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Sampling Survey and Identification of Races of Soybean Cyst Nematode (Heterodera glycines Ichinohe) in Huang-Huai Valleys

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Journal of Integrative Agriculture 2006年第8期5卷 615-621页

作者： LU Wei-guo1, 2. GAI Jun-yi1 and LI Wei-dong2.1 Soybean Research institute of Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 2.0095, P.R.China 2.institute of Cotton and {2. crops, Henan Academy of Agricultural Sciences, Zhengzhou 450002. P.R.China Soybean Research institute of Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing 210095 P.R.China institute of Cotton and oil {2. Henan Academy of Agricultural Sciences Zhengzhou 450002 P.R.China

Soybean cyst nematode (SCN Heterodera glycines Ichinohe) is one of the most important nationwide soybean diseases in China. A total of 38 soil specimens or locations in the area was sampled and tested for SCN races during 2.01-2.03 for the inspection of race distribution in Huang-Huai Valleys. A map of race distribution was constructed according to the data from both the present study and the published reports cited. Three areas, namely, the area of southeast to Jinan in Shangdong Province; the area of northern Henan Province and its border region to south of Hebei Province; and the area of Luohe, Zhoukou of Henan Province and Fuyang of Anhui Province mainly infested with Race 1 were identified. Race 4 was predominant in Shanxi Province, Beijing and the adjacent area of Henan, Shandong, and Anhui provinces, and the delta of Huanghe River in Shandong Province. Race 2.was mainly found in Liaocheng, Dezhou of Shangdong Province and Shijiazhuang of Hebei Province, and Jiaozuo and Huojia of Henan Province. Race 7 was distributed in the west part of Jiaodong Peninsula of Shandong Province and Kaifeng, Huaxian, Wenxian of Henan Province. Race 5 was found and scattered in Hebei and Henan Province. Race 9 was found in Shangqiu of Henan Province, which was reported for the first time in China. It can be seen that Race 1 and Race 4 were the two predominant races in Huang-Huai Valleys, and that research should focus on developing resistant cultivars of these races. There might exist other races in an area with some predominant races. The race substitution in the past decade was not obviously found, therefore, the results should be meaningful to future breeding for resistance to SCN in Huang-Huai Valleys.

关键词： soybean (Glycine max (L.) Merr.) Huang-Huai Valleys cyst nematode (Heterodera glycines Ichinohe) race

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Cloning and identification of Mam1 in Brassica napus

Cloning and identification of Mam1 in Brassica napus

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湖北省遗传学会第八次代表大会暨学术讨论会

作者： Xiaoxia Mei~(1,2.,Junyan Huang~2.Shengyi Liu~(2.) (1.HuBei Uiversity,Wuhan 430062.2.oil crops Research {2.,Chinese Academy of Agricultural Sciences,Wuhan.430062.

Glucosinolates are a group of sulfur-containing secondary metabolites derived from glucose and various amino acids,which occur almost exclusively in members of the *** majority of glucosinolates in Arabidopsis thaliana and many other species of the Brassicaceae are formed from methionine,involving a three-step chain-elongation *** first reaction of the cycle is catalyzed by a methylthioalkylmalate synthase encoded by a gene MAM1. Glucosinolates itself,located in cytoplasm,is a stable compound,but it can degraded by myrosinases released from vacuole and generate a variety of degradation *** plant organs was damaged;they get in touch with each other and lead to the hydrolysis of *** degradation of glucosinolates produces a lot of metabolites,such as *** smell of isothiocyanate has a very strong attraction to many insects,and so we can use this biological specialty in biological control of the crop insect and pests. In this study,the cDNA and DNA sequences of MAM1 gene in Brassica napus were cloned with Genome Walking method and named *** analysis indicated that BnMAM1 shared high homology to MAM1 from Arabidopsis thaliana(84%identity in nucleotides sequence and 80.3%identity in amino acids sequence).We constructed a BnMAM1 overexpression vector(with a herbicide resistant gene bar),then transformed it into *** by a Agrobactierum-medicated floral dip *** identify MAM1 activity in transformed lines and investigate whether transgenic plants are more attractive to the insects,we will detect glucosinolates and isothiocyanate content in transgenic plants and do behavioral assays with different insects.

关键词： MAM1 gene glucosinolates isothiocyanate pest control

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Molecular Analysis and Expression Patterns of Four 14-3-3 Genes from Brassica napus L.

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Agricultural Sciences in China 2010年第7期9卷 942-950页

作者： ZHAN Gao-miao,TONG Jin,WANG Han-zhong and HUA Wei Key Laboratory for Biological Sciences of oil crops,Ministry of Agriculture/oil crops Research institute,Chinese Academy of Agricultural Sciences,Wuhan 430062.P.R.China Key Laboratory for Biological Sciences of oil crops Ministry of Agriculture/Oil Crops Research Institute Chinese Academy of Agricultural Sciences Wuhan 430062 P.R. China

In eukaryotes,14-3-3 proteins constitute a family of ubiquitous regulatory *** play very important roles in many cell ***,their enconding genes and roles in plants remain to be *** this paper,four 14-3-3 genes from Brassica napus *** obtained by randomly sequencing a full-length cDNA library and named as Bn1433-1-4 *** phylogenetic comparison with Arabidopsis 14-3-3 family showed that Bn1433-1 and Bn1433-2.belonged to Episilon group while Bn1433-3 and Bn1433-4 belonged to non-Episilon *** transcript levels of four Bn1433 genes were analyzed in different organs,various stress conditions and some hormone treatments by real-time *** result showed that all the Bn1433s were expressed constitutively in roots,stems,leaves and immature seeds except that Bn1433-4 exhibited strong expression in immature seeds of 14 and 2. DAF（day after flowering）.By the analysis of real-time PCR,different expression patterns of Bn1433s were studied in various stress conditions and hormone *** was suggested that the functions of Bn1433s are diverse and they are involved in the regulation of various stresses and hormones treatments.

关键词： 14-3-3 expression pattem abiotic stress Brassica napus L.

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Embolism Occurrence in Relation to Water Supply in Xylem of Winter Wheat Leaves

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Journal of Integrative Agriculture 2004年第4期 30-37页

作者： JIA Xiu-ling1,2.MA Rui-kun1,ZHANG Quan-guo1 and LI Guang-min1institute of Cereal and oil {2.,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050031,P.R.China2.ebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050051,P.R.China instituteofCerealandoil{2. HebeiAcademyofAgriculturalandForestrySciencesShijiazhuang050031P.R.China

Embolism occurrence in the xylem of leaves was investigated in field-grown winter wheat(cv.9195) under droughted and irrigated conditions by ultrasonic acoustic emission (AE)monitoring with drought stress monitor (Model 4615, Physical Acoustics Corp., USA) inthe growing seasons of 1998-2.00. The measurements have revealed a pattern of 3 activeAE periods for daily AE signals. The 1st active period generally occurred between 10:00-11:00, the 2.d was often observed around 18:00, and the 3rd was at about midnight, AEsusually ceased after 0:00-2.00. AE rates during these periods varied with soil moisture,weather conditions and growth stages, etc. as shown in Fig. 1. The 3rd one was occasionallymissing on severe drought soils. Irrigation often led immediately to higher AE rates overthe ensued 2.d period. Droughted plants having greater amount of 2. h AEs showed higherdegrees of embolism than the irrigated ones during early jointing stage. However, thetotal of 2. h AEs of droughted wheat decreased by 66 and 82. compared with that irrigatedat middle and late stages respectively. This phenomenon was here termed "AE attenuation",which was regarded as an implication of incomplete embolism refilling overnight due tosevere soil moisture depletion. Considerable amount of AE signals were also recordedfrom plants on wet soils. It is suggested by the authors that cavitations and embolismmight be a physiological response to the daily rapid establishment of water potentialgradient within xylem system in the morning, rather than merely a phenomenon resultingfrom water stress.

关键词： Embolism Occurrence in Relation to Water Supply in Xylem of Winter Wheat Leaves

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RESEARCH OF HERBICIDES SCREENING,THRESHOLD ON BROMUS JAPONICUS *** AEGILOPS TAUSCHII *** SAFETY TO WINTER WHEAT

RESEARCH OF HERBICIDES SCREENING,THRESHOLD ON BROMUS JAPONIC...

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中国第三届植物化感作用学术研讨会、第八届全国杂草科学大会、联合国粮农组织——中国“水稻化感作用论坛”

作者： Bing-hua LI~(1,2.,Gui-qi WANG~2.Li-jun SU~2.Cui-qin FAN~2.and Jin-gao DONG ~1 1 College of biology science,AUH,Baoding 071001,2.institute of Cereal and oil {2., HAAFS,Shijiazhuang 050031,P.R.China

The experiments researched fenoxaprop-ethyl,isoproturon,mesosulfuron and flucarbazone-sodium on the serious weeds,Bromus japonicus *** Aegilops tauschii Coss. in different leave stage at the green *** results showed t... 详细信息

关键词： winter wheat herbicide Bromus japonicus Thunb. Aegilops tauschii Coss. control threshold

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Inhibtion of Brassica napus Bn5 Gene Expression Enhances Susceptibility to the Viruses

Inhibtion of Brassica napus Bn5 Gene Expression Enhances Sus...

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中国植物病理学会2.08年学术年会

作者： Cai Li~(1,2. Hou Mingsheng~1 Liu Shengyi~2.1. Department of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070,China 2..The Key Lab. of Genetic Improvement for oil crops, the Ministry of Agriculture, P R. China, oil crops Research institute, Chinese Academy of Agricultural Sciences, Wuhan 430062. China

A gene with putative function for disease resistance was selected from oilseed rape UniGene library and named as *** RNA interference vector of Bn5 gene was constructed and transformed into *** L. by using the pollen ...

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Effects of different soil moisture and nutrient regimes on photosynthesis and protective enzyme of oil palm seedling

Effects of different soil moisture and nutrient regimes on p...

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2.11全国植物生物学研讨会

作者： Cao HongXing~(1,2.,Sun ChengXu~(1,2.,Lei XinTao~(1,2.,Xiao Yong~(1,2. 1 Coconut Research institute,Chinese Academy of Tropical Agricultural Sciences,Wenchang,Hainan 571339,China 2.Hainan Key Laboratory of Tropical oil {2. Biology Wen chang,Hainan 571339,China

The aim of this paper was to investigated the photosynthesis and peroxidases response to two watering regimes(well-watered and water-stress conditions) and to two nutrient regimes(with or without fertilization) of oil *** net photosynthesis rate(Pn) and transpiration rate(Tr)of different treatments with similar two-peak and single-peak changing tendency,*** treatments showed the lowest photosynthesis,transpiration rate when the comparatively higher air temperature light density and intercellular CO concentration at 12.00-13:***

关键词： photosynthesis rate transpiration rate physiology response water stress nutrient stress

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DETECTING MOISTURE CONTENT OF SOUTHERN Soil IN CHINA USING NEAR-INFRARED SPECTROSCOPY

DETECTING MOISTURE CONTENT OF SOUTHERN SOIL IN CHINA USING N...

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亚洲精细农业与计算机农业应用联合大会

作者： Feng Yaoze 1 Li Xiaoyu 1 Li Peiwu 2.Lei Tingwu 3 Wang Wei 1 Xiao Wu 1 Zhou Zhu 1 1. College of Engineering and Technology Huazhong Agricultural University Wuhan China 2. oil crops Research {2. Chinese Academy of Agricultural Sciences Wuhan China 3. {2. of Soil and Water Conservation Chinese Academy of Sciences and Ministry of Water Resources Yangling China

Three types of Southern soil （yellow-brown soil, paddy soil and alluvial soil） were studied based on NIR spectra. Soil moisture analysis model of pretreated soil samples was established by PLS, with R 2.=0.9946 and 0.9919 and RMSECV=0.801%, RMSEP=0.912. for validation and prediction, respectively. 12.unpretreated soil samples were predicted by re-established PLS model based on Kennard-Stone algorithm,with average absolute error reducing from 0.78 % to 0.42.%, which showed that the soil sample space expansion method can improve model prediction accuracy.

关键词： NIR soil water content PCA PLS Kennard-Stone algorithm

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Screening for bacteria degrading oxalic acid and potential for biocontrol of sclerotinia diseases

Screening for bacteria degrading oxalic acid and potential f...

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植物保护2.世纪展望暨第三届全国青年植物保护科技工作者学术研讨会

作者： SY LIU~1 JK ZHANG~1 HM WANG~2.ZY XU~1 (1 oil crops Research {2.,Chinese Academy of Agricultural Science Wuhan 430062.2.China Agricultural University Beijing 100094)

Sclerotinia sclerotiorum(Lib) de Bary,a pathogen which can infect more than 400 plant species and cause serious losses of crop yield,produces a toxin——oxalic acid(OA) in pathogenesis and pure *** studies indicated that OA plays a important role in sclerotinia *** objectives of the studies are to screen microbes which utilize effectively OA and further clone its gene(s) for improvement of both plants for resistance and biocontrol *** detoxification of pathogenic toxin by plants or microbes is a new strategy for disease control.

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Analysis of Genetic Diversity and Phylogenetic Relationship of Ricinus communis Germplasm by SRAP Markers

Analysis of Genetic Diversity and Phylogenetic Relationship ...

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2.09整合植物生物学研讨会

作者： Lu Zheng~1,Jianmin Qi~(2.),Pingping Fang~2.Jianguang Su~3,Jiantang Xu~2.and Aifen Tao~2.1 Fujian Institue of TCM,Fuzhou 350003,China 2.College of crops Sciences/Life Sciences,Fujian Agriculture and Forestry University, Fuzhou 350002.China 3 institute of Bast Fiber crops,Chinese Academy of Agricultural Sciences,Changsha 410006,China.

Castor(Ricinus communis L.)is an important oil *** 2. SRAP primers,2.3 bands were amplified in 81 *** varieties;among them 2.4 bands(81.37%)were *** genetic similarity coefficients of 81 materials ranged from 0.32.58 to

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