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Remodeling of the cell wall as a drought-tolerance mechanism of a soybean genotype revealed by global gene expression analysis

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aBIOTECH 2021年第1期2卷 14-31页

作者： Flaviane silva Coutinho Juliano Mendonça Rodrigues Lucas Leal Lima Rosilene oliveira Mesquita Paola Avelar Carpinetti João Paulo Batista Machado Camilo Elber Vital Pedro Marcus Vidigal Maria Eduarda Soares Ramos Mariana Rocha Maximiano Angela Mehta Maria Goreti Almeida oliveira Elizabeth Pacheco Batista Fontes Humberto Josuéde oliveira Ramos departamento de Bioquímica e Biologia Molecular Universidade Federal de ViçsaViçsaMinas GeraisBrazil departamento de Fitotecnia Universidade Federal do CearáFortalezaCearáBrazil Núcleo de Análise de Biomoléculas(NuBioMol) Universidade Federal de ViçsaViçsaMinas GeraisBrazil Instituto de Ciéncias Agrárias Universidade Federal de ViçsaFlorestalMinas GeraisBrazil Embrapa Recursos Genéticos e Biotecnologia BrasíliaDFBrazil Universidade Federal de Juiz de Fora Juiz de ForaMinas GeraisBrazil

Drought stress is major abiotic stress that affects soybean ***,it is widely desirable that soybean becomes more tolerant to *** provide insights into regulatory mechanisms of the stress response,we compared the global gene expression profiles from leaves of two soybean genotypes that display different responses to water-deficit(BR 16 and Embrapa 48,drought-sensitive and droughttolerant,respectively).After the RNA-seq analysis,a total of 5335 down-regulated and 3170 up-regulated genes were identified in the *** the other hand,the number of genes differentially expressed was markedly lower in the Embrapa 48,355 up-regulated and 471 down-regulated ***,induction and expression of protein kinases and transcription factors indicated signaling cascades involved in the drought ***,the results suggest that the metabolism of pectin is differently modulated in response to drought stress and may play a role in the soybean defense mechanism against *** occurs via an increase of the cell wall plasticity and crosslink,which contributed to a higher hydraulic conductance(Kf)and relative water content(RWC%).The drought-tolerance mechanism of the Embrapa 48 genotype involves remodeling of the cell wall and increase of the hydraulic conductance to the maintenance of cell turgor and metabolic processes,resulting in the highest leaf RWC,photosynthetic rate(A),transpiration(E)and carboxylation(A/Ci).Thus,we concluded that the cell wall adjustment under drought is important for a more efficient water use which promoted a more active photosynthetic metabolism,maintaining higher plant growth under drought stress.

关键词： Gene expression RNA-seq Molecular physiology Water-use efficiency

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C57BL/6 and DBA/1 Mice Differ in Their Response to Supplementation with 1,25D and Paricalcitol

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Biomedical and Environmental Sciences 2018年第8期31卷 613-618页

作者： Aline Parisoto Missio Thais Fernanda de Campos Fraga-silva Larissa Lumi Watanabe Ishikawa Luiza Ayumi Nishiyama Mimura Thais Graziela Donega Franga Larissa Ragozo Cardoso de oliveira Alexandrina Sartori Sofia Femanda Goncalves Zorzella-Pezavento department of Microbiology and Immunology Sao Paulo State University(UNESP)Institute of BiosciencesBotucatuSao PauloBrazil

Active vitamin D (1,25D) is a fat-soluble vitamin that is mainly produced in the skin by the conversion of 7-dehydrocholesterol under ultraviolet light *** role in calcium homeostasis,bone growth, and prevention of ri... 详细信息

关键词： DBA BL C57BL/6 and DBA/1 Mice Differ in Their Response to Supplementation with 1,25D and Paricalcitol

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Associations between sociodemographic factors and physical activity and sedentary behaviors in adults with chronic diseases during COVID-19 pandemic

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Sports Medicine and Health Science 2020年第4期2卷 216-220页

作者： Jean Marinho da silva Rozangela Verlengia JoséJonas de oliveira Anna Gabriela silva Vilela Ribeiro Carolina Gabriela Reis Barbosa Uliana Sbeguen Stotzer Alex Harley Crisp Post-Graduate Program in Human Movement Sciences Methodist University of Piracicaba(UNIMEP)PiracicabaSPBrazil Physical Education department Faculty of S~ao Lourenço(UNISEPE)S~ao LourençoMinas GeraisBrazil Physical Education department University Centre of Itajubá(FEPI)ItajubáMinas GeraisBrazil

The pandemic of the new coronavirus(COVID-19)may be affecting the physical activity(PA)level in much of the *** study aimed to investigate the prevalence of physical inactivity and sedentary behavior(SB)among adults with chronic diseases and their associations with sociodemographic factors during the COVID-19 *** cross-sectional study included 249 participants(age:18-91 years;61.4%female)with chronic conditions and attended the Family Health Strategy program in a small town in *** were collected between 2020-07-13 and 2020-07-24 by face-to-face ***-reported PA,sitting time,chronic diseases,medication use,sociodemographic data,and self-isolation adherence were obtained by *** this specific time point of the COVID-19 pandemic,71.5%of participants did not meet the PA recommendations(≥500 METs-min/week),and the prevalence of SB risk(≥4 h sitting)was 62.7%.Adjusted logistic regression indicated that male participants(odds ratio[OR]:1.89[95%CI 1.02-3.53]),living alone(OR:2.92[95%CI 1.03-8.30])or in a two-person household(OR:2.32[95%CI 1.16-4.63]),and those who reported sometimes performing self-isolation(OR:3.07[95%CI 1.47-6.40])were more likely to meet the minimum PA *** smokers had a lower odds(OR:0.36[95%CI 0.14-0.95])of meeting the PA *** participants(OR:2.18[95%CI 1.06-4.50])and those who had multimorbidity(OR:1.92[95%CI 1.07-3.44])were more likely to have a higher degree of *** is an urgent need to mitigate physical inactivity and SB,and public health interventions must take into account sociodemographic status.

关键词： Moderate-to-vigorous physical activity Sitting time Morbidity Coronavirus Quarantine

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Epigenetic regulation of TP53 is involved in prostate cancer radioresistance and DNA damage response signaling

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Signal Transduction and Targeted Therapy 2023年第11期8卷 5404-5416页

作者： Catarina Macedo-silva Vera Miranda-Gonçalves Nuno Tiago Tavares Daniela Barros-silva Joana Lencart João Lobo Ângelo oliveira Margareta PCorreia Lucia Altucci Carmen Jerónimo Cancer Biology&Epigenetics Group Research Center of IPO Porto(CI-IPOP)/CI-IPOP@RISE(Health Research Network)Portuguese Oncology Institute of Porto(IPO-Porto)/Porto Comprehensive Cancer Center Raquel Seruca(Porto.CCC)R.Dr.António Bernardino de Almeida4200-072PortoPortugal department of Precision Medicine University of Campania“Luigi Vanvitelli”80138NaplesItaly department of Pathology and Molecular Immunology ICBAS-School of Medicine&Biomedical SciencesUniversity of PortoR.Jorge de Viterbo Ferreira 2284050-313PortoPortugal Medical Physics Radiobiology and Radiation Protection Group-Research Center of IPO Porto(CI-IPOP)/CI-IPOP@RISE(Health Research Network)Portuguese Oncology Institute of Porto(IPO-Porto)/Porto Comprehensive Cancer Center Raquel Seruca(Porto.CCC)R.Dr.António Bernardino de Almeida4200-072PortoPortugal department of Medical Physics Portuguese Oncology Institute of Porto4200-072PortoPortugal department of Pathology Portuguese Oncology Institute of PortoPortoPortugal department of Radiation Oncology Portuguese Oncology Institute of PortoPortoPortugal BIOGEM Molecular Biology and Genetics Research Institute83100AvellinoItaly IEOS Institute of Endocrinology and Oncology80100NaplesItaly

External beam radiotherapy(RT)is a leading first-line therapy for prostate cancer(PCa),and,in recent years,significant advances have been ***,RT resistance can arise and result in long-term recurrence or disease progression in the worst-case ***,making crucial the discovery of new targets for PCa ***,we generated a radioresistant PCa cell line,and found p53 to be highly expressed in radioresistant PCa cells,as well as in PCa patients with recurrent/disease progression submitted to *** dissection revealed that RT could promote p53 expression via epigenetic ***,a decrease of H3K27me3 occupancy at TP53 gene promoter,due to increased KDM6B activity,was observed in radioresistant PCa ***,p53 is essential for efficient DNA damage signaling response and cell recovery upon stress induction by prolonged fractionated ***,KDM6B inhibition by GSK-J4 significantly decreased p53 expression,consequently attenuating the radioresistant phenotype of PCa cells and hampering in vivo 3D tumor ***,this work contributes to improve the understanding of p53 as a mediator of signaling transduction in DNA damage repair,as well as the impact of epigenetic targeting for PCa radiosensitization.

关键词： TP53 damage cancer

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Therapeutic and biological activities of Calotropis procera(Ait.) ***.

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Asian Pacific Journal of Tropical Medicine 2010年第4期3卷 332-336页

作者： Marcia Calheiros Chaves silva Antonio Batista da silva Fabiano Moura Teixeira Paulo Cesar Pereira de Sousa Regina Maria Macedo Rondon Jose Eduardo Ribeiro Honorio Junior Luiz Rafael Leite Sampaio Samanda Lima oliveira Angela Nadyla Martins Holonda silvania Maria Mendes de Vasconcelos Enfermeira Mestre em Farmacologia/Universidade Federal do Ceara/UFC Farmaceutico Mestre em Patologia Tropical/Universidade Federal do Amazonas/UFAM Biologo Mestre em Bioquimica/Universidade Federal do Ceara/UFC Farmaceutico Mestre em Ciencias Farmaceutica/Universidade Federal do Ceara/UFC Biologa Mestre em Producao Animal/Universidade Estadual do Ceara/UECE Biologo Mestre em Fisiologia da Universidade Estadual do Ceara/UECE Enfermeiro Mestrando em Farmacologia da Universidade Federal do Ceara/UFC Aluna de Farmacia da Universidade Federal do Ceara/UFC Enfermeira Professora Adjunto do Depto.de Fisiologia e Farmacologia da Universidade Federal do Ceara/UFC

Medicinal plants have been used to treat various ailments of the poor population around the world;hence the interest among researchers to know the active ingredients of certain plants has being *** Calotropis procera(***) is a plant original from Africa,commonly found in northeastern *** is well known for their pharmacological properties,since it produces large amounts of *** important role that medicinal plants play in folk medicine has led us to develop this article in order to review the major pharmacological activities of C. procera.

关键词： Calotropis procera Pharmacological activity Medicinal plants

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Object-based image analysis supported by data mining to discriminate large areas of soybean

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International Journal of Digital Earth 2019年第3期12卷 270-292页

作者： Carlos Antonio da silva Junior Marcos Rafael Nanni José Francisco de oliveira-Júnior Everson Cezar Paulo Eduardo Teodoro Rafael Coll delgado Luciano Shozo Shiratsuchi Muhammad Shakir Marcelo Luiz Chicati Geotechnology Applied in Agriculture and Forest(GAAF) State University of Mato Grosso(UNEMAT)Alta FlorestaMato GrossoBrazil department of Agronomy(DAG/PGA) State University of Maringá(UEM)MaringáParanáBrazil Institute of Atmospheric Sciences(ICAT) Federal University of Alagoas(UFAL)MaceióAlagoasBrazil Federal University of Mato Grosso do Sul(UFMS) Chapadão do SulMato Grosso do SulBrazil department of Environmental Sciences(DCA) Forestry Institute(IF)Federal Rural University of Rio de Janeiro(UFRRJ)SeropédicaRio de JaneiroBrazil AgCenter Louisiana State University(LSU)Baton RougeLAUSA Institute of Space Technology Islamabad Pakistan IslamabadPakistan

This research aimed to analyze the possibility to estimate and automatically map large areas of soybean cultivation through the use of MODIS(Moderate-Resolution Imaging Spectroradiometer)*** major techniques were used:GEOgraphic-Object-Based Image Analysis(GEOBIA)and Data Mining(DM).In order to obtain the images,the segmentation algorithm implemented by definiens developer was used.A decision tree(DT)was created from a training set previously ***-series of images from the MODIS sensor aboard the Terra satellite were acquired in order to represent the wide variation of the vegetation pattern along the soybean crop *** time-series data were used only for the CEI ***,to compare the results obtained from GEOBIA,the slicing technique was used at the CEI *** the training,the DT was applied to the vegetation indices generating the thematic map of the spatial distribution of *** accordance with the error matrix and kappa parameter analysis,tests for statistical significance were *** indicate that the classification achieved by Kappa coefficients is *** short,the obtained results proved that combining vegetation indices and time-series data using GEOBIA return promising results for mapping soybean plantation on a regional scale.

关键词： GEOBIA time-series vegetation indices MODIS vegetation dynamics

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Evaluation of the regional distribution of the pediatric surgery workforce and surgical load in Brazil

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World Journal of Pediatric Surgery 2023年第2期6卷 85-91页

作者： Joaquim Bustorff-silva Marcio Lopes Miranda Amanda Rosendo Ayla Gerk Antonio Goncalves oliveira-filho Pediatric Surgery State University of Campinas Medical SchoolCampinasBrazilPediatric SurgeryState University of Campinas Medical SchoolCampinasBrazil Medicine Pontificia Universidad Católica ArgentinaBuenos AiresArgentina

Objective The purpose of this study is to examine the regional distribution of the pediatric surgery workforce and the expected local demand for pediatric surgical procedures in *** We collected data on the pediatric surgical workforce,surgical volume,Gross Domestic Product per capita,and mortality for gastrointestinal tract malformations(MGITM)across the different regions of Brazil for *** Data from the Federal Medical Council reported 1515 pediatric surgery registries in Brazil,corresponding to 1414 pediatric surgeons(some pediatric surgeons are registered in more than one state),or 2.4 pediatric surgeons per 100000 children 14 years of age and *** were 828 men and 586 *** mean age was 51.5±12.8 years,and the mean time from graduation was 3.4±5.7 *** is a higher concentration of pediatric surgeons in the wealthier Central-West,South,and Southeast *** surgical volume ranged from 88 to 245 operations/year(average 146 operations/year)depending on the *** these,only nine(6.1%)were high-complexity(including neonatal)*** tended to be higher in the poorer North and Northeast regions than in other regions of *** Our findings suggest significant disparities in the surgical workforce and workload across Brazil related to socioeconomic *** with an increased surgical workforce were associated with lower *** average number of complex operations performed annually by each pediatric surgeon was considerably *** investment and well-defined health policies are imperative to enhance the quality of surgical care in the different regions of Brazil.

关键词： pediatric operations distribution

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Impact of SARS-CoV-2 infection during pregnancy on postnatal brain development:The potential role of glial cells

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BIOCELL 2022年第12期46卷 2517-2523页

作者： LARISSA DANIELE BOBERMIN LARA SCOPEL MEdeIROS FERNANDA WEBER GIANCARLO TOMAZZONI de oliveira LUCÉLIA SANTI WALTER ORLANDO BEYS-DA-silva CARLOS-ALBERTO GONÇALVES ANDRÉQUINCOZES-SANTOS Programa de Pós-Graduação emNeurociências Instituto de Ciências Básicas da SaúdeUniversidade Federal do Rio Grande do SulPorto Alegre90050170Brazil departamento de Bioquímica Instituto de Ciências Básicas da SaúdeUniversidade Federal do Rio Grande do SulPorto Alegre90035003Brazil Faculdade de Farmácia Universidade Federal do Rio Grande do SulPorto Alegre90610000Brazil Hospital de Clínicas de Porto Alegre Porto Alegre90035903Brazil Programa de Pós-Graduação emBiologia Celular eMolecular Centro de BiotecnologiaUniversidade Federal do Rio Grande do SulPorto Alegre91501970Brazil Programa de Pós-Graduação em Ciências Biológicas:Bioquímica Instituto de Ciências Básicas da SaúdeUniversidade Federal do Rio Grande do SulPorto Alegre90035003Brazil

Glial cells are crucial for maintaining central nervous system(CNS)*** actively participate in immune responses,as well as form functional barriers,such as blood-brain barrier(BBB),which restrict the entry of pathogens and inflammatory mediators into the *** general,viral infections during the gestational period can alter the embryonic and fetal environment,and the related inflammatory response may affect neurodevelopment and lead to behavioral dysfunction during later stage of life,as highlighted by our group for Zika virus *** acute respiratory syndrome coronavirus-2(SARS-CoV-2)induces a cytokine storm and,during pregnancy,may be related to a more severe form of the coronavirus disease-19(COVID-19)and also to higher preterm birth ***-CoV-2 can also affect the CNS by inducing neurochemical remodeling in neural cells,which can compromise neuronal plasticity and synaptic ***,the impact of SARS-CoV-2 infection during pregnancy on postnatal CNS,including brain development during childhood and adulthood,remains *** group has recently highlighted the impact of COVID-19 on the expression of molecular markers associated with neuropsychiatric disorders,which are strongly related to the inflammatory ***,based on these relationships,we discussed the impact of SARS-CoV-2 infection either during pregnancy or in critical periods of neurodevelopment as a risk factor for neurological consequences in the offspring later in life,focusing on the potential role of glial ***,it is important to consider future and long-term public health concerns associated with SARS-CoV-2 infection during pregnancy.

关键词： Brain development Glial cells Inflammation Pregnancy SARS-CoV-2

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Y-chromosome microdeletions in nonobstructive azoospermia and severe oligozoospermia

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Asian Journal of Andrology 2017年第3期19卷 338-345页

作者： Carolina Goncalves Mariana Cunha Eduardo Rocha Susana Fernandess, Joaquina silva Luis Ferraz Cristiano oliveira Alberto Barros Mairio Sousa department of Biology ClCECO University of Aveiro Campus Universitario de Santiago 3810-193 Aveiro Portugal department of Microscopy Laboratory of Cell Biology Institute of Biomedical Sciences Abel Salazar University of Porto (ICBAS-UP) Rua Jorge Viterbo Ferreira 228 4050-313 Porto Portugal and Multidisciplinary Unit for Biomedical Research ICBAS-UP Portugal Centre for Reproductive Genetics Prof. Alberto Barros Av. do Bessa 240 1° Dto. Frente 4100-009 Porto Portugal department of Microscopy Laboratory of Histology and Embryology ICBAS-UP Rua Jorge Viterbo Ferreira 228 4050-313 Porto Portugal department of Genetics Faculty of Medicine University of Porto Alameda Prof. Hernani Monteiro 4200-319 Porto Portugal and Institute for Innovation and Health Research (13S) University of Porto Portugal department of Urology Hospital Center of Vila Nova de Gala Rua Conceicao Fernandes 4430-502 Vila Nova de Gala Portugal.

The aim of the present work was to present the outcomes of the patients with Y-chromosome microdeletions treated by intracytoplasmic sperm injection （ICSI）, either using fresh （TESE） or frozen-thawed （TESE-C） testicular sperm and ejaculated sperm （EJAC）. The originality of this work resides in the comparisons between the different types of Y-microdeletions （AZFa, AZFb, and AZFc） and treatments, with detailed demographic, stimulation, embryological, clinical, and newborn （NB） outcomes. Of 125 patients with Y-microdeletions, 33 patients presented severe oligozoospermia （18 performed ICSI with ejaculated sperm） and 92 secretory azoospermia （65 went for TESE with 40 having successful sperm retrieval and performed ICSI）. There were 51 TESE treatment cycles and 43 TESE-C treatment cycles, with a birth of 19 NB （2 in AZFa/TESE-C, 12 in AZFc/TESE, and 5 in AZFc/TESE-C）. Of the 29 EJAC cycles, there was a birth of 8 NB （in AZFc）. In TESE and EJAC cycles, there were no significant differences in embryological and clinical parameters. In TESE-C cycles, there was a significant lower oocyte maturity rate, embryo cleavage rate and mean number of embryos transferred in AZFb, and a higher mean number of oocytes and lower fertilization rate in AZFc. In conclusion, although patients with AZFc microdeletions presented a high testicular sperm recovery rate and acceptable clinical outcomes, cases with AZFa and AZFb microdeletions presented a poor prognosis. Due to the reported heredity of microdeletions, patients should be informed about the infertile consequences on NB and the possibility of using preimplantation genetic diagnosis for female sex selection.

关键词： intracytoplasmic sperm injection newborn outcomes nonobstructive azoospermia severe oligozoospermia testicularsperm extraction Y-chromosome microdeletions

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Diversity of the Formicidae (Hymenoptera) carrion communities in Lisbon (Portugal): preliminary approach as seasonal and geographic indicators

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Forensic Sciences Research 2018年第1期3卷 65-73页

作者： António Neto-silva ricardo Jorge Dinis-oliveira Catarina Prado e Castro department of Public Health Forensic Sciences and Medical EducationFaculty of MedicineUniversity of PortoPortoPortugal department of Sciences IINFACTS-Institute of Research and Advanced Training in Health Sciences and TechnologiesUniversity Institute of Health Sciences(IUCS)CESPUCRLGandraPortugal UCIBIO REQUIMTE-Laboratory of ToxicologyDepartment of Biological SciencesFaculty of PharmacyUniversity of PortoPortoPortugal department of Life Sciences Centre for Functional EcologyUniversity of CoimbraCoimbraPortugal

The value of the Formicidae (Insecta,Hymenoptera) community in forensic investigations is poorly studied in *** order to better understand the structure and dynamics of this group of insects in cadavers and their putative value in forensic investigations,studies were carried out in Lisbon area during one *** carcasses were used as a model of human *** entomofauna attracted to the carcasses was collected over a period of about 2 months in each season of the *** collection of ants was performed at regular intervals,daily in the first 23 days and then with intervals of 2,3 or 5 days until the end of the *** stages of cadaveric decomposition were recognized with the ants being present in all of *** hundred and nine specimens were captured: 7 in autumn,6 in winter,90 in spring and 206 in *** specimens belong to three subfamilies and seven different *** of them had never been mentioned before as being associated to cadavers in this geographical *** and summer had the greater diversity of species and a larger number of *** luteus was the dominant species in spring and Tapinoma nigerrimum in *** the information of other similar studies carried out in the Iberian Peninsula,the sarcosaprophagous Formicidae community found in Lisbon is unique and different from other studied locations,which supports the need to perform regional *** results shown that ants do not present a definite pattern of succession,but some species have the potential to be seasonal or geographic indicators.

关键词： Forensic science forensic entomology Formicidae sarcosaprophagous community decomposition process postmortem interval seasonal study

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