基因表达的影响

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中华老年医学杂志 2004年第5期23卷 303-303页

作者：吴赛珠吕红松吴迎星周可祥孙飞马瑞郑华刘长青第一军医大学南方医院心血管内科广州市510515

2001年10月至2003年2月,我们通过本试验探讨不同剂量的庚酸睾酮(TE) 对衰老雄性大鼠血管平滑肌组织中雄激素受体(AR) 及血小板源性生长因子受体-β(PDGFR-β) 基因表达的影响。

关键词：雄激素老龄大鼠血管平滑肌雄激素受体血小板源性生长因子受体基因表达心血管疾病

Sex Hormones Affect Aging Process by Influencing Lipid Profiles, Cellular Immunological Function and Lipid Peroxides and Oxidation System

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South China Journal of Cardiology 2003年第2期4卷 97-104页

作者：吴赛珠谭家余周忠江周可祥容志毅 Dept.of Cardiology Nanfang Hospital First Military Medical University Guangzhou 510515 P. R. China Dept.of Cardiology Nanfang Hospital First Military Medical University Guangzhou 510515 P. R. Chinabjectives To investigate the correlation between sex hormones(SH) and aging. Methods Through epidemiological investigation in our country the levels of SH were measured by radioimmunoassy lipid profile glucose and apolipoprotein by automatic biochemic analytical instrument T cell subsets by flow cytometer and MDA SOD were evaluated by the thiobarbituric acid (TBA) test and the nitrite method modified by Oyanagui respectively using spectrophotometry. Results In men the serum levels of follicle stimulating hormone ( FSH)luteinizing hormone(LH) increased significantly with aging but serum prolactin(PRL) and progesterone(P) levels remained unchanged in all life Both testosterone (T) and free testosterone (FT) all decreased greatly with aging but 17β- estradiol( 17β- E_2) was reverse E_2 was negatively correlated with T and E_2/T increased with aging. The level of serum total cholesterol (TC) increased with aging but triglycerides (TG) remain unchanged compared with young group high - density lipoprotein cholesterol ( HDL - C) HDL - C/TC of other groups decreased significantly but low - density lipoprotein cholesterol ( LDL - C ) changed inversely HDL- C/LDL- C reduced slightly with aging and showed no difference between groups. Apolipoprotein A1 (apoA1) and apoB all enhanced greatly with aging meanwhile the ratio of apoA1/apoB decreased. The concentration of serum glucose (GLU) was unchanged in all life. To compare with those in the young group CD3 + CD4 + in other groups reduced greatly CD4 + remained unchanged. Meanwhile CD8 + increased significantly with aging. Compared with the young group serum malondialdehyde(MDA) value of the old ones increased obviously but the activity of superoxide dismutase(SOD) was reverse. By partial correlation analysis (controlling BMI FSH LH and PRL) TCLDL-CapoA

Objectives To investigate the correlation between sex hormones(SH) and aging. Methods Through epidemiological investigation in our country, the levels of SH were measured by radioimmunoassy; lipid profile, glucose and apolipoprotein by automatic biochemic analytical instrument; T cell subsets by flow cytometer; and MDA, SOD were evaluated by the thiobarbituric acid (TBA) test and the nitrite method modified by Oyanagui respectively using spectrophotometry. Results In men, the serum levels of follicle stimulating hormone ( FSH)、luteinizing hormone(LH) increased significantly with aging, but serum prolactin(PRL) and progesterone(P) levels remained unchanged in all life; Both testosterone (T) and free testosterone (FT) all decreased greatly with aging, but 17β - estradiol( 17β - E_2) was reverse ; E_2 was negatively correlated with T and E_2/T increased with aging. The level of serum total cholesterol (TC) increased with aging, but triglycerides (TG) remain unchanged; compared with young group, high - density lipoprotein cholesterol ( HDL - C) ; HDL - C/TC of other groups decreased significantly, but low - density lipoprotein cholesterol ( LDL - C ) changed inversely; HDL- C/LDL- C reduced slightly with aging and showed no difference between groups. Apolipoprotein A1 (apoA1) and apoB all enhanced greatly with aging; meanwhile the ratio of apoA1/apoB decreased. The concentration of serum glucose (GLU) was unchanged in all life. To compare with those in the young group, CD3 + , CD4 + in other groups reduced greatly, CD4 + remained unchanged. Meanwhile, CD8 + increased significantly with aging. Compared with the young group, serum malondialdehyde(MDA) value of the old ones increased obviously, but the activity of superoxide dismutase(SOD) was reverse. By partial correlation analysis (controlling BMI, FSH, LH and PRL), TC、 LDL-C、apoA1、apoB、CD8 + 、MDA of men all presented a positive correlation with E_2/T respectively, their correlation coefficients (γ) were 0. 262、0. 136、

关键词： aged man sex hormones lipid profile T lymphocyte subpopulations malondialdehyde superoxide dismutase epidemiological study

The Expression of Estrogen Receptor is Dependent on the Estrogen Level and Associated with Cholesterol - Rich Diet in Female Rat's Heart and Vascular Endothelial Cells

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South China Journal of Cardiology 2003年第1期4卷 37-42页

作者：吴赛珠刘建国周可祥刘长青马瑞孙飞隗和明 Department of Cardiology Nanfang Hospital Guangzhou 510515 P R China Atherosclerosis And Vascular Biology (AVB) Lab. Johns Hopkins Singapore Pte Ltd

Objective To study the effects of estrogen level and cholesterol - rich diet on the expression of estrogen receptor (ER) in cardiovascular tissues including vascular endothelial cells (VEC) of female rats. Methods The receptor binding assay (RBA) was adopted to measure the estrogen receptor level in aortic wall, heart and vascular endothelial cells of female rats on a cholesterol - rich diet. A ra-dioimmunoassay was employed to measure the level of serum estradiol. Results The number of ER significantly decreased in hearts, aorta and vascular endothelial cells in the ovariectomized rats and the rats on a cholesterol - rich diet. In contrast, the administration of estrogen somewhat restored the expression of ER. Conclusions For female rats, the level of estrogen affects the expression of ER in cardiovascular system. The number of ER decreases along with the decrease in the level of estrogen. A cholesterol - rich diet also can decrease the expression of ER in cardiovascular system of female rats.

关键词： Aorta Estrogen receptor Heart Vascular endothelial cell

Up and Down Expression of Androgen Receptor, Estrogen Receptor beta and Platelet Derived Growth Factor beta by Testosterone in Aortic Vascular Smooth Muscle Tissues

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South China Journal of Cardiology 2004年第2期5卷 116-122页

作者：吴赛珠吕红松周可祥孙飞马瑞郑华隗和明容志毅 Department of Cardiology Nanfang Hospital Guangzhou 510515 PR China Atherosclerosis & Vascular Biology Lab Johns Hopkins. Singapore

Objectives To investigate the effects of testosterone enanthate (TE) on serum lip- ids and lipoproteins metabolism and the expression of androgen receptor (AR) , estrogen receptor beta ( ER - β) and platelet derived growth factor beta ( PDGFR - β) in aortic vascular smooth muscle tissues (VSMTs). Methods Forty aged male rats were ran- domly divided into 4 groups, group A (placebo group) , group B (2. 5 mg/kg intramuscular injection of TE once a week ) , group C (5.0 mg/kg intramuscular injection of TE once a week ) , group D ( 10. 0 mg/kg intramus- cular injection of TE once a week). All animals were fed freely during 16 - week treatment periods. The ex- pression of AR ,ER - βand PDGFR - β were studied by Western bolt. Results Average serum LDL - C was lower in group D than that in group A ( p < 0. 01 ). Compared with the other groups, average serum TC was also lower in group D (p <0. 05). AR expression in aortic vascular smooth muscle tissues could be regulated by TE: 99.50 ±21.74, 125.38 ±28.68 and 101.98 ± 15.42 for TE concentrations at 2.5 mg/kg, 5.0 mg/kg and 10.0 mg/kg, respectively , the expression of ER - β could be regulated by TE: 92. 34 ± 18. 68, 47. 72 ± 18.12, 82.13 ±23.50, and the expression of PDGFR - β could be regulated as well by TE: 219.70 ±45. 59, 50.16 ±9. 72, 125.36 ±15. 74(Data for AR ,ER-β and PDGFR - β protein band intensity were expressed with x ± s, with control group taken as 100 ).Conclusions This study indicates that androgens have significant effects on serum lipids and lipoprotein metabolism. Testosterone enanthate at 5. 0 mg/kg can stimulate the expression of AR, but inhibite the expres- sion of PDGFR. Testosterone enanthate at the concen- trations of 5. 0 mg/kg and 10. 0 mg/kg can inhibite the expression of ER - β.

关键词： androgen receptor platelet de- rived growth factor estrogen coronary heart dis- ease aortic vascular smooth muscle testosterone

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应用细胞工程选育早熟优质晚粳85—1

安徽农业科学 1992年第1期20卷 10-12,16页

作者：曾海富张盘娣杨文保皮桃花曹效参周可祥李立明胡先国赵前进王维生朱凤金黄典平张瑞虎陈家金安徽省农科院水稻研究所无为县种子公司当涂县农业技术推广站六安县九十铺乡墩子湾村

将日本晴与黎花17杂种第二代植株的适宜时期的花药,采用细胞工程技术培育而成的85—1新品系,自1986年起经过9个县(市)12个试验、示范点作单、双晚种植,结果表明:株形优良,生长青秀,光照姿态好,后期转色佳;根系健壮发达、不早衰;穗大粒多... 详细信息

将日本晴与黎花17杂种第二代植株的适宜时期的花药,采用细胞工程技术培育而成的85—1新品系,自1986年起经过9个县(市)12个试验、示范点作单、双晚种植,结果表明:株形优良,生长青秀,光照姿态好,后期转色佳;根系健壮发达、不早衰;穗大粒多,结实率在95%左右,米质优;熟期整齐,熟相好;全生育期108—120天,平均亩产350—400公斤;大田种植期间从未发现病害。目前已在省内外适宜地区扩大生产应用。

关键词：细胞工程晚粳粳稻选育早熟优质

Effects of Estrogen Level on the Function of Vascular Endothelial Cells and Expression of Vascular Cell Adhesion Molecule-1(?)

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South China Journal of Cardiology 2002年第2期3卷 109-114页

作者：吴赛珠刘建国谭家余周可祥 Gorge D Webb 隗和明郭志刚 Department of Molecular & Biophysics University of Vermont College of Medicine U. S. A Department of Cardiology Nanfang Hospital Guangzhou 510515 P R China Atherosclerosis and Vascular Biology (AVB) Lab Johns Hopkins Singapore Pte Ltd

Objectives To ob-serve the effect of different estrogen levels on the secretory function of vascular endothelial cells of female rats, and study the effect of modulation of estrogen level on the expression of vascular cell adhesion molecule - 1 and the concentration of estrogen receptor in vascular endothelial cells. Methods Radioim-munology was used to measure the serum concentration of endothelin and PGI2, and copper - cadmium reduction was employed to measure the serum content of nitrogen monoxide. Radioligand binding and flowcy-tometry were used to measure the expression of estrogen receptor and vascular cell adhesion molecule (VCAM - 1) of vascular endothelial cells respectively. Results 1. The serum concentration of nitric oxide and PGI2 decreased when the ovaries of female rats were removed. In ovariectomized rats, given estrogen, the concentration rose ( P < 0. 05), but the plasma concentration of endothelin was adverse to it. 2. The concentration of estrogen receptor of vascular endothelial cells decreased remarkably when the ovaries of female rats were removed. When given estrogen, it increased. 3. The percent of expressed VCAM - 1 increased significantly after interleukin - 1βoperated on the cells, but 17 -βestradiol at 3 × 10-8 - 10-6 mol/l all decreased the percent. Conclusions Estrogen level can influence the secretion of nitrogen monoxide, PGI2 and endothlin of vascular endothelial cells, and also influence the concentration of estrogen receptor of vascular endothelial cells. 17 -β Estradiol at 3 × 10-8 -10-6 M can decrease the elevation of VCAM - 1 of vascular endothelial cells induced by interleukin - 1β.

关键词： Estrogen Vascular endothelial cell Estrogen receptor Vascular endothelial ad-hesion molecule

The Effect of Dehydroepiandrosterone on the Expression of AT_1 receptor and TNF-induced ICAM-1 in Vascular Smooth Muscle Cells

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South China Journal of Cardiology 2005年第1期6卷 22-27页

作者：吴赛珠王子东周忠江周可祥吴炎贤孙飞容志毅马瑞 WeiHeming DepartmentofCardiology NanfangHospitalSouthernMedicalUniversityGuangzhou510515China Atherosclerosis＆VascularBiologyLab JohnsHopkinsSingapore

Objectives To further invest- igate the molecular mechanism of vasoprotective role of dehydroepiandrosterone (DHEA), we examined DHEA on AT1 receptor and ICAM-1 gene expression in vascular smooth muscle cells (VSMCs). Methods RT-PCR and Western Blot was used to determine the change of the expressions of mRNA and protein of AT1 and ICAM- 1 when given various concentration dehydroepian- drosterone. Results ***1 was abundant under the basal condition. The expression of AT1 mRNA and protein decreased after stimulated by DHEA (at 10- 10mol/L , 10-8 mol/L, 10-6 mol/L) , and the effects of DHEA on AT1 protein was dose-dependent. ER inhibitor Tamoxifen and AR inhibitor Flutamide enhanced AT1 protein expression, but did not influence the mRNA expression. 2. The exp-ression of ICAM-1 gene was low under the basal *** increased when induced by TNF-α,but decreased when induced by DHEA (at 10-10 mol/L, 10-8 mol/L, 10-6 mol/L) , and the effects of DHEA on ICAM-1 gene expression were dose-dependent. Conclusions These findings suggest that DHEA modulates AT1 and inflammatory factor induced ICAM-1 gene expression in VSMC, but further studies are necessary in the mecha-nism of DHEA action.

关键词： Dehydroepiandrosterone Receptor AT_1 ICAM-1 Smooth muscle cells Atherosclerosis