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Synthesis of GoldMag particles with assembled structure and their applications in immunoassay

Science China Chemistry 2006年第6期49卷 534-540页

作者： CUI Yali1, ZHANG Lianying1, SU Jing1, ZHANG Caifeng3, LI Qi2. CUI Ting3, JIN Boquan2.& CHEN Chao1,3 1. Biochip research and Development center, Northwest University ({2. engineering research center for Miniaturized Detection system), Xi’an 710069, China 2. Department of Immunology, The Fourth Military Medical University, Xi’an 710032. China 3. Shaanxi Lifegen Co., Ltd., Xi’an 710069, China 1. Biochip research and Development center Northwest University (National Engineering Research Center for Miniaturized Detection System) Xi’an 710069 China 3. Shaanxi Lifegen Co. Ltd. Xi’an 710069 China 2. Department of Immunology The Fourth Military Medical University Xi’an 710032 China

Micrometer-sized Fe3O4 particles and nano-sized gold particles were first synthesized by methods of self-aggregation of surface-chemically modified Fe3O4 nanoparticles and citrate reduction of the Au3+ to Au0, respectively. Interaction between these two types of particles resulted in the assembly of nano-sized gold particles on the surface of the micrometer-sized Fe3O4 particles, forming an assembled structure with the Fe3O4 core particles around which are attached nano-sized gold parti- cles. The Fe3O4/Au structure is named GoldMag particles with assembled structure. The synthetic process, structure, and magnetic property of the GoldMag particles were analyzed. GoldMag particles with assembled structure have an irregular shape, rough surface with a diameter of 2.3 μm. These particles exhibit the superparamagnetic property with saturated magnetization of 41 A·m2.kg. In a single step, antibodies could be readily immobilized onto the surface of the particles with a high binding capacity. The GoldMag particles can be used as a novel carrier in immunoassays. The maximum quantity of human IgG immobilized onto GoldMag particles was 330 μg/mg. In order to validate the quality of the GoldMag particles as immunoassay carriers, an immunoassay system was used. The relative amount of immobilized human IgG was measured by HRP-labeled anti human IgG. The coefficient of variation within parallel samples of each group was below 6% and the coefficient of variation of means between five groups carried out separately was below 7%. Based on the sandwich method, the Hepatitis B surface antigen (HBsAg) and interleukin-8 (IL-8) were also analyzed by qualitative and quantitative detection, respectively. The result indicated that the GoldMag particles with assembled structure were an ideal carrier in immunoassay.

关键词： GoldMag particles with assembled structure, synthesis, carrier, immunoassay.

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Detection of subcritical crack propagation for concrete dams

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Science China(technological Sciences) 2009年第12期52卷 3654-3660页

作者： BAO TengFei1 ,2.YU Hong 1,2.1College of Water Conservancy&Hydropower engineering,Hohai University,Nanjing 2.0098,China 2.1} engineering research center of Water Resources Efficient Utilization and engineering Safety,Nanjing 2.0098,China College of Water Conservancy & Hydropower engineering Hohai University Nanjing 210098 China national engineering {2. center of Water Resources Efficient Utilization and engineering Safety Nanjing 210098 China

Subcritical propagation of cracks is a warning sign of *** such propagation is detected at an early stage,timely maintenance measures can be taken to prevent the failure of *** detect the subcritical propagation of a crack,the crack needs to be monitored continuously in a long term, which is not realistic under certain ***,cracks in concrete dams can be monitored continuously by dam monitoring to offer possible detection for subcritical *** this paper, with measured crack openings from dam monitoring,a state equation for characterizing crack development is established based on the grey system *** relation between the stability of the equation and the subcritical crack propagation is investigated,then a criterion is proposed for detecting subcritical *** example demonstrates the validity of the criterion and its potential for practical application.

关键词： crack subcritical propagation criterion concrete

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Assessment model of dam operation risk based on monitoring data

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Science China(technological Sciences) 2007年第z1期50卷 144-152页

作者： WU ZhongRu1,2., SU HuaiZhi1,2.& GUO HaiQing1 1 College of Water Conservancy and Hydropower engineering, Hohai University, Nanjing 2.0098, China 2.national engineering research center of Water Resources Efficient Utilization and engineering Safety, Nanjing 2.0098, China 1. College of Water Conservancy and Hydropower engineering Hohai University Nanjing 210098 China 2. national engineering {2. center of Water Resources Efficient Utilization and engineering Safety Nanjing 210098 China

Although the dams produce remarkable social and economic benefits, the threat made by unsafe dams to the life and property of people who live in the lower river area is un-negligible. Based on the monitoring data which reflect the safety condition of dams, the risk degree concept is proposed and the analysis system and model for evaluating risk degree (rate) are established in this paper by combining the reliability theory and field monitoring data. The analysis method for risk degree is presented based on Bayesian approach. A five-grade risk degree system for dam operation risk and corresponding risk degree is put forward according to the safety condition of dams. The operation risks of four cascade dams on some river are analyzed by the model and approach presented here and the result is adopted by the owner.

关键词： dam monitoring data operation risk degree analysis model

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research on stability of the accumulated rock-soil body of reservoir bank under rainfall condition

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Science China(technological Sciences) 2009年第9期52卷 2528-2535页

作者： GU ChongShi1,2.WU HaiZhen1,2.& SU HuaiZhi1,2.1 College of Water Resources and Hydropower engineering,Hohai University,Nanjing 2.0098,China 2.national engineering research center of Water Resources Efficient Utilization and engineering Safety,Hohai University,Nanjing 2.0098,China 3 Jiangxi Water Conservancy research Institute,Nanchang 33002.,China 1. College of Water Resources and Hydropower engineering Hohai University Nanjing 210098 China 2. national engineering {2. center of Water Resources Efficient Utilization and engineering Safety Hohai University Nanjing 210098 China 3. Jiangxi Water Conservancy research Institute Nanchang 330029 China

The shear strength parameters property of rock-soil aggregates in embankment slope of reservoir,that is,the relationship between cohesion and gravel content,between friction angle and gravel content,and the relationship between cohesion and water content,between friction angle and water content,is studied based on the direct shear test results,the shear strength change law of the rock-soil aggregates is given,and the unsaturated shear strength formulation of rock-soil aggregates that could consider suction and saturation degree influence is put forward in this paper,through which the sliding or failure physical mechanism of this type of slope under the condition of rainfall infiltration is studied. Also the 3D unsteady saturated-unsaturated seepage field and its FEM resolving mode are established based on the analysis of the slope rainfall infiltration process. Case study with this method indicates that the minimum safety factor of the accumulated rock-soil aggregates dose not arrive at the moment of rainfall cessation,but appears several hours after the rainfall cessation,this phenomenon is in accordance with the practical slope engineering's failure process and could explain appropriately the slope failure caused by rainfall infiltration. research results in this paper have an important reference value for the research on stability of the accumulated rock-soil aggregates in embankment slope of reservoir,and can enrich the stability analysis method and relevant theory of reservoir embankment slope.

关键词： reservoir bank slope rock-soil aggregates rainfall infiltration saturated/unsaturated seepage

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Multivariate analysis in dam monitoring data with PCA

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Science China(technological Sciences) 2010年第4期53卷 1088-1097页

作者： YU Hong1,2.WU ZhongRu1,2.BAO TengFei1,2.& ZHANG Lan1,2.1 State Key Laboratory of Hydrology-Water Resources and Hydraulic engineering,Hohai University,Nanjing 2.0098,China 2.national engineering research center of Water Resources Efficient Utilization and engineering Safety,Hohai University,Nanjing 2.0098,China 1. State Key Laboratory of Hydrology-Water Resources and Hydraulic engineering Hohai University Nanjing 210098 China2. National Engineering Research Center of Water Resources Efficient Utilization and Engineering Safety Hohai University Nanjing 210098 China

Given the limitation of traditional univariate analysis method in processing the multicollinearity of dam monitoring data,this paper reconstructs the multivariate response variables by introducing principal component analysis(PCA) method,explores the ways of determining principal components(PCs),and extracts a few PCs that have major influence on data *** steady observation series,a control field for the whole observation values has been established based upon PCA;for unsteady observation series that have significant tendency,a control field for the future observation values has been constructed according to PC statistical predication *** methods have already been applied to an actual project and the results showed that data interpretation method with PCA can not only realize data reduction,lower data redundancy,and reduce noise and false alarm rate,but also be effective to data analysis,having a broad application prospect.

关键词： dam safety monitoring multivariate response variables principal component analysis data reduction

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Review on hidden trouble detection and health diagnosis of hydraulic concrete structures

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Science China(technological Sciences) 2007年第z1期50卷 34-50页

作者： WU ZhongRu1,2., LI Ji1, GU ChongShi1,2.& SU HuaiZhi1,2.1 College of Water Conservancy and Hydropower engineering, Hohai University, Nanjing 2.0098, China 2.national engineering research center of Water Resources Efficient Utilization and engineering Safety, Nanjing 2.0098, China College of Water Conservancy and Hydropower engineering Hohai University Nanjing 210098 China national engineering {2. center of Water Resources Efficient Utilization and engineering Safety Nanjing 210098 China

A large number of hydraulic concrete structures have hidden defects such as cracks, erosion, freeze and thaw, thermal fatigue, carbonization. These hidden defects seriously affect the strength, stability and durability of structures. These problems are studied mainly by single monitoring or diagnosis methods at present. The integration of multiple monitoring and diagnosis methods is not applied widely. Besides, the analysis theory on these problems is not developed very well. The systemic study on the aging mechanism of hydraulic concrete structures, time- variation model and health diagnosis is still not enough. The support for engineering practice is limited. Aimed at these major scientific and {2. problems and combined with specific projects, study on detection of hidden defects and health diagnosis of hydraulic concrete structure has been carried out. This study includes the following content: field non-destructive examination of hidden defects of hydraulic concrete structures, seepage detection, the construction of in-situ sensing system, the combination of field detection and in-situ monitoring, the mechanism of crack, freeze and thaw, erosion and carbonization of hydraulic concrete structure, mechanism of combination aging; time-variation model of hydraulic concrete structure, theories and methods for health diagnosis of hydraulic concrete structures.

关键词： hydraulic concrete structure hidden trouble detection health diagnosis

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Asymptotic Behavior of a Non-Local Hyperbolic Equation Modelling Ohmic Heating

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Journal of Mathematical research with Applications 2012年第4期32卷 476-484页

作者： Xianchao WANG 1,,Chengshun JIANG 2.1.national digital {2. system engineering & technological R & D Centre,Henan 450002.P.R.China 2.Wuhan College,Zhongnan University of Economics and Law,Hubei 430079,P.R.China national digital {2. system engineering & technological R & D Centre Henan 450002 Wuhan College Zhongnan University of Economics and Law Hubei 430079

In this paper,the asymptotic behavior of a non-local hyperbolic problem modelling Ohmic heating is *** is found that the behavior of the solution of the hyperbolic problem only has three cases:the solution is globally bounded and the unique steady state is globally asymptotically stable;the solution is infinite when t→∞;the solution blows *** the solution blows up,the blow-up is uniform on any compact subsets of(0,1] and the blow-up rate is lim t → T*-u(x,t)(T*-t)1/α+βp-1=(α+βp-1/1-α)1/1-α-βp,where T* is the blow-up time.

关键词： non-local hyperbolic equation asymptotical behavior blow-up blow-up rate.

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Risk analysis model for landslide mass of high slope in dam area

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Science China(technological Sciences) 2008年第S2期51卷 25-31页

作者： GU ChongShi1,2.HE XianFeng3,4,WU ZhongRu1,2.& SU HuaiZhi1,2.1 College of Water Conservancy and Hydropower engineering,Hohai University,Nanjing 2.0098,China 2.national engineering research center of Water Resources Efficient Utilization and engineering Safety,Nanjing 2.0098,China 3 Yellow River Institute of Hydraulic research,Zhengzhou 450003,China 4 Embankment Security Defect Prevention and Control engineering research center of MWR,Zhengzhou 450003,China 1. College of Water Conservancy and Hydropower engineering Hohai University Nanjing 210098 China 2. national engineering {2. center of Water Resources Efficient Utilization and engineering Safety Nanjing 210098 China 3. Yellow River Institute of Hydraulic research Zhengzhou 450003 China 4. Embankment Security Defect Prevention and Control engineering research {2. of MWR Zhengzhou 450003 China

In order to identify the threat of reservoir bank landslide to dam safety and reduce the risk of dam failure,impact factors of the landslide are *** concept of landslide risk degree is put forward to measure the threat of landslide to dam *** monotony of the landslide risk assessment function which changes with different affecting factors is analyzed *** on gene automatic programming,a data mining model of the landslide risk assessment function is constructed combined with impact factors of reservoir bank landslide on dam *** the assessment function of landslide risk has been excavated and established according to practical project information,which provides a valuable reference for analyzing the impact of landslide on dam risk.

关键词： landslide dam failure risk landslide risk

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Study on Transformation of CaO-Al_2._3-SiO_2.MgO system Inclusions in ZG06Cr13Ni4Mo Stainless Steel

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Journal of Iron and Steel research International 2011年第S2期18卷 341-346页

作者： LIU Jian-hui1,3, DONG Han1,3, ZHU Rong2. LI Shi-qi2.(1. Institute for Structural Materials,Central Iron and Steel research Institute, Beijing 100081,China 2. School of Metallurgical and Ecological engineering,University of Science and Technology Beijing, Beijing 100083,China 3. {2. engineering research center of Advanced Steel Technology, Beijing 100081,China) Cent Iron & Steel Res Inst Inst Struct Mat Beijing 100081 Peoples R China Univ Sci & Technol Beijing Sch Met & Ecol Engn Beijing 100083 Peoples R China Natl Engn Res Ctr Adv Steel Technol Beijing 100081 Peoples R China

This paper aims at generally studying on the inclusions of ZGG06Cr13Ni4Mo ultra-low carbon stainless steel used for large scale hydraulic turbine sets. On the basis of analysis the industrial test samples, thermodynamic software FactSage is used to study inclusions transformation process in CaO-SiO2.Al2.3-MgO system while stainless steel is been smelting. The results illustrate the inclusions melting points and plasticity variation law. The reasonable inclusions plasticity controlling area is found out in low melting point. The melting point is up to 12.0℃ While MgO weight concentration is 10% in CaO-SiO2.Al2.3-MgO system, SiO2. Al2.3 and CaO in inclusions are in weight concentration range of 50-70%, 10-2.% and 10-40%, respectively. For purpose of gaining low melting point inclusions, silicon alumina radio in deoxidant should be controlled between 2.5 for ultra-low carbon stainless steel refining in industry.

关键词： ZG06Cr13Ni4Mo stainless steel low melting point inclusion thermodynamic calculation

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SIMULATION system FOR FIVE-AXIS NC MACHINING USING GENERAL CUTTING TOOL

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Chinese Journal of Mechanical engineering 2001年第2期14卷 144-150页

作者： Wang Chenpei Zhou Yunfei Zhou Yanhong Zhou Ji national NC system {2. research center, Huazhong University of Science and Technology 华中科技大学数控技术所湖北

A simulation system for five axis NC machining using general cutting tools is presented This system differs from other simulation system in that it not only focuses on the geometric simulation but also focuses on the collision detection which is usually not included in NC machining simulation Besides all of these, estimating cutting forces is also discussed In order to obtain high efficiency, all algorithms use swept volume modeling technique, so the simulation system is compact and can be performed efficiently

关键词： Simulation Envelope Z buffer structure Octree

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