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Ultra Short Pulsed Laser Deposition Technology for Industrial Applications

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材料科学与工程（中英文b版） 2015年第5期5卷 196-205页

作者： dr Jari Liimatainen Ville Kekkonen Jarkko Piirto Juho Kaisto dr Aleksey Zolotukhin dr Saumyadip Chaudhuri Picodeon Oy Ltd Piisilta 1 91100 li Finland

An optimized ion trap geometry to measure quadrupole shifts of ^(171)Yb~＋ clocks

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Chinese Physics b 2016年第11期25卷 230-237页

作者： N batra b K Sahoo S De Academy of Scientific and Innovative Research CSIR-NPL Campus New Delhi India CSIR-National Physical Laboratory Dr. K. S. Krishnan Marg New Delhi 110012 India Theoretical Physics Division Physical Research Laboratory Navrangpura Ahmedabad 380009 India

We propose a new ion-trap geometry to carry out accurate measurements of the quadrupole shifts in the （171）Yb *** trap will minimize the quadrupole shift due to the harmonic component of the confining potential by an order of *** will be useful to reduce the uncertainties in the clock frequency measurements of the 6s 2S（1/2）→4f（13）6s2 2F（7/2）and 6s 2S（1/2）→5d2D（3/2） transitions,from which we can deduce the precise values of the quadrupole moments（0s） of the 4f（13）6s2 2F（7/2） and 5d2D（3/2） ***,it may be able to affirm the validity of the measured 0 value of the4f（13）6s22F（7/2） state,for which three independent theoretical studies defer almost by one order of magnitude from the *** also calculate 0s using the relativistic coupled-cluster（RCC） *** use these 0 values to estimate the quadrupole shift that can be measured in our proposed ion trap experiment.

关键词： validity minimize carry clock relativistic precise deduce magnitude expectation spatially

NANOSTRUCTURES OF FUNCTIONAL bLOCK COPOLYMERS

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Chinese Journal of Polymer Science 2000年第3期18卷 255-262页

作者： Guojun Liu Department of Chemistry, University of Calgary, 2500 University dr., NW, Calgary, Alberta, Canada T2N IN4 Univ Calgary Dept Chem Calgary AB T2N 1N4 Canada

Nanostructure fabrication from block copolymers in my group normally involves polymer design, synthesis, self-assembly, selective domain crosslinking, and sometimes selective domain removal. Preparation of thin films withnanochannels was used to illustrate the strategy we took. In this particular case, a linear triblock copolymer polyisoprenc-block-poly(2-cinnamoylethyl methacrylate)-block-poly(t-butyl acrylate), PI-b-PCEMA-b-PtbA, was used. Films, 25 to50 μm thick, were prepared from casting on glass slides a toluene solution of PI-b-PCEMA-b-PtbA and PtbA homopolymer,hPtbA, where hPtbA is shorter than the PtbA block. At the hPtbA mass faction of 20% relative to the triblock or the totalPtbA (hPtbA and PtbA block) volume fraction of 0.44, hPtbA and PtbA formed a seemingly continuous phase in the matrixof PCEMA and Pl. Such a block segregation pattern was locked in by photocrosslinking the PCEMA domain. Nanochannelswere formed by extracting out hPtbA with solvent. Alternatively. larger channels were obtained from extracting out hPtbAand hydrolyzing the t-butyl groups of the PtbA block. Such membranes were not liquid permeable but had gas permeabilityconstants ～6 orders of magnitude higher than that of low-density polyethylene films.

关键词： Nanostructures block copolymers Nanochannel generation Self-Assembly Polyisoprene-b-poly(2-cinnanoylethyl methacrylate)-b-poly(t-butyl acrylate)

Classification of Glaucoma in Retinal Images Using Efficientnetb4 Deep Learning Model

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Computer Systems Science & Engineering 2022年第12期43卷 1041-1055页

作者： A.Geetha N.b.Prakash National Engineering College Kovilpatti628502India

Today, many eye diseases jeopardize our everyday lives, such as Diabetic Retinopathy (dr), Age-related Macular Degeneration (AMD), and *** is an incurable and unavoidable eye disease that damages the vision ofoptic nerves and quality of life. Classification of Glaucoma has been an active fieldof research for the past ten years. Several approaches for Glaucoma classification areestablished, beginning with conventional segmentation methods and feature-extraction to deep-learning techniques such as Convolution Neural Networks (CNN). Incontrast, CNN classifies the input images directly using tuned parameters of convolution and pooling layers by extracting features. but, the volume of training datasetsdetermines the performance of the CNN;the model trained with small datasets,overfit issues arise. CNN has therefore developed with transfer learning. The primary aim of this study is to explore the potential of EfficientNet with transfer learning for the classification of Glaucoma. The performance of the current workcompares with other models, namely VGG16, InceptionV3, and Xception usingpublic datasets such as RIM-ONEV2 & V3, ORIGA, drISHTI-GS1, HRF, andACRIMA. The dataset has split into training, validation, and testing with the ratioof 70:15:15. The assessment of the test dataset shows that the pre-trained EfficientNetb4 has achieved the highest performance value compared to other models listedabove. The proposed method achieved 99.38% accuracy and also better results forother metrics, such as sensitivity, specificity, precision, F1_score, Kappa score, andArea Under Curve (AUC) compared to other models.

关键词： Convolution neural network deep learning fundus image glaucoma image classification

Evolutionary Methods in biotechnology: Clever Tricks for Directed Evolution 1

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Learning to become addicted,one synapse at a time

2004年

作者： dr. Susanne brakmann dr. Andreas Schwienhorst

ISBN: (数字)9783527603695

ISBN: (纸本)9783527307999

Miniturization and high throughput assay technology have brought the power of molecular evolution to the bioscience laboratory. Applied wisely, the evolutionary approach can quickly yield the desired result even where other methods have failed. From library generation by random or directed mutagenesis to screening and selection techniques -- the crucial steps for successful evolutionary biotechnology are described in detail in this practical guide that also includes valuable troubleshooting hints on frequently encountered problems. Modern methods for the surface display of peptides and proteins, selective enrichment of nucleic acid aptamers and high-throughput screening of industrial biocatalysts are explained, and computer-based methods for in silico protein and RNA engineering are described as an alternative to in vitro approaches. A special section covers the patenting regulations with regard to biotechnological innovations derived from directed evolution. As an added bonus, a CD-ROM is included that contains software tools for library design, selection of mutagenesis positions, and various predictive algorithms. In short, this practice oriented handbook is an indispensable tool for every scientist working in this interdisciplinary research area.

关键词： Genetic engineering.

来源：畅想之星图书光盘版（南... 评论

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Neural Regeneration Research 2024年第2期19卷 401-402页

作者： Alessio Attardo Sidney b.Cambridge Leibniz Institute for Neurobiology MagdeburgGermany Institute for Anatomy II Dr.Senckenberg AnatomyGoethe-University Frankfurt am MainFrankfurtGermany

Addiction has been extensively studied on many levels:from the molecular level,with various“omics”approaches(Natividad et al.,2018;Grecco et al.,2021),to the clinical level,with psychotherapy and MRI imaging(Ceceli ... 详细信息

关键词： clinical therapy

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类艾滋病病毒单克隆抗体的研究

中国人兽共患病杂志 1992年第1期8卷 9-10页

作者：盛祖恬 dr G V Hoosiel dr M Thouless 浙江省农科院研究所华盛顿大学医学院动物医学系华盛顿大学医学院病理生物学系

本文采用类艾滋病病毒(SAIDV)D型抗原免疫bALb/C小鼠,取其脾细胞与小鼠骨髓瘤细胞SP2/0在40％PEG作用下进行细胞融合,杂交瘤细胞经HAT筛选,用ELISA法检测阳性抗体,经3次克隆和3个月的传代及液氮保存试验,获得一株能分泌抗SAIDV的单克隆... 详细信息

本文采用类艾滋病病毒(SAIDV)D型抗原免疫bALb/C小鼠,取其脾细胞与小鼠骨髓瘤细胞SP2/0在40％PEG作用下进行细胞融合,杂交瘤细胞经HAT筛选,用ELISA法检测阳性抗体,经3次克隆和3个月的传代及液氮保存试验,获得一株能分泌抗SAIDV的单克隆抗体的淋巴细胞杂交瘤,编号为WA-b_1,其腹水滴度为1:1×10^(-6),经蛋白亚类测定为IgG2a,能被特异性抗SAIDV血清所阻断,证明该单克隆抗体具有抗SAID病毒的特异性,为此作为第一个建立抗SAIDV(D)型单克隆抗体杂交瘤细胞株而闻世。

关键词：类艾滋病病毒单克隆抗体克隆

Copper solvent extraction from alkaline cyanide solution with guanidine extractant LIX 7950

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Transactions of Nonferrous Metals Society of China 2010年第6期20卷 1136-1140页

作者： F.XIE D.b.drEISINGER Department of Materials Engineering University of British Columbia

The use of the guanidine extractant LIX 7950 extracting copper and cyanide from alkaline cyanide solution was *** extraction of copper and cyanide under different initial copper and extractant concentrations was examined and the stoichiometric extraction constant of Cu(CN)32- with LIX 7950 was *** the distribution coefficient and the stoichiometric extraction constant of Cu(CN)3 2-with LIX 7950 decrease when the temperature is varied from 25℃to 45℃, indicating the extraction process is *** calculated enthalpy change of the reaction(-HΘ)is about-190 kJ/*** copper extraction isotherms under different molar ratios of cyanide to copper are *** preferential extraction of Cu(CN)32- over Cu(CN)4 3-and CN -has been confirmed and a high cyanide-to-copper molar ratio tends to suppress copper loading. The loaded copper and cyanide can be stripped efficiently by the moderately strong NaOH solutions(0.5-1.0 mol/L)and the presence of NaCN in the stripping solution facilitates copper stripping.

关键词： copper cyanide solvent extraction guanidine

Solid-liquid extraction of Gd(Ⅲ) and separation possibilities of rare earths from phosphoric acid solutions using Tulsion CH-93 and Tulsion CH-90 resins

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Journal of Rare Earths 2012年第12期30卷 1270-1275页

作者： S.Radhika V.Nagaraju b.Nagaphani Kumar M.Lakshmi Kantam b.Ramachandra Reddy Inorganic Chemistry Division Indian Institute of Chemical Technology(IICT) Rubamin Limited Halol-389 350India

Solid-liquid extraction of gadolinium was investigated from phosphoric acid medium using commercial amino phosphonic acid resin, Tulsion CH-93. The experimental conditions studied included equilibration time, acid concentration, mass of the resin, metal concentration, loading and elution. The percent extraction of Gd（Ⅲ） was studied as a function of phosphoric acid （0.05-3 mol/L） using Tulsion CH-93 resin. The corresponding lgD vs. equilibrium pH plot gave straight line with a slope of 1.8. The percent extraction decreased with acid co centration increasing, conforming ion exchange mechanism. Under observed experimental conditions the loading capacity of Tulsion CH-93 for gadolinium was 10.6 mg/g. Among several eluants screened, the quantitative elution of Gd（III） from loaded Tulsion CH-93 was obtained with ammonium oxalate （0.15 mol/L）. The extraction behavior of commonly associated metals with gadolinium was studied as a function of phosphoric acid concentration. Tulsion CH-93 resin showed selective extraction towards heavy rare earths （Lu and Yb） which could be separated from other rare earths at 3 mol/L H3PO4, similar to wet phosphoric acid （3-5 mol/L）. On the other hand Gd（Ⅲ） and other rare earths were studied with chelating resin Tulsion CH-90. Light rare earths were highly extracted and these could be separated from heavy rare earths and Gd.

关键词： gadolinium phosphoric acid Tulsion CH-93 Tulsion CH-90 separation factors rare earths