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Use of nanoscale metals in poultry diet as a mineral feed additive

Animal Nutrition 2020年第2期6卷 185-191页

作者： еlena sizova Sergey Miroshnikov Svyatoslav Lebedev Boris Usha Sergey Shabunin Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences Orenburg460000Russia Orenburg State University Orenburg460018Russia Moscow State University of Food Production Moscow125080Russia Federal State Budget Scientific Institute(All-Russian Veterinary Research Institute of Pathology Pharmacology and Therapy)Voronezh394087Russia

The research was aimed at studying the efficiency of a nanoscale alloy of copper(Cu) and zinc(Zn) to be used as a mineral additive for feeding broiler chickens,compared to inorganic and organic forms of these *** studies of the blood serum were performed using an automated *** mineral composition was determined by atomic emission and mass spectrometry(MS-ISP),The study was performed on broiler chickens of cross Smena 7(n=72) in the conditions of a *** were3 treatment groups with 24 chickens in *** the inorganic form of mineral supplements with the nanosized alloy resulted in a positive productive effect,with a tendency to increasing the content of serum *** nano scale form of metals improved(P <0.05) the activity of *** the same time,the liver micro structure of experimental groups is similar to that of the *** was a moderate plethora and poor polymorphoncellular infiltration around the interlobular triads with a clear morphological orga nization of the stromal and parenchymal co mponents of the ***,the lack of oxidative stress was confirmed by the dynamics of catalase(CT),total superoxide dismutase(TSOD) and malondialdehyde(MDA) levels,and the concentrations of which did not exceed the reference level Replacing Cu and Zn sulfates with the nanoscale alloy(group 1) and organic form(group 2) of these elements in the diet of broiler chickens was accompanied by the increasing pool of these elements in the organisms at the end of the *** was accumulated throughout the experiment in experimental group 1,compared to the reference,with the maximum difference in the liver of 36.5%(P ≤ 0.05),in the feathers 2.5 times(P ≤ 0.01).Assessment of the Zn level dynamics in the feathers revealed a well noticeable tendency to reducing its concentrations during the experiment in all *** the background of feeding a nanoscale alloy,Zn concentration in the liver exceeded th

关键词： Broiler chicken Feeding Mineral supplement Nanoscale metal

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Newly-discovered ultra-high temperature granulites from the East Hebei terrane,North China Craton

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Science Bulletin 2022年第7期67卷 670-673页

作者： Ting Liu Chunjing Wei Tim E.Johnson Elena sizova College of Earth Sciences Chengdu University of TechnologyChengdu 610059China School of Earth and Space Sciences Peking UniversityBeijing 100871China School of Earth and Planetary Sciences the Institute for Geoscience ResearchCurtin UniversityPerthWA 6845Australia Institute of Earth Sciences University of GrazGraz 8010Austria

Archean crustal terranes are commonly featured with the dome–and–keel structures,in which supracrustal rocks occur as belts(greenstones)between,and rafts within,dome-like bodies of tonalite–trondhjemite–granodiorite(TTG).Supracrustal rafts within TTG gneisses generally record metamorphic grades ranging from amphibolite-to ultra-high temperature(UHT)granulitefacies of low-to moderate-pressure conditions[1].Notably,granulite-facies rafts commonly show counterclockwise P±T paths involving a significant increase in pressure at high temperatures[2,3].However,the tectonothermal drivers behind such metamorphism are still debated.

关键词： crustal 超高温麻粒岩 facies

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Copper Nanoparticles as Modulators of Apoptosis and Structural Changes in Tissues

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Journal of Biomaterials and Nanobiotechnology 2012年第1期3卷 97-104页

作者： Elena sizova Sergey Miroshnikov Valentina Polyakova Natalia Gluschenko Anatoly Skalny Orenburg State University Orenburg Russia

Results of research on copper nanoparticles influence on index of readiness to apoptosis and structural changes of liver, spleen, kidney tissues as well as sensomotor cerebral cortex under copper multiple introductions into organism of animals are presented in the article. It is established that copper nanoparticles distribute in organs and tissues of animals and cause specific structural changes. The increase of copper nanoparticles in organism up to toxical threshold (maximum tolerated dose) results in dystrophy and tissue necrosis. Accurate expression enhancement of Caspase 3 in micro-gliocytes (brain macrophages) has been registered seven days after one-fold intramuscular introduction of copper nanoparticles (dose 2 mg/kg of animal body weight), in liver cells - three and seven days after three-fold intramuscular introduction of copper nanoparticles (total dose was 6 mg/kg of animal body weight), in proximal kidney tubules-three hours, one, three and seven days after three-fold intramuscular introduction of copper nanoparticles (with total dose 6 mg/kg of animal body weight), in spleen cells three hours, one, three and seven days after 12-fold intramuscular introduction (with total dose 24 mg/kg of animal body weight). Received data enables us to propose using index of cells readiness to apoptosis defined by Caspase 3 expression as a criterion for copper nanoparticles introduction safety assessment.

关键词： Apoptosis Copper Nanoparticles Microelements Spleen Liver

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Ecological features of trace elements tolerant microbes isolated from sewage sludge of urban wastewater treatment plant

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Stress Biology 2024年第1期4卷 692-704页

作者： L.Perelomov V.D.Rajput M.Gertsen O.sizova I.Perelomova S.Kozmenko T.Minkina Y.Atroshchenko Tula State Lev Tolstoy Pedagogical University(Lev Tolstoy University) Lenin Avenue125Tula 300026Russia Academy of Biology and Biotechnology Southern Federal UniversityRostov-on-Don 344006Russia Federal Research Center“Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences” G.K.Skryabin Institute of Biochemistry and Physiology of Microorganisms of RASPushchino 142290Russia Tula State University Lenin Avenue92Tula 300026Russia

Worldwide wastewater treatment plants generate enormous amounts of sewage sludge,and their further disposal depends on the treatment technologies applied and spontaneously occurring microbiological *** different ages urban sewage sludge,12 strains of bacteria with simultaneous tolerance to two or more trace elements:Co,Ni,Cu,Zn,Cd and Pb at concentration of 3-5 mmol were isolated and identified by PCR of target genes and Sanger sequencing *** isloated metal(loids)tolerant strains belong to the species,i.e.,Serratia fonticola,Rhodococcus qingshengii,Pseudomonas fragi,Pseudomonas extremaustralis,Pseudomonas cedrina,Stenotrophomonas maltophilia,Serratia liquefaciens and Citrobacter *** ecological features of the isolated strains were *** optimal growth temperatures for most strains was 15-30°C at pH range of 5-9,although some strains grew at 7°C(Pseudomonas fragi SS0-4,Serratia fonticola SS0-9 and Serratia fonticola SS12-11).Satisfactory growth of two strains(Serratia fonticola SS0-1and Citrobacter freundii SS60-12)was noted in an acidic medium at pH *** of the strains grew in the NaCl concentration range of 1-5%.The isolated bacteria resistant to high concentrations of trace elements can be used for the effective mineralization of sewage sludge and for the decontamination of wastewater.

关键词： Microorganisms Heavy metals Metalloids Wastewater treatment plant Sewage sludge

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