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Determination of gemcitabine and its metabolite in human plasma using high-pressure liquid chromatography coupled with a diode array detector

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Acta Pharmacologica Sinica 2004年第12期25卷 18-23页

作者： Neng-ming LIN2,3. Su ZENG2,4, Sheng-lin MA3. Yun FAN3. Hai-jun ZHONG3. Luo FANG3. 2Collegeof Pharmaceutical Sciences, zhejiang university, hangzhou 3.003. 3.2} Cancer Hospital, hangzhou 3.0022, china College of Pharmaceutical Sciences Zhejiang University Hangzhou 310031 China

AIM: To establish a high pressure liquid chromatography (HPLC) method for determination of the concentration of gemcitabine (dFdC) and its metabolite(dFdU) in human plasma. METHODS: Plasma 1.0 mL spikedwith floxuridine as an internal standard was extracted with 3.0 mL of methanol-acetonitrile (v/v, 1:9). The supernatant was evapo- rated at 60 oC and the residue was reconstituted with 0.5 mL of the solution used as the mobile phase. After centrifugation, 50 μL of the supernatant was injected into the HPLC system. Separation was achieved on a C18 (4.6 mm×250 mm, 5 μm) column at 25 oC with the flow rate of the mobile phase set to 0.8 mL/min. The compounds were detected at 268 nm. The mobile phase consisted of 40.0 mmol/L acetate ammonium buffer solution (pH 5.5) and acetonitrile (v/v, 97.5:2.5). RESULTS: The linear range was 0.20-10.0 mg/L(r=0.9999) for dFdC and 0.50-50.0 mg/L(r=0.9999) for dFdU. The limit of detection (LOD) was 0.10 mg/L for dFdC and 0.25 mg/L for dFdU, while the limit of quantification (LOQ) was 0.20 mg/L (RSD3.%) for dFdU. The average recovery of dFdC and dFdU by this method were 103.3.% and 98.7 %, respectively. For intra-day and inter-day, the corresponding standard deviations of the measurements of dFdC and dFdU were both less than 5.5 %. CONCLUSION: An analytical method was established to measure the concentrations of dFdC and dFdU in human plasma and was effectively applied to the dFdC and dFdU pharmacokinetic studies of 8 Chinese patients with malignant tumors.

关键词： high pressure liquid chromatography gemcitabine deoxycytidine floxuridine

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A Microchip Tunable Blue Laser Source Based upon Cr∶LiSAF in Contact with KN Crystal

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Chinese Journal of Lasers 2002年第1期11卷 6-9页

作者： MENG Hongxiang (Dept. of Optical Engineering, zhejiang university, hangzhou 3.0027, china) HE Liangfang (Dept. of Applied Physics, zhejiang university of Technology, hangzhou 3.0014, china) Dept. of Optical Engineering Zhejiang University Hangzhou 310027 Dept. of Applied Physics Zhejiang University ofTechnology Hangzhou 310014

In this paper, a microchip laser consisted of 2.2% doped Cr∶LiSAF crystal and NCPM KN(KNbO 3. crystal and pumped by a 679 nm laser diode is reported. The maximal output of TEM 00 4.76 mW of blue laser at 43..7 nm is obtained with the absorption power of 3.4.6 mW. The laser shows a threshold of 76.2 mW and slope efficiency of 2.08%. Continuous-wave tunable output is demonstrated in the blue region of 423.4～445.5 nm through only changing temperature of KN crystal controlled by PID controller.

关键词： LD pumping intracavity double Cr∶LiSAF tunable blue lasers

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The three-dimensional structure of Infectious flacherie virus capsid determined by cryo-electron microscopy

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Science china(Life Sciences) 2009年第12期52卷 1186-1191页

作者： XIE Li1,4, ZHANG QinFen2·, LU XingMeng3. DAI XinHong2, LI KunPeng2, HONG Jian1,4· & ZHOU XuePing11 institute of Biotechnology, zhejiang university, hangzhou 3.0029, {3. 2 State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen university, Guangzhou 510275, {3. 3.College of Animal Sciences, zhejiang university, hangzhou 3.0029, {3. 4 Center of Analysis and Measurement, zhejiang university, hangzhou 3.0029, {3. institute of Biotechnology Zhejiang University Hangzhou 310029 China State Key Laboratory of Biocontrol School of Life Sciences Sun Yat-Sen University Guangzhou 510275 China College of Animal Sciences Zhejiang University Hangzhou 310029 China Center of Analysis and Measurement Zhejiang University Hangzhou 310029 China

Cryo-electron microscopy and image reconstruction were used to determine the three-dimensional structure of Infectious flacherie virus (IFV). 5047 particles were selected for the final reconstruction. The FSC curve showed that the resolution of this capsid structure was 18 ·. The structure is a psuedo T=3.(P=3. icosahedral capsid with a diameter of 3.2.4 · and a single shell thickness of 15 ·. The density map showed that IFV has a smooth surface without any prominent protrude or depression. Comparison of the IFV structure with those of the insect picorna-like virus-Cricket paralysis virus (CrPV)and human picornavirus-Human rhinovirus 14 (HRV 14) revealed that the IFV structure resembles the CrPV structure. The "Rossmann canyon" is absent in both IFV and CrPV particles. The polypeptide topology of IFV VP2, IFV VP3.was predicted and the subunit location at the capsid surface was further analyzed.

关键词： Infectious flacherie virus cryo-electron microscopy three-dimensional structure polypeptide folding

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Photodegradation of α-naphthaleneacetic acid in aqueous solution

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Journal of Environmental Sciences 2001年第3期13卷 361-364页

作者： LIU Hui jun, ZHOU Zhu fei, LIU Wei ping (Environmental Science institute, zhejiang university, hangzhou 3.0027, {3.. Environmental Science institute Zhejiang University Hangzhou 310027 China.

Kinetic processes of a-naphthaleneacetic acid (NAA) photolysis were studied under different conditions. The results showed that the ultraviolet light was more effective than fluorescent light in promoting degradation, and the degradation of NAA under ultraviolet light followed the first order kinetics with the photolysis rate constant of 1.15 x 10-2 min-1 and half-life time (t1/2) of 60 min. Further, it was proved that the photolysis rate was higher in the presence of oxygen, titanium dioxide (TiO2), and low pH (acidic solution). At last, two photolysis intermediates were identified by GC-MS and possible photolysis pathways were proposed.

关键词： Acids Degradation Fluorescence Photolysis Solutions

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Nonlinear properties of fractal growth of thin films at low temperature

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Communications in Nonlinear Science and Numerical Simulation 2001年第2期6卷 61-67页

作者： Fengmin WU, Jiefang ZHANG, Gen BIAN and Ziqin WU Research Center of Science, zhejiang university of Technology, hangzhou 3.0014, china {3. of Nonlinear Physics, zhejiang Normal university, Jinhua 3.1004, china Shao Yifu Hospital of zhejiang Provin Research Center of Science Zhejiang University of Technology Hangzhou 310014 China institute of Nonlinear Physics Zhejiang Normal University Jinhua 321004 China Shao Yifu Hospital of zhejiang Province Hangzhou 310005 China Department of Astronomy and Applied Physics University of Science and Technology of China Hefei 230026 China

Fractal growth of thin films at low temperature (75-200 K) is simulated by kinetic Monte Carlo method, using realistic growth model-Ag on Pt(111) surface and physical parameters. It is shown that the fractal growth of thin films is nonlinear, i. e., the island size S(t) grows with time t as tk ) where the growth exponent k is slightly less than 1 because of the growth competition of islands and the competition between nucleation and growth of islands. The further results indicate that the branch width of islands b increases with increasing island size S as b -Sa, where a is approximately equal to 1/3.because of the geometrical structure of the triangular substrate.

关键词： fractal growth thin film Monte Carlo method

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MECHANISM OF DEUTERIUM CRACKING IN PALLADIUM

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Acta Metallurgica Sinica(English Letters) 1993年第2期6卷 136-139页

作者： SUN Dalin LEI Yongquan CHEN Yanglin WU Jing WANG Qidong zhejiang university,hangzhou,china Department of Materials Science and Engineering,zhejiang university,hangzhou 3.0027,china zhejiang Univ Hangzhou China

After 290 h electrochemical charging of deuterium,the density of dislocation in well-annealed Pd was increased from 10~8/cm^2 to over 10~12/cm^2 and a large amount of bubble-like voids or cracks was observed by optical and transmission electron *** mechanism of deuterium cracking is believed due to the formation of molecular De during the segregstion and combination of deuterium atoms around *** results showed that the lattice constant of Pd increased from 0.3.90 nm to 0.403. nm.

关键词： deuterium cracking Pd

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Large-deviation probabilities for maxima of sums of subexponential random variables with application to finite-time ruin probabilities

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Science china Mathematics 2008年第7期51卷 1257-1265页

作者： JIANG Tao School of Finance,zhejiang Gongshang university,hangzhou 3.0018,{3. 1. School of Finance Zhejiang Gongshang University Hangzhou 310018 China

We establish an asymptotic relation for the large-deviation probabilities of the maxima of sums of subexponential random variables centered by multiples of order statistics of *** uniform random *** extends a corresponding result of *** an application,we generalize a result of Tang,the uniform asymptotic estimate for the finite-time ruin probability,to the whole strongly subexponential class.

关键词： large-deviation probability strongly subexponential distribution finite-time ruin probability the compound Poisson model uniform asymptotics 91B3. 60G70 62P05

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Transcriptional profile in response to ionizing radiation at low dose in Deinococcus radiodurans

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Progress in Natural Science:Materials International 2007年第5期17卷 529-536页

作者： Chen Huan, Xu Zhenjian , Tian Bing , Chen Weiwei , Hu Songnian and Hua Yuejin (institute of Nuclear-Agricultural Science, zhejiang university, hangzhou 3.0029, {3. James D. Watson institute of Genome Sciences, zhejiang university, hangzhou 3.0008, {3. Beijing institute of Genomics, Chinese Academy of Sciences, Beijing 1013.0, {3.) [a]institute of Nuclear-Agricultural Science Zhejiang University Hangzhou China [b]James D. Watson institute of Genome Sciences Zhejiang University Hangzhou China [c]Beijing institute of Genomics Chinese Academy of Sciences Beijing China

The genome-wide transcription profile of Deinococcus radiodurans cells was investigated after treatment with low dose irradiation (2 kGy). From the expression profile, we found that the process of DNA repair was induced in order, i.e. genes involved in base excision repair, nucleotide excision repair and single-strand annealing were induced immediately after ionizing radiation, and genes for recombination repair, including recA, recD and recQ were then activated. Especially, recD and recQ were specifically induced at low dose irradiation, and this phenomenon informed us that these two genes would play a certain role in anti-oxidation. Some genes such as ddrA and ssb were activated during the whole repair phase. Furthermore, the response of oxidative stress-related genes under low dose irradiation showed a different pattern from that of the acute high-level irradiation, many anti-oxidative genes were induced to scavenge reactive oxygen species directly, other associated systems also changed their expression patterns during the recovery time, such as iron metabolism systems, intracellular mutagenic precursors sanitize systems. These characteristics indicate that there is a powerful and orderly recovery process in Deinococcus radiodurans.

关键词： Deinococcus radiodurans microarry oxidative stress ionizing radiation.

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SOME THERMODYNAMIC PROPERTIES OF Pd-In ALLOYS

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Acta Metallurgica Sinica(English Letters) 1997年第4期10卷 311-317页

作者： YL. Chen(Department of Materials Science and Engineering, zhejiang university, hangzhou 3.0027, china) zhejiang Univ Hangzhou China

Some thermodynamic parnmeters of Pd-In alloys with 2 to 70 at. H indium have been determined in the temperuture range 800 to 1000℃ from e.m.f measurements ongalvanic cells with an onsen-cotzducting solid electrolyte. Heats of tnising calculated hem the activities are in excellent agreement with calorimetric data. The relative partial excess hee enengy of indiurn assumes unnsually larpe negative values ap to-174kJ/mol for Pd-rich alloys. Two efficts are discussed to account for the non-idealproperties: The rise Of Fermi enerpy as the valence electron8 of indium enter the 4dand 5sP bands of the alloy and the lattice distortion brought about by the differentmolar volumes of the cofnponents. The rise of the Fermi enerpy, as determlned fromthe activity data, indicates a strict adherence of the alloys to the rigid band model.

关键词： galvanic cell activity data Fermi energy,lattice distortion rigid band model

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ON CONVERGENCE OF NOUREIN ITERATIONS FOR SIMULTANEOUS FINDING ALL ZEROS OF A POLYNOMIAL

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Journal of Computational Mathematics 2000年第2期18卷 113-122页

作者： Shi-mingZheng Zheng-da Huang (Department of Mathematics, Xixi Campus, zhejiang university, hangzhou 3.0028, china) 浙江大学浙江杭州 310028

Presents the first estimation conditions for Nourein iterations for simultaneous finding all zeros of a polynomial under which the iteration processes are guaranteed to converge. Computational formulas; Theorems and p... 详细信息

关键词： polynomial zeros parallel iteration Nourein iterations point estimation convergence

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