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聊城师院学报（自然科学版） 2002年第3期15卷 39-40页

作者：许恒龙徐梅聊城大学生物系山东聊城252059

1998年4～6月,对聊城市区主要水域,采用活体采样,研究尾草履虫自然培养中种群增长曲线与水质污染的相关性。结果表明,草履虫培养中种群增长曲线与水域水质的污染程度呈一定的相关性。

关键词：尾草履虫种群增长曲线水质监测水质污染东昌湖老运河水质评价

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东昌湖眼虫属(Euglena)种群的研究

聊城师院学报（自然科学版） 2001年第4期14卷 64-65页

作者：许恒龙徐伟聊城师范学院生物系山东聊城252059

１９９９年４月下旬至６月上旬,采用沉降法采样,对聊城东昌湖三个湖区的眼虫(Ｅｕｇｌｅｎａ)进行了检测,共检出眼虫１１种,发现水体的大小和不同的污染程度都对眼虫的种类组成和生物数量有着显著的影响．

关键词：东昌湖眼虫属水质污染浮游生物形态学种类组成生物数量生物种群

海洋纤毛虫实验生态学研究 IV:不同人工海水培养液及温度对扇形游仆虫种群增长的影响

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应用与环境生物学报 2007年第1期13卷 41-45页

作者：许恒龙周丽沙于丽周晓苏江居国田玉娟苗月丽聊城大学生命科学学院原生动物学研究室山东聊城252059

为了揭示出海洋纤毛虫在人工海水中及不同温度下的种群动力学,利用实验生态学方法,就4种培养液及2种(17℃和23℃)温度对海洋纤毛虫扇形游仆虫(Euplotesvannus Müller,1786)种群增长的影响进行了初步探讨.结果显示①该种纤毛虫原生动物... 详细信息

为了揭示出海洋纤毛虫在人工海水中及不同温度下的种群动力学,利用实验生态学方法,就4种培养液及2种(17℃和23℃)温度对海洋纤毛虫扇形游仆虫(Euplotesvannus Müller,1786)种群增长的影响进行了初步探讨.结果显示①该种纤毛虫原生动物在4种人工海水培养液中的种群增长率大小顺序为牛肉浸膏培养液>米粒培养液>酵母粉培养液>蛋白胨培养液;②种群密度大小顺序依次为米粒培养液>牛肉浸膏培养液>酵母粉培养液>蛋白胨培养液;③指数增长期及稳定期在米粒培养液中均长于牛肉浸膏培养液;④随着培养温度的升高,种群增长率增大,指数增长期及稳定期缩短;⑤温度系数(Q10)的大小顺序为蛋白胨培养液>牛肉浸膏培养液>米粒培养液>酵母粉培养液.结果表明,米粒人工海水培养液不仅适合于保种培养,而且适合于种群的扩大培养,而牛肉浸膏培养液更适合于种群的快速扩大培养,其他两种培养液则只适合于保种培养.

关键词：扇形游仆虫种群增长世代时间温度系数人工海水

以浮游植物和MTSI综合评价网箱养殖草鱼和鲤鱼对鱼塘水质的污染和富营养化的研究

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聊城大学学报（自然科学版） 1997年第1期15卷 77-84页

作者：许恒龙闫华超宋兴民

１９９４～１９９５年在中国科学院水生生物研究所渔业高产开发研究基地之一，山东聊城嘉明有限公司水产养殖场，对网箱养殖草鱼、鲤鱼鱼塘的浮游植物进行了种群组成、数量、优势种、污染指示种、多样性指数、叶绿素含量和ＭＴＳＩ等参... 详细信息

１９９４～１９９５年在中国科学院水生生物研究所渔业高产开发研究基地之一，山东聊城嘉明有限公司水产养殖场，对网箱养殖草鱼、鲤鱼鱼塘的浮游植物进行了种群组成、数量、优势种、污染指示种、多样性指数、叶绿素含量和ＭＴＳＩ等参数的初步研究，并利用后六项参数对鱼塘水质污染程度和富营养化程度进行了评价，浮游植物（包括植鞭毛虫）含量平２，４４×１０￣７个体／Ｌ，ＭＴＳＩ值四季分别为４．２７、５．１１、５．２６、４．９６．这表明水体已受到中度污染，达到中富和富营养水平，且表现出逐季增加的趋势。

关键词：浮游植物富营养化水质网箱养殖

Assessing mariculture water quality with the structural and functional characteristics of a ciliate community

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Chinese Journal of Oceanology and Limnology 2011年第1期29卷 128-135页

作者：李继秋许恒龙林晓凤 AL-RASHEID Khaled A.S. Laboratory of Protozoology Key Laboratory of Ecology and Environmental Science in Guangdong Higher EducationSouth China Normal University Laboratory of Protozoology Institute of Evolution and Marine biodiversityOcean University of China Zoology Department King Saud University

Ciliated protozoa play important roles in micro-ecosystems, especially in marine biotopes. However, few studies have been carried out on the periphytic, or aufwuch, forms in mariculture waters so far. In this study, we sampled periphytic ciliate communities in two closed mariculture ponds (ponds CP1 and CP2) and a natural seawater reservoir (pond RP) using a glass slide method to evaluate their colonizing processes and general ecological features, as well as their application as water quality indicators. We analyzed species compositions, structural parameters (species number, richness, diversity, evenness, abundance and dBP) and functional parameters (G, Seq and T90%). Pond RP was characterized by higher levels of structural parameters (except for abundance and dBP) and more equal proportion of the major taxonomic groups. The values of Seq were significantly higher in pond RP and similar in both pond CP1 and CP2. It was also demonstrated that environmental factors, including NO2-H, NO3-H, NH3-H, soluble reactive phosphate, temperature and pH, were the first principal factors affecting the communities. Among them, temperature and chemical factors were all significantly and negatively correlated with species number (P<0.01), richness (P<0.01), diversity (P<0.01), and positive correlated with abundance (P<0.01). Opposite correlations between pH and structural parameters were observed. This study showed that there were significant differences in species composition, structural parameters and functional parameters of the periphytic ciliate communities among the ponds, which were in agreement with the water quality. Results of this study confirmed the periphytic ciliate communities to be useful bioindicators of water quality in intensive mariculture waters.

关键词： periphytic ciliates community structure environmental factors bioindicators

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进化论与生物学的关系刍议

聊城大学学报（自然科学版） 1996年第1期14卷 87-88,92页

作者：张丽娟许恒龙杨敏一聊城八中聊城师范学校

对进化论与生物学的关系进行了浅析．达尔文进化论的建立使生物学各分支学科成为彼此相互关联系、相互印证的统一体系；推进生物学发生了革命性的变革，同时，生物学的各个分支，为生物进化过程提供了科学证据．

关键词：进化论生物学关系达尔文

海洋纤毛虫实验生态学研究Ⅰ:纤毛虫对氨积累的影响

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海洋纤毛虫实验生态学研究Ⅰ:纤毛虫对氨积累的影响

Population dynamics of marine ciliate Euplotes vannus (Protozoa, Ciliophora) in different artificial seawaters

中国动物学会原生动物学分会第十二次学术讨论会

作者：许恒龙宋微波朱明壮中国海洋大学原生动物学研究室中国海洋大学原生动物学研究室中国海洋大学原生动物学研究室

＜正＞为探讨纤毛虫原生动物在海水养殖环境中的生态学功能,利用实验生态学方法,对海水养殖水体中两种常见纤毛虫:扇形游仆虫（Euplotes vannus Muller）和海洋尾丝虫（Uronema marinum Dujardin）在米粒和牛肉浸膏两种培养液中的种群... 详细信息

＜正＞为探讨纤毛虫原生动物在海水养殖环境中的生态学功能,利用实验生态学方法,对海水养殖水体中两种常见纤毛虫:扇形游仆虫（Euplotes vannus Muller）和海洋尾丝虫（Uronema marinum Dujardin）在米粒和牛肉浸膏两种培养液中的种群生长过程与氨氮浓度之间的关系进行了初步研究。实验共持续14天,期间对各培养中

关键词：纤毛虫原生动物细胞毒理氨降解

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Chinese Journal of Oceanology and Limnology 2011年第1期29卷 109-117页

作者：许恒龙朱明壮姜勇高珊 MIN Gi-Sik AL-RASHEID Khaled A.S. Laboratory of Protozoology Institute of Evolution and Marine BiodiversityOcean University of China Department of Biological Sciences Inha University Zoology Department King Saud University

To study population dynamics of marine ciliates in different artificial seawaters （ASW）, the population growth dynamics of a common marine ciliate Euplotes vannus were investigated using beef extract media and rice media for five types of ASW and natural seawater （NSW）. The results show that：（1） the population growth rate was in the order of NSW〉Flack ASW〉Nakamula ASW〉Schmadz ASW〉Oshima ASW〉Subow ASW and was considerably higher in rice media than in beef extract media （apart from Subow ASW）; （2） the maximum density of E. vannus in stationary phase in each treatment was ranked as Hack ASW〉Nakamula ASW〉Schmadz ASW〉NSW〉Oshima ASW〉Subow ASW, and was again higher in rice media than in beef extract media （except for Subow ASW）; （3） the exponential and stationary phases were longer in rice media than in beef extract media; （4） strains of E. vannus that had been domesticated for 〉1 year in ASW grew significantly slower, with lower maximum density and longer stationary phase than those isolated and maintained in NSW. It was demonstrated that：（1） E. vannus may grow well in Flack, Nakamula and Schmads ASW compared with NSW （mainly in terms of growth rate）; and （2） Oshima ASW is the preferred choice for stock cultures of E. vannus, but the ASWs Flack, Nakamula and Schmadz are preferred for mass culture. These findings suggest that these three ASWs are effective for the cultivation of marine protozoa for experimental studies on ecology, toxicology and molecular biology.

关键词： artificial seawater culture ecology marine ciliate population dynamics

凯氏聚缩虫(Zoothamnium kentii Grenfell,1884)形态学及表膜下纤维系统的研究

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聊城大学学报（自然科学版） 2005年第3期18卷 51-54页

作者：齐桂兰施心路许恒龙聊城大学生命科学学院山东聊城252059 哈尔滨师范大学生命与环境科学学院

利用活体观察和蛋白银染色技术对凯氏聚缩虫的形态学和表膜下纤维系统进行了研究. 该种的主要鉴别特征为:群体呈不规则的双叉型分支;个员完全伸展时宽钟形,体长60-80μm, 体宽40～50μm,口围盘宽40-50μm;伸缩泡单一,顶位;大核“C”形,... 详细信息

利用活体观察和蛋白银染色技术对凯氏聚缩虫的形态学和表膜下纤维系统进行了研究. 该种的主要鉴别特征为:群体呈不规则的双叉型分支;个员完全伸展时宽钟形,体长60-80μm, 体宽40～50μm,口围盘宽40-50μm;伸缩泡单一,顶位;大核“C”形,横位;P2和P3终止后P1继续向胞口的深处延伸,但末端较为平直并不发生明显的弯曲,三列毛基索在终止处向外分散;口围盘纤维碟状;纵长纤维较粗,整体呈现网状.

关键词：缘毛目纤毛虫凯氏聚缩虫形态学表膜下纤维系统

Temporal population dynamics of dinoflagellate Prorocentrum minimum in a semi-enclosed mariculture pond and its relationship to environmental factors and protozoan grazers

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Chinese Journal of Oceanology and Limnology 2010年第1期28卷 75-81页

作者：许恒龙 MIN Gi-Sik CHOI Joong-Ki 朱明壮姜勇 AL-RASHEID Khaled A.S. Laboratory of Protozoology KLMOcean University of China Department of Biological Sciences Inha University Department of Oceanography Inha University Zoology Department King Saud University

The ecological processes and interrelationships between protists,either autotrophic or heterotrophic,and environmental factors in mariculture ponds are largely *** study investigated the temporal dynamics of potentially harmful dinoflagellate,Prorocentrum minimum (Pavillard) Schiller,and its relationship to physico-chemical factors and protozoan grazers over a complete cycle in a semi-enclosed shrimp-farming pond near Qingdao,Northern *** occurred frequently in low numbers from June to August,followed by a sharp increase from the middle of August,reaching a single maximum peak value of 2.2×105 cells L-1 in *** variation in abundance was positively correlated with dissolved nitrogen,but showed a significant inverse relationship to abundance of the dominant ciliates,Tintinnopsis lohmanni and Askenasia *** results provide statistical evidence that the number of *** increased with increasing nitrogen,and the suppression or shortening of algal bloom may be associated with protozoan grazers,such as Tintinnopsis lohmanni,in mariculture ponds.

关键词： dinoflagellate harmful algae Prorocentrum minimum shrimp-farming