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Generation of large fifth-order nonlinear phase shifts by use of lossless χ^((2)) cascaded nonlinear processes

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Science china(Physics,Mechanics & Astronomy) 2004年第1期47卷 1-7页

作者： XU Guang1. QIAN Liejia2, WANG Tao2, FAN Dianyuan1.2 & LI Fuming2 1. shanghai {1. of Optics and Fine Mechanics, chinese academy of sciences, shanghai 201.00, china 2. State Key Laboratory for Advanced Photonic Materials and Devices, Fudan University, shanghai 200433, china Correspondence should be addressed to Xu Guang (email: xuguany@***) Received March 9, 2003 1. shanghai {1. of Optics and Fine Mechanics Chinese Academy of Sciences 201800 Shanghai China 2. State Key Laboratory for Advanced Photonic Materials and Devices Fudan University 200433 Shanghai China

It is shown that the cascaded fifth-order nonlinear phase shifts will increase with energy loss in the cascaded processes. Essentially different from the multi-photon absorption accompanied with inherent material nonlinearities, the loss of fundamental wave in a cascaded process is controllable and suppressible. By introducing difference frequencies generated from the reaction between the fundamental and its second harmonic after the cascaded processes, the fundamental wave can be free of energy loss, while the large cascaded fifth-order nonlinear phase shift is maintained.

关键词： cascaded nonlinear phase shifts, phase mismatching, harmonic conversions.

A Novel Single-beam Magneto-optical Atom Trap

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chinese Journal of Lasers 2000年第3期9卷 64-71页

作者： GAO Wei-1. YIN Jianping- 1.2 WANG Yuzhu-1. ( -1.Laboratory for Quantum Optics, shanghai {1. of Optics and Fine Mechanics, The chinese academy of sciences, shanghai 201.00, china -2 Department of Physics, Suzhou University, Suzhou 21.006, Ch 中科院上海光学精密机械所量子光学开放实验室上海

A novel single-Gaussian-beam magneto-optical trap with a lens and spherical mirror is proposed. Our analysis and calculations show that it can trap as many as 1.-6 atoms with a temperature of ～330 μK. Because of its extraordinary simplicity and much efficaciousness, the proposed single-beam trapping scheme will have many applications in the experiments of atom optics with cold and dense atoms.

关键词： laser cooling and trapping single-beam magneto-optical trap Gaussian laser beam

Optimum segmentation of simple objects in high-resolution remote sensing imagery in coastal areas

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Science china Earth {1. 2006年第11期49卷 1195-1203页

作者： CHEN Jianyu1.2, PAN Delu1.& MAO Zhihua1.1. State Key Laboratory of Satellite Ocean Environment Dynamics, Second institute of Oceanography, State Oceanic Admini- stration, Hangzhou 31.01.,{1. 2. shanghai institute of Technical Physics, chinese academy of Science, shanghai 2.0083,{1. 1. State Key Laboratory of Satellite Ocean Environment Dynamics Second Institute of Oceanography State Oceanic Administration Hangzhou 310012 China 2. shanghai {1. of Technical Physics Chinese Academy of Science Shanghai 200083 China

The optimum segmentation of ground objects in a landscape is essential for interpretation of high-resolution remotely sensed imagery and detection of objects; and it is also a technical foundation to efficiently use spatial information in remote sensing imagery. Landscapes are complex system composed of a large number of heterogeneous components. There are many explicit homogeneous image objects that have similar spectral character and yet differ from surrounding objects in high-resolution remote sensing imagery. Thereby, a new concept of Distinctive Feature of fractal is put forward and used in deriving Distinctive Feature curve of fractal evolution in multiscale segmentation. Through distinguishing the extremum condition of Distinctive Feature curve and the inclusion relationship of fractals in multiscale representation the Scalar Order is built. This can help to determinate the optimum scale in image segmentation for simple-objects, and the potential meaningful image-object fitting the intrinsic scale of the dominant landscape object can be obtained. Based on the application in high-resolution remote sensing imagery in coastal areas, a satisfactory result was acquired.

关键词： optimum scale, multiscale segmentation, image interpretation, remote sensing, coastal area.

Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer

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Cell Research 2001年第2期11卷 149-155页

作者： HUANG JIAN QIU, ZHI MING WEI, HAI LONG AN, YU XIAN ZHU National Laboratory of plant Molecular Genetics, institute of plant physiology and Ecology, shanghai institutes for Biological sciences, chinese academy of sciences, shanghai 2.0032, china National La National Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Chinese Academy of Sciences Shanghai. jqhuang@***

Immature embryos of rice varieties 'Xiushui1.' and 'Chunjiang 1.' precultured for 4d were infected and transformed by Agrobacterium tumefaciens strain EHA1.1.pExT7 (containing the spider insecticidal gene). The resistant cant were transferred onto the differentiation medium and plants were regenerated. The transformation frequency reached 56%-72% measured as numbers of Geneticin (G41.)-resistant calli produced and 36%-60% measured as numbers of transgenic plants regenerated, respectively. PCR and Southern blot analysis of transgenic plants confirmed that the T-DNA had been integrated into the rice genome. Insect bioassays using T1.transgenic plants indicated that the mortality of the leaffolder (Cnaphalocrasis medinalis) after 7d of leaf feeding reached 38%-61. and the corrected mortality of the striped stem borer (Chilo suppressalis) after 7d of leaf feeding reached 1.%-75%. The insect bioassay results demonstrated that the transgenic plants expressing the spider insecticidal protein conferred enhanced resistance to these pests.

关键词： Rice Agrobacterium tumefaciens spider insecticidal gene transgenic plant Leaffolder striped stem borer insect bioassay.

Identification and Characterization of hmr1. Gene Encoding a Multidrug Resistance Efflux Protein from Streptomyces hygroscopicus *** Strain 1.-22

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 519-528页

作者： Lei QIN , Heng-An WANG1 , Zhong-Qin WU, Xiao-Feng ZHANG, Mei-Lei JIN,Zi-Xin DENG2, and Guo-Ping ZHAOshanghaiResearch Center of Biotechnology, shanghai institute for Biological sciences, chinese academy of sciences,Graduate School of the chinese academy of sciences, shanghai 200233, china 1School of Agriculture and Biology, shanghai Jiaotong University, shanghai 201101, china 2School of Life Science and Biotechnology, shanghai Jiaotong University, shanghai 2.0030, china shanghaiResearch Center of Biotechnology Shanghai Institute for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences School of Agriculture and Biology Shanghai Jiaotong University

The hmr1. gene was cloned from Streptomyces hygroscopicus subsp. yingchengensis strain 1.- 22, a bacterium strain producing agricultural antibiotics. Sequence similarity comparison indicates that hmr1. gene may encode a predicted protein with 1. putative transmembrane α-helical spanners, belonging to the drug:H+ antiporter-2 family of the major facilitator superfamily. The expression of hmr1. in the mycelium of strain 1.-22 was detected by Western blotting analysis. Gene replacement technology was employed to construct an hmr1. disruption mutant. The growth inhibition test against different antibiotics indicated that the mutant strain was 5–20 fold more susceptible to tetracycline, vancomycin and mitomycin C than the parental wild type strain. The mutant took up tetracycline much faster and accumulated more antibiotics than the wild type strain 1.-22. While with the addition of an energy uncoupler, carbonyl cyanide m- chlorophenylhydrazone, the characteristics of the accumulation of [3H]tetracycline in these two strains were almost the same. It was thus concluded that hmr1. encoded a multidrug resistance efflux protein.

关键词： multidrug resistance (MDR) gene replacement (knockout) Streptomyces Streptomyces hygroscopicus subsp. yingchengensis strain 1.-22

Detection of protein microarrays by oblique-incidence reflectivity difference technique

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Science china(Physics,Mechanics & Astronomy) 2010年第2期53卷 306-309页

作者： WEN Juan1,LU Heng1,WANG Xu1,YUAN Kun1,L HuiBin1,ZHOU YueLiang1,JIN KuiJuan1,YANG GuoZhen1,LI Wei2 & RUAN KangCheng2 1 Beijing National Laboratory for Condensed Matter Physics,institute of Physics,chinese academy of sciences,Beijing 100190,china 2 Key Laboratory of Proteomics,institute of Biochemistry and Cell Biology,shanghai institute for Biological sciences,chinese academy of sciences,shanghai 2.0031,china 1. Beijing National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing 100190 China2. Key Laboratory of Proteomics Institute of Biochemistry and Cell Biology Shanghai Institute for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Biological microarrays with different proteins and different protein concentrations are detected without external labeling by an oblique-incidence reflectivity difference (OIRD) technique. The initial experiment results reveal that the intensities of OIRD signals can distinguish the different proteins and concentrations of protein. The OIRD technique promises feasible applications to life sciences for label-free and high-throughput detection.

关键词： oblique-incidence reflectivity difference protein microarray label-free detection

Tape Casting of Al_2O_3-TiB_2/Al_2O_3-Nano-TiC Multilayer Composites

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Journal of Materials Science & Technology 1997年第4期13卷 324-326页

作者： Yuping ZENG Dongliang JIANG Shouhong TAN and Jingkun GUO(shanghai {1. of Ceramics, chinese academy of sciences, shanghai 2.0050, china) shanghai {1. of Ceramics Chinese Academy of Sciences Shanghai 200050 China

Ceramic tapes, containing Al2O3-25 wt pct TiB2(B) and Al2O3-25 wt pct nano-TiC (c), have been obtained by tape casting process. Numerous tapes (about 60～80 tapes) were prepared by stacking in turn the composition (B) and (C), laminating under 1. MPa pressure, eliminating the solvent and burning out the polymer additives. The final green bodies were hot pressed at 1.50℃ and 30 MPa. The composite has a bending strength of 568 MPa and a fracture toughness of 5.8 M Pa·m1.2. SEM analysis exhibits that Al2O3 particle growth was inhibited by TiC particles in C. but TiB2 particles could not hinder Al2O3 growth in B. The curves of GTA indicates that all organic additives could be removed completely above 600℃

关键词： Nano TiB Al Tape Casting of Al2O3-TiB2/Al2O3-Nano-TiC Multilayer Composites TiC

Auxin distribution and transport during embryogenesis and seed germi-nation of Arabidopsis

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Cell Research 2001年第4期11卷 273-278页

作者： NI DI AN, LING JIAN WANG, CHUN HONG DING, ZHI HONG XU, institute of {1. physiology and Ecology, shanghai institutes for Biological sciences, chinese academy of sciences, shanghai 2.0032, china College of Bioscience, East china Normal University, Shangha 1.0} of {1. physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China 1.0} of {1. physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China 2College of Bioscience East China Normal University Shanghai 200062 China 3College of Life sciences Peking University Beijing 100871 China

Auxin distribution during embryogenesis and seed germination were studied with transgenic Arabidopsis plants expressing GUS gene driven by a synthetic DR5 promoter,an auxin responsive promoter. The results showed that GUS activity is higher in ends of hypophysis and cotyledon primordia of heart-, torpedo- and cotyledon-stage embryos, leaf tip area, lateral root primordia, root apex and cotyledon of young seedlings. And GUS accumulated in root apex of the seedlings grown on auxin transport inhibitor containing media. All these suggested that above-mentioned part of the organs and tissues have a higher level of auxin, and auxin polar transport inhibitor could cause the accumulation of auxin in root apex. And auxin transport inhibitor also resulted in aberration of Arabidopsis leaf pattern formation, root gravitropism and elongation.

关键词： Auxin auxin distribution auxin polar transport auxin-responsive promoter transgenic Ara- bidopsis.

Overcoming multidrug resistance(MDR) in cancer by nanotechnology

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Science china Chemistry 2010年第11期53卷 2226-2232页

作者： BU HuiHui,GAO Yu & LI YaPing shanghai {1. of Materia Medica,chinese academy of sciences,shanghai 201.03,china 1. shanghai {1. of Materia Medica Chinese Academy of Sciences Shanghai 201203 China

The emerging nanotechnology-based drug delivery holds tremendous potential to deliver chemotherapeutic drugs for treatment of multidrug resistance(MDR) *** drug delivery system could improve the pharmacokinetic behavior of antitumor drugs,deliver chemotherapeutic drugs to target sites,control release of drugs,and reduce the systemic toxicity of drugs in MDR *** review addresses the use of nanotechnology to overcome MDR classified on the bases of the fundamental mechanisms of MDR and various approaches to deliver drugs for treatment of MDR cancer.

关键词： nanotechnology nanoparticles multidrug resistance chemotherapeutic drugs cancer drug delivery system