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Influence of mild hypothermia on vascular endothelial growth factor and infarct volume in brain tissues after cerebral ischemia in rats

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Neural Regeneration Research 2006年第9期1卷 847-849页

作者： Fei Ye1. Gangming Xi1. Biyong Qin1. Shifeng Wang1. Chengyan Li2 1.epartment of Neurology, People’s Hospital of Yunyang Medical College, Shiyan 442000, Hubei Province, China 2Department of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China Department of Neurology People's Hospital Yunyang Medical College Shiyan 442000 Hubei Province China Department of Neurology Renmin Hospital Wuhan University Wuhan 430060 Hubei Province China

BACKGROUND: It has been demonstrated that mild hypothermia has obvious protective effect on both whole and local cerebral ischemia. However, the definite mechanism is still unclear for the brain protection of mild hypothermia on cerebral edema, inhibiting inflammatory reaction, stabilizing blood brain barrier, etc. OBJECTIVE: To investigate the effect of mild hypothermia on the expression of vascular endothelial growth factor and the infarct volume after cerebral ischemia in rats, and analyze the brain protective mechanism of mild hypothermia. DESIGN: A randomized grouping and controlled animal trial. SETTING: Department of Neurology, People’s Hospital of Yunyang Medical College. MATERIALS: Twenty adult male SD rats of clean degree, weighing (250±30) g, were provided by the animal experimental center, School of Medicine, Wuhan University. The kits for SP immunohistochemistry were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. METHODS: The experiments were carried out in the laboratory of Department of Neurology, Renmen Hospital of Wuhan University from May to July 2005. ① The 20 rats were divided randomly into normal temperature group (n =1.) and mild hypothermia group (n =1.). Models of permanent middle cerebral artery occlusion were established with modified nylon suture embolization. The rats were assessed with the Longa standards: 0 point for without nerve dysfunction; 1.for mild neurological deficit (fore claws could no extend completely); 2 for moderate neurological deficit (circling towards the affected side); 3 for severe neurological deficit (tilting towards the affected side); 4 for coma and unconscious; 1.3 points represented that models were successfully established. The rats of the normal temperature group were fed at room temperature, and those in the mild hypothermia group were induced by hypothermia from 2 hours postoperatively, and the rectal temperature was kept at 34-35 ℃ for 72 hours. ② Measurement of infarct volume: All t

关键词： infarct neurological purchased hemisphere protective minutes Biotechnology stained occlusion Wuhan

A Dialogue Concerning Academic Dishonesty,Academic Standardization and Academic Management

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学术界 2010年第7期 245-249页

作者： Yu Sanding1.Yuan Yuli2 ( 1.Yunmeng Journal,Hunan Province 41.006 2.Academics,Anhui Province 230051.

The recent years have seen many cases of academic dishonesty and corruption in China,which can be explained by the so-called syndrome of academic over-assessment. However,academic standardization remains a secondary factor leading to academic dishonesty. People turn a blind eye to academic dishonesty,which indicates that on one hand,academic dishonesty has become a very common and very serious phenomenon,and on the other hand,people have no way to deal with it. Academic dishonesty can be attributed to the society,the management and scholars themselves. Academic dishonesty can be treated only by the concerted efforts of the society,management and scholars.

关键词： Academic dishonesty Syndrome of academic over-assessment Academic standardization Academic management Academic ecology

Sinorhizobium meliloti nifA mutant induces different gene expression profile from wild type in Alfalfa nodules

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Cell Research 2006年第10期16卷 818-829页

作者： Zi Ying Gong~1.Zhi Shui He~1.Jia Bi Zhu~1.Guan Qiao Yu~1.Hua Song Zou~1.~1.ational Laboratory of Plant Molecular Genetics,Shanghai Institute of Plant Physiology and Ecology,Shanghai Institutes.for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China NationalLaboratoryofPlantMolecularGenetics ShanghaiInstituteofPlantPhysiologyandEcologyShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200032China

Several studies have demonstrated that the Rhizobium nifA gene is an activator of nitrogen fixation acting in *** understand the effects of the Sinorhizobium meliloti nifA gene on Alfalfa,the cDNA-AFLP technique wasemployed to study the changes in gene expression in nifA mutant *** the approximately 3,000 transcript-derived fragments,37 had differential expression *** expression levels were subsequently confirmed by reverseNorthern blot and RT-polymerase chain *** analyses revealed that 21.cDNA fragments corresponded togenes involved in signal communication,protein degradation,nutrient metabolism,cell growth and development.

关键词： cDNA-AFLP gene expression nodule Sinorhizobium meliloti nifA

Adsorption Breakthrough of Benzene in the Fixed Bed of Modified Activated Carbon under Different Humidity Conditions

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Journal of Wuhan University of Technology(Materials Science) 2010年第3期25卷 499-503页

作者：李晶 College of Chemistry and Chemical Engineering Zhongkai University of Agriculture and Engineering

The breakthrough curves of benzene and water on modified activated carbons（ACs） were ***-programmed desorption（TPD） experiments were conducted to measure the TPD curves of benzene and water on modified and unmodified ACs and to estimate the activation energy for the desorption of benzene on the modified *** with unmodified ACs,two modified ACs were prepared by using two different types of silane,designated by KH560 and *** results showed that the activation energy for the desorption of benzene on KH560/AC and 1.06/AC was higher than that on unmodified *** addition,the activation energy for the desorption of water on KH560/AC and 1.06/AC was lower than that on unmodified *** breakthrough curves of benzene obtained from the experimental observations under different humidity conditions were compared with the results of the TPD *** results show that the modified ACs are less affected by water,whereas the unmodified ACs are more affected by water,indicating that surface modification by organosilane compounds can improve the adsorption of benzene on the activated carbo,which weakens the adsorption of water.

关键词： activated carbon benzene water TPD activation energy of desorption breakthrough curves

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航天器电磁兼容预测系统设计与实现

系统仿真学报 2008年第18期20卷 4859-4861,4877页

作者：覃宇建吴超何建国李颖国防科技大学电子科学与工程学院长沙410073 西南电子电信技术研究所成都610041

为使航天器上电子设备互不干扰地工作,必须保证整个系统的电磁兼容性。介绍了航天器电磁兼容预测系统的设计思路,分析了系统的体系结构,着重阐述了各组成模块的工作机理和电磁干扰及电磁兼容预测的计算流程。最后通过一个实例分析表明... 详细信息

为使航天器上电子设备互不干扰地工作,必须保证整个系统的电磁兼容性。介绍了航天器电磁兼容预测系统的设计思路,分析了系统的体系结构,着重阐述了各组成模块的工作机理和电磁干扰及电磁兼容预测的计算流程。最后通过一个实例分析表明该系统能够完成对航天器进行电磁兼容预测的任务。

关键词：电磁兼容电磁干扰电磁兼容预测航天器

Telmisartan induces melanoma cell apoptosis and synergizes with vemurafenib in vitro by altering cell bioenergetics

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Cancer Biology & Medicine 2019年第2期16卷 247-263页

作者： Jelena Grahovac Tatjana Srdic-Rajic Juan Francisco Santibanez Marijana Pavlovic Milena Cavic Sinisa Radulovic1 Laboratory for Experimental Pharmacology Institute for Oncology and Radiology of Serbia Belgrade 11000 Serbia Department of Molecular Oncology Institute for Medical Research University of Belgrade Belgrade 11000 Serbia Integrative Center for Biology and Applied Chemistry (CIBQA) Bernardo O'Higgins University Santiago 8370854 Chile

Objective: Despite recent advancements in targeted therapy and immunotherapies, prognosis for metastatic melanoma patients remains extremely poor. Development of resistance to previously effective treatments presents a serious challenge and new approaches for melanoma treatment are urgently needed. The objective of this study was to examine the effects of telmisartan, an AGTR1.inhibitor and a partial agonist of PPARγ, on melanoma cells as a potential agent for repurposing in melanoma ***: Expression of AGTR1.and PPARγ m RNA in melanoma patient tumor samples was examined in publicly available datasets and confirmed in melanoma cell lines by qRT-PCR. A panel of melanoma cell lines was tested in viability, apoptosis and metabolic assays in presence of telmisartan by flow cytometry and immunocytochemistry. A cytotoxic effect of combinations of telmisartan and targeted therapy vemurafenib was examined using the Chou-Talalay combination index ***: Both AGTR1.and PPARγ mRNA were expressed in melanoma patient tumor samples and decreased compared to the expression in the healthy skin. In vitro, we found that telmisartan decreased melanoma cell viability by inducing cell *** glucose uptake, but not utilization, in the presence of telmisartan caused the fission of mitochondria and release of reactive oxygen species. Telmisartan altered the cell bioenergetics, thereby synergizing with vemurafenib in vitro, and even sensitized vemurafenib-resistant cells to the ***: Given that the effective doses of telmisartan examined in our study can be administered to patients and that telmisartan is a widely used and safe antihypertensive drug, our findings provide the scientific rationale for testing its efficacy in treatment of melanoma progression.

关键词： Melanoma telmisartan apoptosis mitochondria reactive oxygen species targeted therapy

Experimental and theoretical study of^(1.)C shielding tensors in new-style molybdenum complex

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Science China Chemistry 2004年第3期47卷 214-221页

作者： HOU Guangjin1.2, WANG Liying2, LU Xiaoming3, ZHENG Anmin2, DENG Feng2 &YE Chaohui1.2 1. Department of Physics, Huazhong University of Science and Technology, Wuhan 430074, China 2. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chi-nese Academy of Sciences, Wuhan 430071. China 3. Department of Chemistry, Capital Normal University, Beijing 1.0037, China 1. Department of Physics Huazhong University of Science and Technology 430074 Wuhan China 2. Chinese Academy of Sciences State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Wuhan Institute of Physics and Mathematics 430071 Wuhan China 3. Department of Chemistry Capital Normal University 100037 Beijing China

1.C 2D-PASS spectra of two new cis-dioxo catecholatomolybdenum complexes (NH2CH2NH2CHCH2)2(H+)3[MovO 2(C6H4O2)2] and (NH2CH2CH2CH2NH2)2(H+)3[Mo(v)O2 (C2H2O2)2] have been obtained by solid-state nuclear magnetic resonance (NMR), in which the spinning sidebands were well-separated. The principal components of the 1.C shielding tensors were extracted by theoretically fitting the intensities of 1.C spinning sidebands. The effects of counter cations on 1.C chemical shift isotropy and shielding tensor of cis-dioxo catecholatomolybdenum complex anion [Mo (v)O2(C6H4O2)2]3? were studied, comparing the 1.C CSA of those carbon sites in complex anions with that of the counter cations. Based on the known structure of the molybdenum complex crystal, theoretical values of 1.C shielding tensors were calculated by the ainitio GIAO method, in comparison with the experimental results.

关键词： NMR, magic angle spinning, chemical shift anisotropy, shielding tensor, cis-dioxo-monomolybdenum.

Composite Cathode Bi_(1.1.)Sr_(0.43)O_(2.1.)-Ag for Intermediate-temperature Solid Oxide Fuel Cells

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Journal of Wuhan University of Technology(Materials Science) 2008年第3期23卷 350-353页

作者：高展张萍 Faculty of Material Science and Chemical Engineering China University of Geosciences

Composites consisting of strontium stabilized bismuth oxide （Bi1.1.Sr0.43O2.1., SSB） and silver were investigated as cathodes for intermediate-temperature solid oxide fuel cells with doped ceria electrolyte. There were no chemical reactions between the two components. The microstructure of the interfaces between composite cathodes and Ce0.8Sm0.2O1.9 （SDC） electrolytes was examined by scanning electron microscopy （SEM）. Impedance spectroscopy measurements show that the performance of cathode fired at 700 ℃ is the best. When the content of Ag2O is 70 wt%, polarization resistance values for the SSB-Ag cathodes are as low as 0.2 Ωcm^2 at 700℃ and 0.29 Ωcm^2 at 650℃. These results are much smaller than some of other reported composite cathodes on doped ceria electrolyte and indicate that SSB-Ag composite is a potential cathode material for intermediate temperature SOFCs.

关键词： solid oxide fuel cells (SOFCs) composite cathode strontium stabilized bismuth oxide (SSB) samaria doped ceria (SDC)

The Effect of TanshinoneⅡ A upon the TGF-beta1.Smads Signaling Pathway in Hypertrophic Myocardium of Hypertensive Rats

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2009年第4期29卷 476-480页

作者：李永胜杨宇平于丹梁黔生 Department of Emergency Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Pharmacology School of PharmacyXinxiang Medical University

To investigate the molecular mechanism by which Tanshinone Ⅱ A （TSN Ⅱ A） prevents left ventricular hypertrophy （LVH）, we examined the expression of AT1., TGF-β1.and Smads gene in the hypertrophic myocardium of hypertensive rats with abdominal aorta constriction. LVH model was established by creating abdominal aorta constriction. Four weeks later, animals were randomly divided into 4 groups with 8 animals in each. One group was used as model control, the other three groups were treated with TSN ⅡA （20 mg/kg）, TSN ⅡA （1. mg/kg） and valsartan （1. mg/kg）, respectively. Another 8 SD rats were subjected to sham surgery and served as blank control. After 8- week treatment, the caudal artery pressure of the animals was measured. The tissues of left ventricle were taken for the measurement of the left ventricular mass index （LVMI） and pathological sectioning and HE-staining were used for determining the myocardial fiber dimension （MFD）. The mRNA expression of AT1., protein expression of TGF-betal and activity of Smad-2, 4, 7 were detected by RT-PCR and Western blotting, respectively. Our results showed that （1. the blood pressure of rats treated with TSN Ⅱ A, either at high or low dose, was significantly higher than those in the control and valsartan-treated group （P〈0.01. P〈0.05）; （2） LVMI and MFD in TSN Ⅱ A and valsartan-treated rats were higher than those in the control group （P〈0.05） but significantly lower than those in the model control （P〈0.01.; （3） the high doses of TSN Ⅱ A and valsartan significantly down-regulated the mRNA expression of AT 1. and protein expression of TGF-beta l and Smad-3 in the hypertrophic myocardium （P〈0.01., and TGF-betal in valsartan-treated animals was more significantly lower than that in rats treated with TSN Ⅱ A; （4） the two doses of TSN Ⅱ A and valsartan significantly up-regulated the protein expression of Smad-7 in the hypertrophic myocardium （P〈0.01., and Smad-7 in the animals treated with high-dose TSN Ⅱ A was significantly higher tha

关键词： Tanshinone ⅡA pressure overloading myocardial hypertrophy AT1. TGF-betal Smads