检索结果-南通市图书馆

EMMA:An Efficient Massive Mapping Algorithm Using Improved Approximate Mapping Filtering

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2006年第12期38卷 857-864页

作者： Xin ZHANG~1 Zhi-Wei CAO~2.Zhi-Xin LIN~3 Qing-Kang WANG~1* Yi-Xue LI~4 1 Institute of Micro/Nano Science and Technology,shanghai Jiaotong {2.,shanghai 2.0030,china 2.shanghai Center for Bioinformation Technology,shanghai 2.02.5,china 3 College of Life Science and Technology,shanghai Jiaotong {2.,shanghai 2.0030,china 4 Bioinformation Center of shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 2.0031,china Institute of Micro/Nano Science and Technology Shanghai Jiaotong UniversityShanghai 200030 China shanghai Center for Bioinformation Technology Shanghai 200235 China College of Life Science and Technology Shanghai Jiaotong UniversityShanghai 200030 China Bioinformation Center of shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China

Efficient massive mapping algorithm (EMMA),an algorithm on efficiently mapping massivecDNAs onto genomic sequences,has recently been *** process of mapping massive cDNAsonto genomic sequences has been improved using more approximate mapping filtering based on an enhancedsuffix array coupled with a pruned fast hash table,algorithms of block alignment extensions,and *** compared with the classical BLAT software in this field,the computing of EMMA ranges fromtwo to forty-one times faster under similar prediction precisions.

关键词： cDNA mapping maximal exact match enhanced suffix array pruned fast hash table extension algorithm

Identification and Characterization of hmr19 Gene Encoding a Multidrug Resistance Efflux Protein from Streptomyces hygroscopicus *** Strain 10-2.

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第8期36卷 519-528页

作者： Lei QIN , Heng-An WANG1 , Zhong-Qin WU, Xiao-Feng ZHANG, Mei-Lei JIN,Zi-Xin DENG2. and Guo-Ping ZHAOshanghaiResearch Center of Biotechnology, shanghai Institute for Biological Sciences, Chinese Academy of Sciences,Graduate School of the Chinese Academy of Sciences, shanghai 2.02.3, china 1School of Agriculture and Biology, shanghai Jiaotong university, shanghai 2.1101, china 2.chool of Life Science and Biotechnology, shanghai Jiaotong university, shanghai 2.0030, china shanghai Research Center of Biotechnology Shanghai Institute for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200233 China School of Agriculture and Biology Shanghai Jiaotong UniversityShanghai 201101 China School of Life Science and Biotechnology Shanghai Jiaotong UniversityShanghai 200030 China

The hmr19 gene was cloned from Streptomyces hygroscopicus subsp. yingchengensis strain 10- 2., a bacterium strain producing agricultural antibiotics. Sequence similarity comparison indicates that hmr19 gene may encode a predicted protein with 14 putative transmembrane α-helical spanners, belonging to the drug:H+ antiporter-2.family of the major facilitator superfamily. The expression of hmr19 in the mycelium of strain 10-2. was detected by Western blotting analysis. Gene replacement technology was employed to construct an hmr19 disruption mutant. The growth inhibition test against different antibiotics indicated that the mutant strain was 5–2. fold more susceptible to tetracycline, vancomycin and mitomycin C than the parental wild type strain. The mutant took up tetracycline much faster and accumulated more antibiotics than the wild type strain 10-2.. While with the addition of an energy uncoupler, carbonyl cyanide m- chlorophenylhydrazone, the characteristics of the accumulation of [3H]tetracycline in these two strains were almost the same. It was thus concluded that hmr19 encoded a multidrug resistance efflux protein.

关键词： multidrug resistance (MDR) gene replacement (knockout) Streptomyces Streptomyces hygroscopicus subsp. yingchengensis strain 10-2.

Establishment of a Screening System for Selection of siRNA Target Sites Directed against Hepatitis B Virus Surface Gene

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2005年第5期37卷 310-316页

作者： Xiu-Min ZHOU~(1.3) Ju-Sheng LIN~1 Yi SHI~3 De-An TIAN~2.Huan-Jun HUANG~2.He-Jun ZHOU~1 You-Xin JIN~3 1 Institute of Liver Diseases. {2. Hospital, {2. Medical College, Huazhong {2. of Science and Technology, Wuhan 430030, china: 2.Department of Gastroenterology, {2. Hospital, {2. Medical College, Huazhong {2. of Science and Technology, Wuhan 430030, china 3 State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, shanghai 2.0031, china Institute of Liver Diseases Tongji Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030 China State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China Department of Gastroenterology Tongji Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030 China

To study the inhibitory effects of plasmid-derived small interfering RNA(siRNA)andsynthetic siRNA on the expression of the hepatitis B virus surface(HBs)gene,three plasmid-derived siRNAsand one synthetic siRNA that complement the coding region of the HBs gene were *** HBsexpression plasmid pHBs-EGFP was also *** cells were co-transfected with pHBs-EGFPand the above *** HBs mRNA quantities were measured by reverse-transcription PCR,and thelevel of HBs-EGFP fusion protein was quantified by fluorescent *** concentrations of thehepatitis B virus surface antigen(HBsAg)derived from the culture supernatant of transfected HepG2.2.15cells were measured by an enzyme-linked immunosorbent assay(ELISA)*** results showed that thethree plasmid-derived siRNAs and the synthetic siRNA can effectively reduce the quantities of HBs mRNAand *** plasmid-derived siRNA psiRNA1 was found to be the most effective inhibitor of *** can inhibit HBs-EGFP expression by 63.3% and suppress HBs mRNA by 75.6%.To furthersubstantiate the above observations,psiRNA1 was transfected into HepG2.2.15 cells(an HBV secreting cellline).The transfections resulted in almost complete blockage of HBsAg production,whereas control vector-transfected cells secreted high levels of HBsAg 7 days *** conclusion,our data suggeststhat RNA interference(RNAi)is an efficient approach for reducing the level of HBs transcripts and proteinsand for suppressing HBsAg production.

关键词： RNA interference(RNAi) small interference RNA(siRNA) hepatitis B virus surface gene(HBs gene)

Strong law of large numbers for Markov chains indexed by an infinite tree with uniformly bounded degree

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science china Mathematics 2008年第2期51卷 195-202页

作者： HUANG HuiLin~1 YANG WeiGuo~(2.) 1 Department of Mathematics,shanghai Jiaotong {2.,shanghai 2.02.0,china 2.Faculty of Science,Jiangsu {2.,Zhenjiang 2.2.13,china Department of Mathematics Shanghai Jiaotong University Shanghai China Faculty of Science Jiangsu University Zhenjiang China

In this paper,we study the strong law of large numbers and Shannon-McMillan (S-M) theorem for Markov chains indexed by an infinite tree with uniformly bounded *** results generalize the analogous results on a homogene... 详细信息

关键词： Markov chains Shannon-McMillan theorem strong law of large numbers uniformly bounded tree 60F15 60J10

Density-functional formula for strongly correlated systems

在线全文

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2005年第5期15卷 395-401页

作者： WANG Huaiyu1*, HAN Rushan2.and CHEN Nanxian1(1. Department of Physics, Tsinghua university, Beijing 100084, {2. 2. Department of Physics, Peking university, Beijing 100871, {2.) Dept. of Phys. Tsinghua Univ. Beijing 100084 China Dept. of Phys. Peking Univ. Beijing 100871 China

Density functional method is applied for strongly correlated systems. Based on the assumption that the systems are composed of electrons in singly-occupied orbitals and those in doubly-occupied orbitals, a set of self-consistent equations are obtained by standard variation procedure. The equations consist of two parts. One part is to solve the wave functions of the electrons in singly-occupied orbitals and the other is to solve the wave functions of the electrons in doubly-occupied orbitals. The physical meanings of the terms appearing in the equations are discussed.

关键词： density-functional theory strongly correlated systems doubly-occupied orbital two-particle wave function self-consistent equations.

Research and application of flood damage evaluation information system

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

中国有色金属学会会刊：英文版 2005年第S1期15卷 204-207页

作者： SHI Jin-feng~1, WU Zhi-zhou~2. SONG Wei-dong~1 (1. Liaoning Technical university, Fuxin 12.000, china 2.{2., shanghai 2.0092. china) Liaoning Tech Univ Fuxing 123000 Peoples R China tongji Univ Shanghai 200092 Peoples R China

The fast evaluation method of flood damages was explored with the combination of high technologies, such as remote sensing (RS) and geographic information system (GIS), namely the land classification and extraction of flood areas were accomplished in RS image processing software, ENVI, the flood damages information system, digital terrain model and flood damages evaluation information system were developed on GIS platform GeoMedia. The problem of the combination of multi-source data was addressed, furthermore, the problems of how to build the flood damages evaluation model and the inundating elevation model were deeply probed, the calculating formulas were also given. Case study result shows that the evaluation models are correct and data are reliable, we can use it to evaluate real-time flood damages and provide evidences for decision-making of leaders, moreover, it is of great instructive significance to the research of flood damages evaluation theories.

关键词： flood damages GIS remote sensing digital terrain model evaluation

Different-Periodic Travelling Wave Solutions for Nonlinear Equations

在线全文

学校读者我要写书评

暂无评论

Communications in Theoretical Physics 2004年第4期41卷 481-486页

作者： YE Li-Jun~1 LIN Ji~(1,2.1 Department of Physics,Zhejiang Normal university,Jinhua 32.004,china2.Physics Department,{2. Jiao Tong university,{2. 2.0030,china Department of Physics Zhejiang Normal University Jinhua 321004 China Physics Department Shanghai Jiao Tong University Shanghai 200030 China

Using Jacobi elliptic function linear superposition approach for the(1+1)-dimensional Caudrey-Dodd-Gibbon-Sawada-Kotera(CDGSK)equation and the(2.1)-dimensional Nizhnik-Novikov-Veselov(NNV)equation,many new periodic travelling wave solutions with different periods and velocities are obtained based on the knownperiodic *** procedure is crucially dependent on a sequence of cyclic identities involving Jacobi ellipticfunctions sn(ξ,m),cn(ξ,m),and dn(ξ,m).

关键词： linear superposition nonlinear equation travelling wave solution

Diatomaceous silica filter aid filtration for the effective separation of colloidal Cr（OH）＿3 precipitate from tanning wastewater

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 1995年第2期7卷 151-156页

作者： Zhao Youcai Xi Diming Chen Shaowei(School of Environrnental Engineering , tongji university, shanghai {{2., china)

ＤｉａｔｏｍａｃｅｏｕｓｓｉｌｉｃａｆｉｌｔｅｒａｉｄｆｉｌｔｒａｔｉｏｎｆｏｒｔｈｅｅｆｆｅｃｔｉｖｅｓｅｐａｒａｔｉｏｎｏｆｃｏｌｌｏｉｄａｌＣｒ（ＯＨ）＿３ｐｒｅｃｉｐｉｔａｔｅｆｒｏｍｔａｎｎｉｎｇｗａｓｔｅ... 详细信息

ＤｉａｔｏｍａｃｅｏｕｓｓｉｌｉｃａｆｉｌｔｅｒａｉｄｆｉｌｔｒａｔｉｏｎｆｏｒｔｈｅｅｆｆｅｃｔｉｖｅｓｅｐａｒａｔｉｏｎｏｆｃｏｌｌｏｉｄａｌＣｒ（ＯＨ）＿３ｐｒｅｃｉｐｉｔａｔｅｆｒｏｍｔａｎｎｉｎｇｗａｓｔｅｗａｔｅｒＺｈａｏＹｏｕｃａｉ；Ｘ...

关键词： diatornaceous silica filter acid filtration tanning wastewater colloidal Cr(OH)_3 precipitate.

CO observations of massive star forming regions at different evolutionary phases

在线全文

学校读者我要写书评

暂无评论

Science china(Physics,Mechanics & Astronomy) 2006年第5期49卷 626-640页

作者： CHEN Xi1,2. XU Ye1,3, SHEN Zhiqiang1,3 & LI Jingjing1,2.1. {2. Astronomical Observatory, Chinese Academy of Sciences, {2. 2.0030, {2. 2. Graduate School of the Chinese Academy of Sciences, Beijing 100034, {2. 3. Joint Institute for Galaxy and Cosmology, {2. Astronomical Observatory and the university of Science and Technology of {2., {2. 2.0030, {2. shanghai Astronomical Observatory Chinese Academy of Sciences Shanghai China Graduate School of the Chinese Academy of Sciences Beijing China Joint Institute for Galaxy and Cosmology Shanghai Astronomical Observatory and the University of Science and Technology of China Shanghai China

The 12.O (J=1-0), 13CO (J=1-0) and C18O (J=1-0) emissions in 9 massive star forming regions, which are believed to be at different stages of massive star formation, were mapped with the 13.7 m millimeter wave telescope at Qinghai Station of Purple Mountain Observatory. Of the observed 9 sources, 13CO cores were detected in seven of them, and C18O cores in five of them. And only two sources associated with C18O cores and H2. masers showed the extended structures and strong outflows. This is the first detection of outflow associated with IRAS 2.566+582. in the observing field of S152.S153. The physical parameters of cores and outflows for these sources, derived from Local Thermal Equilibrium (LTE) analysis, are presented. These observing results suggest that the C18O cores will only appear when the gas density is high enough and the probability to have an outflow is very high when the clumps show the C18O and H2. maser simulta- neously.

关键词： star formation cloud core outflow H2. maser