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Rosemary leaf powder improves egg quality, antioxidant status, gut barrier function, and cecal microbiota and metabolites of late-phase laying hens

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Animal nutrition 2024年第2期17卷 325-334页

作者： Lianhua Zhang Junwei Ge fei Gao Min Yang Hui Li fei Xia Hongtong Bai Xiangshu Piao Zhiying Sun Lei Shi Key Laboratory of Plant Resources Institute of Botany Chinese Academy of Sciences China national Botanical Garden State Key Laboratory of Animal nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences College of Pharmacy Shandong University of Traditional Chinese Medicine University of Chinese Academy of Sciences Beijing Jingwa Agricultural Science&Technology Innovation Center State Key Laboratory of Animal nutrition College of Animal Science and Technology China Agricultural University

his study sought to determine the effects of rosemary leaf powder(RP) on laying performance, egg quality, serum indices, gut barrier function, and cecal microbiota and metabolites of late-phase laying hens. A total of 84 “Jing Tint 6” laying hens at 65-week old were randomly divided into 2 groups and fed either a basal diet(COn) or a basal diet supplemented with 0.3% RP. Our study revealed that RP improved the Haugh unit and decreased yolk n-6/n-3 polyunsaturated fatty acid(PUFA) ratio of laying hens,increased serum superoxide dismutase(SOD), jejunal activities of SOD and catalase(CAT), and jejunal zonula occludens-1(ZO-1) expression, as well as decreased serum tumor necrosis factor-a(TnF-a) level and jejunal TnF-a m RnA expression. Rosemary leaf powder markedly enhanced(P ndances of Rikenellaceae, Rikenellaceae＿RC9＿gut＿group, and Turicibacter, tended to promote(P = 0.076)butyrate concentration, and reduced(P ndances of Erysipelatoclostridiaceae, Sutterellaceae, Fusobacteriaceae, Campylobacteraceae, Sutterella, Campylobacter, and Fusobacterium, which were closely linked with Haugh unit, yolk n-6/n-3 PUFA ratio, serum SOD and TnF-a. In addition, RP altered the metabolic functions of cecal microbiota and enhanced the abundances of butyratesynthesizing enzymes, including lysine 2,3-aminomutase, β-lysine 5,6-aminomutase, and 3-oxoacid Co A-transferase. Together, 0.3% RP has the potential to enhance egg quality by partially modulating serum antioxidant status, jejunal barrier function, and cecal microbiota structure and metabolites,indicating that RP could be considered a promising feed additive to promote the production performance of late-phase laying hens.

关键词： Rosemary leaf powder Production Antioxidant status Gut health Microbial community Laying hen

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Hydrolysis mechanism of YbB_(2)C_(2)and the microstructure of the carbon derived from the hydrolysis reaction

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Journal of Materials Science & Technology 2024年第4期171卷 209-221页

作者： Zhihui Li Hao Zhang Jixin Chen Jiemin Wang Xiaohui Wang Jinxing Yang Chao Zhang Zerong Zhang Hongyang Liu fei Huang Meishuan Li fei Li Shenyang national Laboratory for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China School of Materials Science and Engineering University of Science and Technology of ChinaShenyang 110016China Shanghai Key Laboratory for High Temperature Materials and Precision Forming School of Materials Science and EngineeringShanghai Jiao Tong UniversityShanghai 200240China

Carbide-derived carbon(CDC)materials have gained great attention due to the excellent properties for various potential ***,graphite crystal is formed during a room-temperature hydrolysis pro-cess of layered compound YbB_(2)C_(2).The formation mechanism can be demonstrated by a YbB_(2)C_(2)molecular cell:Yb^(3+)acts as a cathode where H_(2)O molecule is reduced to H atom and OH^(−)ion,while(B_(2)C_(2))^(3−)acts as an anode where OH−ion is oxidized to O ***,YbB_(2)C_(2)molecular cell begins to disintegrate,i.e.,Yb^(3+)ion,B and C atoms dissociate from the molecular *** as-produced C atoms combine to form graphite *** initial graphite crystal is a cabbage-like microsphere,and then it gradually disintegrates and transforms into layered *** addition,YbB_(6),Yb_(3)(OH)_(3)n(BO_(3))_((3-n))sol,hydrogen,hydrocarbons,and carbon oxides form *** method provides a general and inexpensive route to obtain carbide-derived graphite crystal.

关键词： Carbide-derived carbon YbB_(2)C_(2) Hydrolysis Molecular cell First-principles calculations

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On the Factors Influencing the High Stretch-Flangeability of a Low-Density 1180 MPa Fe-Mn-Al-C-nbδ-QP Steel

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Acta Metallurgica Sinica(English Letters) 2024年第8期37卷 1291-1300页

作者： Dan‑Dan Cui Peng Chen Peng‑fei Wang Xiao‑Wu Li Department of Materials Physics and Chemistry School of Materials Science and Engineeringand Key Laboratory for Anisotropy and Texture of Materials(Ministry of Education)Northeastern UniversityShenyang 110819China State Key Laboratory of Rolling and Automation Northeastern UniversityShenyang 110819China

Low-densityδ-quenching and partitioning(δ-QP)steels with excellent strength and ductility have been recently ***,there are still rare reports on the formability of δ-QP steels,which are critical for satisfying the manufacture of structural parts during the application in automotive *** the present work,an 1180 MPa Fe–Mn–Al–C–nbδ-QP steel with a high ductility was adopted for the stretch–flangeability ***δ-QP steel was developed by separated quenching and partitioning processes.A good hole expansion ratio(HER)of 34.9±0.9%was obtained in the quenched steel,but it has been further increased to 52.2%by the tempering *** improved stretch–flangeability was attributed to the enhanced austenite stability and deformation *** the one hand,the stability of austenite was increased by carbon partitioning during tempering,which reduced crack possibility via the suppression of the fresh martensite *** the other hand,the tempering treatment released the internal stress caused by martensitic transformation and reduced the difference in strength among different phases,resulting in an increase in the resistance to crack initiation and propagation.

关键词： Hole expansion ratio Low-densityδ-quenching and partitioning(δ-QP)steel Stretch-flangeability Austenite stability Deformation uniformity

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Sensitive and Specific Y-Shaped Ratio Biosensor for Detecting Serum mi R-18a:Potential Early Scanning Tool for non-Small Cell Lung Cancer

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Journal of Analysis and Testing 2024年第2期8卷 237-244页

作者： fei Yu Yue Wang Caiyuan Yu Wei Zhang Xuefeng Bai State Key Laboratory of Quality Research in Chinese Medicines Macao Institute for Applied Research in Medicine and HealthMacao University of Science and TechnologyTaipaMacao999078China Baotou Cancer Hospital Baotou014040Inner MongoliaChina

miR-18a has been identified as a significantly expressed microRnA(miRnA)in non-small cell lung cancer(nSCLC)and plays a vital role in cancer cell transformation,metastasis,and ***,a pair of binary probes from numerous probe pairs based on single nucleotide polymorphism analyses of miR-18a and miR-18b was first designed and screened to develop a Y-shaped ratio biosensor for accurate detection of serum miR-18a in *** special structure of the binary probes combined with the hairpin showed strong specificity for miR-18a,which was confirmed by polyacrylamide gel electrophoresis assay and square wave voltammetry ***,it is beneficial to immobilize single-stranded DnA(ssDnA)probes due to the large specific surface area of nanoporous gold,thereby improving the sensitivity of the *** Y-shaped ratio biosensor exhibited a wide detection range and can quantify the concentration of miR-18a in the range of 10 fmol/L–100 pmol/L,with a limit of detection of 0.211 fmol/L(S/n=3).Moreover,it exhibits excellent detection capabilities in serum samples since the biosensor showed a high selectivity toward the coexistence of miR-18a and ***,the prepared Y-shaped ratio biosensor is a highly sensitive and specific miR-18a detection tool,capable of identifying microscopic amounts of miR-18a in serum samples,providing great potential for early screening of nSCLC.

关键词： nanoporous gold Y-shaped biosensor miR-18a nSCLC

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The response of structure and nitrogen removal function of the biofilm on submerged macrophytes to high ammonium in constructed wetlands

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Journal of Environmental Sciences 2024年第8期142卷 129-141页

作者： Xue Jiang Mengmeng Wang Shunqing Yang Di He fei Fang Liuyan Yang State Key Laboratory of Pollution Control and Resource Reuse School of the EnvironmentNanjing UniversityNanjing 210023China School of Resources and Environment Anqing Normal UniversityAnqing 246133China

The ammonium exceedance discharge from sewage treatment plants has a great risk to the stable operation of subsequent constructed wetlands(CWs).The effects of high ammonium shocks on submerged macrophytes and epiphytic biofilms on the leaves of submerged macrophytes in CWs were rarely mentioned in previous *** this paper,the 16S rRnA sequencing method was used to investigate the variation of the microbial communities in biofilms on the leaves of Vallisneria natans plants while the growth characteristics of *** plants were measured at different initial ammonium *** results demonstrated that the total chlorophyll and soluble sugar synthesis of *** plants decreased by 51.45%and 57.16%,respectively,and malondialdehyde content increased threefold after8 days if the initial nH_(4)^(+)-n concentration was more than 5 mg/*** density,bacterial quantity,dissolved oxygen,and pH increased with high ammonium *** average removal efficiencies of total nitrogen and nH_(4)^(+)-n reached 73.26%and 83.94%,*** heat map and relative abundance analysis represented that the relative abundances of phyla Proteobacteria,Cyanobacteria,and Bacteroidetes *** numbers of autotrophic nitrifiers and heterotrophic nitrification aerobic denitrification(HnAD)bacteria expanded in *** particular,HnAD bacteria of Flavobacterium,Hydrogenophaga,Acidovorax,Acinetobacter,Pseudomonas,Aeromonas,and Azospira had higher abundances than autotrophic nitrifiers because there were organic matters secreted from declining leaves of *** *** analysis of the nitrogen metabolic pathway showed aerobic denitrification was the main nitrogen removal ***,the nitrification and denitrification bacterial communities increased in epiphytic biofilms on submerged macrophytes in constructed wetlands while submerged macrophytes declined under ammonium shock loading.

关键词： Ammonium shock loading Biofilm Autotrophic nitrifiers Heterotrophic nitrification aerobic denitrification bacteria Submerged macrophyte

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Effect of Frequency on Degradation in BOPP Films Under Repetitively Pulsed Voltage

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CSEE Journal of Power and Energy Systems 2024年第3期10卷 1280-1290页

作者： Chuansheng Zhang Yu Feng fei Kong Bangdou Huang Cheng Zhang Tao Shao Beijing International S&T Cooperation Base for Plasma Science and Energy Conversion Institute of Electrical EngineeringChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China

Operating conditions of film capacitors are complex,and the problem of film insulation failure caused by repetitively pulsed voltage is becoming ever *** of the film under repetitively pulsed voltage cannot be accurately evaluated by the average breakdown electric *** this paper,the effects of pulsed electric field and pulse repetition frequency on the breakdown in biaxially oriented polypropylene(BOPP)films are *** phases of BOPP degradation are proposed based on the voltage amplitude,i.e.,maintenance(M),decline(D),and near-zero(n).Evolution of the BOPP film from degradation to breakdown at different frequencies is ***,transition of discharge mode and elemental composition of the film are *** results show continuous heat generation under repetitive microsecond pulses is the dominant factor for degradation of BOPP *** number of applied pulses and the repetitive stressing time decrease exponentially with increase of *** research can be contributed to the safe and reliable operation of capacitors.

关键词： Aging breakdown film capacitor polypropylene repetitive pulse

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Experimental and first-principles calculation of Tin growth mechanism on CeAlO_(3) surface in steel

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Journal of Iron and Steel Research International 2024年第2期31卷 452-463页

作者： fei-hu He Jun Peng Fang Zhang Yong-bin Wang Hong-tao Chang School of Materials and Metallurgy Inner Mongolia University of Science and TechnologyBaotou014010Inner MongoliaChina

Regular Tin is harmful to the toughness of steel,and its shape and size need to be *** the behavior of Tin precipitation on CeAlO_(3) surfaces is critical for controlling the morphology and formation process of CeAlO_(3)–Tin composite inclusions in the *** results showed that Tin had a square morphology on the CeAlO3 surface,and electron backscatter diffraction phase identification results revealed the orientation relationship between CeAlO_(3) and Tin as follows:(001)_(CeAlO_(3))//(110)_(Tin),(100)_(CeAlO_(3))/(001)_(Tin),and[010]_(CeAlO_(3))/[110]_(Tin).The CeAlO3 crystal structure was studied using the first-principles calculation method,and the adsorption and growth process of Tin on the CeAlO_(3) surface were investigated from the atomic *** calculation results indicate that there is no metallic bonding present in the CeAlO_(3) *** the low-index crystal planes of CeAlO_(3),the(110)planes terminated with O and CeAlO have the highest and lowest surface energies,respectively,with values of 0.373 and 0.051 eV/Å^(2).On the high surface energy plane of CeAlO_(3),the Tin atomic permutation structure is consistent with the arrangement of Ti and n atoms in Tin(100)or(110).For the low surface energy plane of CeAlO_(3),the Ti and n atoms are arranged in a ring-like structure.

关键词： CeAlO_(3)-Tin inclusion Orientation relationship Crystal structure First principle Surface energy

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Fe-n_(x) sites coupled with core-shell FeS@C nanoparticles to boost the oxygen catalysis for rechargeable Zn-air batteries

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Journal of Energy Chemistry 2024年第3期90卷 565-577,I0013页

作者： Katam Srinivas Zhuo Chen Anran Chen fei Ma Ming-qiang Zhu Yuanfu Chen School of Integrated Circuit Science and Engineering and State Key Laboratory of Electronic Thin Films and Integrated DevicesUniversity of Electronic Science and Technology of ChinaChengdu 610054SichuanChina College of Mechanical and Electronic Engineering Northwest A&F UniversityYangling 712100ShaanxiChina School of Materials and Energy Yunnan UniversityKunming 650091YunnanChina

The development of efficient single-atom catalysts(SACs) for the oxygen reduction reaction(ORR)remains a formidable challenge,primarily due to the symmetric charge distribution of metal singleatom sites(M-n_(4)).To address such issue,herein,Fe-n_(x) sites coupled synergistic catalysts fabrication strategy is presented to break the uniform electronic distribution,thus enhancing the intrinsic catalytic ***,atomically dispersed Fe-n_(x) sites supported on n/S-doped mesoporous carbon(nSC)coupled with FeS@C core-shell nanoparticles(FAS-nSC@950) is synthesized by a facile hydrothermal reaction and subsequent *** to the presence of an in situ-grown conductive graphitic layer(shell),the FeS nanoparticles(core) effectively adjust the electronic structure of single-atom Fe sites and facilitate the ORR kinetics via short/long-range coupling ***,FAS-nSC@950displays a more positive half-wave potential(E_(1/2)) of 0.871 V with a significantly boosted ORR kinetics(Tafel slope=52.2 mV dec^(-1)),outpacing the commercial Pt/C(E_(1/2)=0.84 V and Tafel slope=54.6 mV dec^(-1)).As a bifunctional electrocatalyst,it displays a smaller bifunctional activity parameter(ΔE) of 0.673 V,surpassing the Pt/C-RuO_(2) combination(ΔE=0.724 V).Besides,the FAS-nSC@950-based zincair battery(ZAB) displays superior power density,specific capacity,and long-term cycling performance to the Pt/C-Ir/C-based *** work significantly contributes to the field by offering a promising strategy to enhance the catalytic activity of SACs for ORR,with potential implications for energy conversion and storage technologies.

关键词： Fe-n_(x)sites Core-shell FeS@C Synergistic interactions Oxygen reduction reaction Zn-air battery

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Copper-based nanomaterials as peroxidase candidates for intelligent colorimetric detection and antibacterial applications

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Particuology 2024年第1期84卷 126-135页

作者： Yanyan Huang Huimin Zhong Cong Jiang Jiahui Yang Jingwen Zhang fei Zhao Chaoqun Liu College of Light Industry and Food Engineering Nanjing Forestry UniversityNanjing210037China College of Pharmacy Institutes of Environment and MedicineHenan UniversityKaifeng475004China

With natural polyphenol compound tannic acid(TA)as the ingredient,copper tannate(CuTA)nanolayer material was formed by self-assembly under the conditions of heating and copper *** ion and tannic acid could coordinate to form Cu-O-C structure which was similar to the Fe-n-C structure of natural heme *** addition,the obtained CuTA nanomaterial possessed excellent peroxidase-like catalytic activity and *** on this excellent enzymatic activity,CuTA nanomaterials could be used for colorimetric detection of hydrogen peroxide with a smartphone and decomposition of organic *** on the structural characteristics and catalytic activity,the copper tannate nanozymes could also serve as potential antibacterial reagents to effectively inhibit the growth of gram-negative and positive bacteria in a low hydrogen peroxide *** work may promote the exploration of novel peroxidase mimetics and broaden the applications of nanozymes.

关键词： nanozyme Colorimetric detection Smartphone Antibacteria

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Yolk free egg substitute improves the serum phospholipid profile of mice with metabolic syndrome based on lipidomic analysis

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Food Science and Human Wellness 2024年第1期13卷 482-493页

作者： Zhihui Yu Lingyu Fan fei Tai Lixin Zhang Xiaoyu Zhang Yisheng Chen College of Food Science and Engineering Shanxi Agricultural UniversityTaigu 030801China Insitute of Food nutrition and Safety Shanxi Agricultural UniversityTaiyuan 030031China Shanxi Key Laboratory of Edible Fungi for Loess Plateau Taigu 030801China

In this study,the impacts of egg consumption on mice model of metabolic syndrome(Met S)were comparatively *** were divided into five groups(n=8):normal diet group(nD),high-fat diet group(HFD),HFD with whole egg group(WE),HFD with free-yolk egg substitute group(YFES),and HFD with lovastatin group(Lov).Main biochemical indexes and a non-targeted lipidomic analysis were employed to insight the lipid profile changes in *** was revealed that WE could significantly improve serum biochemical indexes by reducing body weight,low-density lipoprotein cholesterol(LDL-C)and total cholesterol(TC),while increasing high-density lipoprotein *** exhibited remarkably better performance in increasing phosphatidylglycerol and phosphatidic acids,while decreasing phosphatidylinositol than WE.A total of 50 differential lipids biomarkers tightly related to glycerophospholipids metabolism were screened *** C18:2 and C12:1,SM(d18:0/12:0),and SM(d18:1/14:1)were significantly upregulated in YFES compared to *** reduced expression of SREBP-1c and Cpt1a,while did not affect the expression of PPAR-α.Sphingomyelin biomarkers were positively related to the TC(|r|>0.6),while PPAR-αwas negatively correlated with triglyceride and LDL-C *** sum up,YFES attenuated HFD-induced Met S by improving the serum phospholipids,which account for its modulation of glycerophospholipid metabolism.

关键词： Metabolic syndrome Whole egg Yolk free egg substitute Serum Lipidomics

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