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Exploration of the mechanisms of Ge Gen Decoction against influenza A virus infection

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Chinese Journal of Natural Medicines 2019年第9期17卷 650-662页

作者： GEng Zi-Kai LI Ya-qun cui Qing-Hua DU Rui-Kun TIAN Jing-Zhen School of Pharmacy Shandong University of Traditional Chinese MedicineJinan 250355China. Collaborative Innovation Center for Antiviral Traditional Chinese Medicine Jinan 250355China

Ge Gen Decoction(GGD),a Traditional Chinese Medicine prescription,is mainly used to treat infectious respiratory diseases and can relieve the symptoms of influenza A virus(IAV)***,the underlying mechanism of GGD against IAV infection remains *** this study,we found that GGD had moderate anti-IAV activity in *** was more effective when given before the viral infection and targeted the viral attachment and replication stages rather than the internalization sta *** vivo,GGD treatment reduced the virus titers of lung tissue significantly and improved the survival rate,lung index,and pulmonary hist opathological changes in H1N1-infected *** observed the changes in several key immuno-related indexes in GGD administrated H1N1-infected mice with anti-IAV drug oseltamivir phosphate as the *** treatment decreased the expression of TNF-αand improved Th1/Th2 immune balance to reduce the excessive immune response in H1N1-infected ***,the expression of the toll-like receptor 7 signaling pathway in H1N1-infected mice decreased after GGD *** results showed that GGD has anti-IAV activity and can modulate the immune system to relieve lung inflammation.

关键词： Ge Gen Decoction Traditional Chinese Medicine(TCM) H1N1 Antiviral Immune regulation

CIVB flashback analysis of hydrogen flame based on azimuthal vorticity at mixing zone exit

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Science China(Technological Sciences) 2014年第12期57卷 2466-2474页

作者： TIAN Xiao Jing XIng Shuang Xi cui Yu Feng FAng Ai Bing NIE Chao qun Key Laboratory of Advanced Energy and Power Institute of Engineering Thermophysics Chinese Academy of Sciences University of Chinese Academy of Sciences

Swirl-premixed combustion systems exhibit potential to meet future regulations on pollution emissions. However, combustion induced vortex breakdown(CIVB) flashback is frequently observed in these systems, especially for high hydrogen content fuel. In this study, a swirl-premixed burner with diverging centerbody was used to investigate CIVB flashback based on azimuthal vorticity at mixing zone exit. Through 2D axisymmetric model, it was found that there was a maximal azimuthal vorticity at mixing zone exit for each equivalence ratio. The physical meaning of these maximal azimuthal vorticity values was the minimally required azimuthal vorticity to trigger CIVB flashback. At the same time, the required azimuthal vorticity declined with the increase of equivalence ratio since turbulent burning velocity started to control flashback. Nevertheless, azimuthal vorticity offered by heat release increased with the increase of equivalence ratio, which promoted flame propagating upstream continually.

关键词： azimuthal vorticity mixing zone exit premixed hydrogen flame CIVB flashback

Delivery of CatSper2 siRNA into Rat Sperms by Electroporation Repressed Ca^(2+) Influx During Sperm Hyperactivation

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Agricultural Sciences in China 2011年第12期10卷 1958-1967页

作者： ZHAng Zhen ZHOU Xuan LI Hui-xia cui qun-wei YU Jing WAng Gen-lin College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 PR.China

CatSper is a unique Ca2＋ channel-like protein family exclusively expressed in the testis and sperm, and plays important roles in sperm motility, capacitation, acrosome reaction and sperm-egg interactions. Here we studied the mechanism of regulation of CatSper2-dependent Ca〉 influx, extracellular and intracellular Ca2＋on sperm hyperactivated motility. The siRNA duplexes were transfected into the sperm cells by electroporation （EP） to silence the expression of CatSper2. The results for targeted disruption of CatSper2 showed the highest silence efficiency 77.7% （P〈0.05）, the hyperactivated sperm rate calculated by computer-assisted semen analysis （CASA） analysis of interferenced sperm was significantly lower 11.1% than the control 99.2%. Flow cytometry （FCM） detection of the intracellular Ca2＋ concentration of interferenced sperm was 50 nmol L-t higher than the normal. It was remarkably lower than hyperactivated sperm with 200-1 000 nmol L-1 （P〈0.05）. It was not sufficient to evoke hyperactivation. To trigger hyperactivation, the sperm were incubated in 50 pmol L-t thimerosal or 5 mmol L-1 procaine, it sharply increased the intracellular Ca2＋ via two different channels. CASA and FCM detection indicated that intracellular Ca〉 is required for initiating hyperactivation while extracellular Ca2＋ is essential to maintain the process. We concluded that to mediate sperm hyperactivation, it is essential to inhibit Ca〉peak present with targeted disruption of CatSper2 expression. This provides important prospective for further exploration of signal channel of sperm hyperactivated motility, potential factors for male infertility and provide further reference to the exploration of male contraceptive drug targets of male reproduction.

关键词： sperm hyperactivation motility CatSper2 Ca2+ channel RNAi electroporation (EP)

Focal surface attitude detection for LAMOST

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Research in Astronomy and Astrophysics 2020年第11期20卷 263-272页

作者： Tian-Zhu Hu Yong Zhang Xiang-qun cui Ye-Ping Li Xiu-Shan Pan Ying Fu National Astronomical Observatories/Nanjing Institute of Astronomical Optics&Technology Chinese Academy of ScienceNanjing 210042China CAS Key Laboratory of Astronomical Optics&Technology Nanjing Institute of Astronomical Optics&TechnologyNanjing 210042China University of Chinese Academy of Sciences Beijing 100049China

With telescope apertures becoming larger and larger,the deployment of large-field telescopes is becoming increasingly ***,optical path calibration is necessary to ensure the image quality of large-field and large-diameter *** particular,focal plane attitude calibration is an essential optical path calibration technique that has a direct impact on image *** this paper,a focal plane attitude detection method using eight acquisition cameras is proposed based on the calibration requirements of the wide-field telescope,LAMOST(Large Sky Area Multi-Object Fiber Spectroscopic Telescope).Comparison of simulation and experimental results shows that the detection accuracy of the proposed method can reach 30 *** additional testing and verification,this method could be used to facilitate regular focal plane attitude calibration for LAMOST as well as other large-field telescopes.

关键词： methods data analysis techniques high angular resolution techniques image processing

Endogenous phytohormones and the expression of flowering genes synergistically induce flowering in loquat

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Journal of Integrative Agriculture 2020年第9期19卷 2247-2256页

作者： CHI Zhuo-heng WAng Yong-qing DEng qun-xian ZHAng Hui PAN cui-ping YAng Zhi-wu College of Horticulture Sichuan Agricultural UniversityChengdu 611130P.R.China Sichuan Academy of Forestry Chengdu 610081P.R.China

Flowering is an important process for the reproduction of higher *** to this point,the studies on flowering have mostly focused on the model plant Arabidopsis thaliana,and the flowering mechanism of fruit trees remains mostly *** diversity of the flowering time of loquat(Eriobotrya japonica Lindl.)makes it an ideal material to study the regulation of *** this study,we first observed the inflorescence bud differentiation in two varieties of loquat that had different blooming times(***(***),that blooms in the fall and ***(***×Eriobotrya bengalensis Hook.f.)that blooms in the spring)and found that the starting time of inflorescence bud differentiation and the speed of inflorescence development were responsible for the difference in blooming *** determination of endogenous phytohormones by high performance liquid chromatography(HPLC)indicated that abscisic acid(ABA),zeatin(ZT),and gibberellin(GA3)promoted flowering in loquat,while indole-3-acetic acid(IAA)was mainly involved in inflorescence bud differentiation in Chunhua.A transcription level analysis illustrated that multiple flowering-related genes could respond to different signals,integrate to the TFL1,AP1 and FT genes,and then synergistically regulate flowering in ***,this study provides a new insight into flowering regulation mechanisms in loquat.

关键词： loquat inflorescence bud differentiation flowering endogenous hormones gene expression

Referred Somatic Hyperalgesia Mediates Cardiac Regulation by the Activation of Sympathetic Nerves in a Rat Model of Myocardial Ischemia

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Neuroscience Bulletin 2022年第4期38卷 386-402页

作者： Xiang cui Guang Sun Honglei Cao qun Liu Kun Liu Shuya Wang Bing Zhu Xinyan Gao Department of Physiology Institute of Acupuncture and MoxibustionChina Academy of Chinese Medical SciencesBeijing 100700China Research Center of Traditional Chinese Medicine The Affiliated Hospital of Changchun University of Chinese MedicineChangchunJilin 130021China Department of Cardiology Jining No.1 People’s HospitalJining 272100ShandongChina Department of Needling Manipulation Institute of Acupuncture and MoxibustionChina Academy of Chinese Medical SciencesBeijing 100700China

Myocardial ischemia(MI)causes somatic referred pain and sympathetic hyperactivity,and the role of sensory inputs from referred areas in cardiac function and sympathetic hyperactivity remain ***,in a rat model,we showed that MI not only led to referred mechanical hypersensitivity on the forelimbs and upper back,but also elicited sympathetic sprouting in the skin of the referred area and C8–T6 dorsal root ganglia,and increased cardiac sympathetic tone,indicating sympathetic-sensory ***,intensifying referred hyperalgesic inputs with noxious mechanical,thermal,and electro-stimulation(ES)of the forearm augmented sympathetic hyperactivity and regulated cardiac function,whereas deafferentation of the left brachial plexus diminished *** injection of the α_(2) adrenoceptor(α_(2)AR)antagonist yohimbine and agonist dexmedetomidine in the forearm attenuated the cardiac adjustment by ***,these findings suggest that sensory inputs from the referred pain area contribute to cardiac functional adjustment via peripheral α_(2)AR-mediated sympathetic-sensory coupling.

关键词： Sympathetic-sensory coupling Neuromodulation Referred pain α_(2)adrenoceptor Cardioprotectio

Co-delivery of enzymes and photosensitizers via metal-phenolic network capsules for enhanced photodynamic therapy

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Chinese Chemical Letters 2022年第4期33卷 1917-1922页

作者： Qian Wang Zhiliang Gao Kaijie Zhao Peiyu Zhang Qi-Zhi Zhong qun Yu Shumei Zhai Jiwei cui Key Laboratory of Colloid and Interface Chemistry of the Ministry of Education School of Chemistry and Chemical EngineeringShandong UniversityJi’nan 250100China ARC Centre of Excellence in Convergent Bio-Nano Science and Technology and the Department of Chemical EngineeringThe University of MelbourneParkvilleVIC 3010United States State Key Laboratory of Microbial Technology Shandong UniversityQingdao 266237China

The intrinsic hypoxic tumor microenvironment and limited accumulation of photosensitizers(PSs) result in unsatisfied efficiency of photodynamic therapy(PDT).To enhance the PDT efficiency against solid tumors,a functional oxygen self-supplying and PS-delivering nanosystem is fabricated via the combination of catalase(CAT),chlorin e6(Ce6) and metal-phenolic network(MPN) *** is demonstrated that the CAT encapsulated in the capsules(named CCM capsules) could catalyze the degradation of hydrogen peroxide(H;O;) to produce molecular oxygen(O;),which could be converted into cytotoxicity reactive oxygen species(ROS) by surface-loaded Ce6 under 660 nm laser irradiation,leading to synergistic anticancer effects in vitro and in ***,the application of CCM capsule could be a promising strategy to improve PDT effectiveness.

关键词： Metal-phenolic network(MPN) Photodynamic therapy(PDT) Capsule Oxygen self-supply Drug delivery

A genetic diversity assessment of starch quality traits in rice landraces from the Taihu basin,China

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Journal of Integrative Agriculture 2016年第3期15卷 493-501页

作者： AO Yan XU Yong cui Xiao-fen WAng An TEng Fei SHEN Li-qun LIU Qiao-quan Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Co-Innovation Center for Modern Production Technology of Grain Crops/College of Agriculture Yangzhou UniversityYangzhou 225009P.R.China Suzhou Chien-Shiung Institute of Technology Suzhou 215411P.R.China

There are nearly 1 000 rice landrace varieties in the Taihu basin, China. To assess the genetic diversity of the rice, 24 intragenic molecular markers（representing 17 starch synthesis-related genes） were investigated in 115 Taihu basin rice landraces and 87 improved cultivars simultaneously. The results show that the average genetic diversity and polymorphism information content values of the landraces were higher than those of improved cultivars. In total, 41 and 39 allele combinations（of the 17 genes） were derived from the landraces and improved cultivars, respectively; only two identical allele combinations were found bet ween the two rice variety sources. Cluster analysis, based on the molecular markers, revealed that the rice varieties could be subdivided into five groups and, within these, the japonica improved rice and japonica landrace rice varieties were in two separate groups. According to the quality reference criteria to classify the rice into grades, some of the landraces were found to perform we ll, in terms of starch quality. For example, according to NY /T595-2002 criteria from the Ministry of Agriculture of China, 25 and 33 landraces reached grade 1, in terms of their apparent amylose content and gel consistency. Th e varieties that had outstanding quality could be used as breeding materials for rice quality breeding programs in the future. Our study is useful for future applications, such as genetic diversity studies, the protection of rice variety and improvment of rice quality in breeding programs.

关键词： intragenic molecular marker starch synthesis improved cultivars cluster analysis polymorphism information content

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胞磷胆碱治疗卒中后认知障碍患者的临床研究

中国临床药理学杂志 2024年第19期40卷 2801-2805页

作者：高昶刘燕聂琼王新宇张翠翠济宁医学院精神卫生学院山东济宁272067 济宁医学院研究生处山东济宁272067 济宁医学院临床医学院山东济宁272067 济宁医学院附属医院重症医学三科山东济宁272029

目的观察胞磷胆碱钠片联合石杉碱甲片治疗卒中后认知障碍患者的临床疗效及安全性。方法将卒中后认知障碍患者按队列法分为对照组和试验组。对照组口服石杉碱甲片,每次0.1~0.2 mg,bid;试验组在对照组治疗的基础上,口服胞磷胆碱钠片,每次0... 详细信息

目的观察胞磷胆碱钠片联合石杉碱甲片治疗卒中后认知障碍患者的临床疗效及安全性。方法将卒中后认知障碍患者按队列法分为对照组和试验组。对照组口服石杉碱甲片,每次0.1~0.2 mg,bid;试验组在对照组治疗的基础上,口服胞磷胆碱钠片,每次0.2 g,tid。2组患者均治疗12周。比较2组患者的临床疗效、认知功能[简易智力状态检查量表(MMSE)评分、美国国立卫生研究院卒中量表评分、Barthel指数]、神经功能、日常生活能力以及血清血管内皮生长因子(VEGF)、脑源性神经营养因子(BDNF)水平,并评价安全性。结果试验组和对照组各入组100例。治疗后,试验组和对照组的总有效率分别为92.00%(92例/100例)和75.00%(75例/100例),在统计学上差异有统计学意义(P23.40±2.43)和(19.35±2.51)分,美国国立卫生研究院卒中量表评分分别为(12.25±1.24)和(15.84±1.61)分,Barthel指数分别为(71.14±8.60)和(64.26±8.33)分,VEGF水平分别为(191.52±14.80)和(125.73±11.48)pg·mL^(-1),BDNF水平分别为(9.47±1.59)和(8.01±1.35)ng·mL^(-1),试验组的上述指标与对照组比较,在统计学上差异均有统计学意义(均P0.05)。结论胞磷胆碱钠片联合石杉碱甲片治疗卒中后认知障碍患者的疗效确切,对患者认知功能、神经功能具有明显的改善作用,安全性良好。

关键词：胞磷胆碱钠片石杉碱甲片卒中后认知障碍神经功能血管内皮生长因子脑源性神经营养因子安全性评价