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不同剂量和剂型的氟伐他汀对代谢综合征小鼠糖代谢的影响

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中国药学杂志 2012年第22期47卷 1821-1824页

作者：张雪娟宋力平立风孙希美李国梁青岛大学医学院附属医院心内科山东青岛266003

目的探讨不同剂量和剂型的氟伐他汀对代谢综合征(metabolic syndromes,MS)小鼠胰岛素抵抗(insulin resistance,IR)及超敏C反应蛋白(hs-CRP)的影响。方法 60只雄性SPF级KM小鼠随机分为2组,正常对照组10只,模型组50只,正常对照组给予正常... 详细信息

目的探讨不同剂量和剂型的氟伐他汀对代谢综合征(metabolic syndromes,MS)小鼠胰岛素抵抗(insulin resistance,IR)及超敏C反应蛋白(hs-CRP)的影响。方法 60只雄性SPF级KM小鼠随机分为2组,正常对照组10只,模型组50只,正常对照组给予正常饮食,模型组给予高热量饮食,4周末模型组随机抽取10只,检测MS模型建立,第5周开始剩余40只随机分为A、B、C、D 4组,均给予正常饮食,分别给予氟伐他汀胶囊6 mg·kg-1·d-1,氟伐他汀胶囊12 mg·kg-1·d-1,氟伐他汀缓释片12 mg·kg-1·d-1,生理盐水灌胃,8周末检测血脂(TC、TG、LDL-C、HDL-C)、hs-CRP、空腹血糖(FBG)、血清胰岛素(FINS),以及计算稳态模型胰岛素抵抗评价指数(homeostasis model assessment of insulin resistance,HOMA-IR)。结果 4周末计算模型组HOMA-IR增高,即形成胰岛素抵抗,成功建立代谢综合征模型,8周末A、B、C 3组与D组比较,TC、TG和LDL-C水平均明显降低(P平显著升高(P平也明显下降(P平下降更为显著(P平则无明显变化;而C组与B组相比,TC、TG、LDL-C和hs-CRP、FBG、FINS水平均明显降低(P<0.05),HOMA-IR也明显下降(P<0.05)。结论氟伐他汀可降低MS小鼠TC、TG、LDL-C、FINS、hs-CRP,改善IR,且随剂量的增加和剂型的改善作用更为明显。其作用机制可能与抗炎作用有关。

关键词：氟伐他汀代谢综合征胰岛素抵抗超敏C反应蛋白

甲基莲心碱调节SDF-1/CXCR4信号通路对糖尿病肾病的影响

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上海交通大学学报（医学版） 2024年第2期44卷 183-195页

作者：王莹平立风刘彤彤刘珊珊刘磊山东第一医科大学第二附属医院内分泌科泰安271000 山东第一医科大学第二附属医院全科医学科泰安271000 山东第一医科大学第二附属医院心血管内科泰安271000 山东省泰安市中心医院妇科泰安271000

目的·探讨甲基莲心碱(neferine,Nef)对糖尿病肾病(diabetic nephropathy,DN)大鼠肾组织的作用及其相关机制。方法·采用高脂饲料喂食联合腹腔注射链脲佐菌素的方法构建DN模型大鼠,并将造模成功的大鼠随机分为DN组、Nef(低、中、高)剂... 详细信息

目的·探讨甲基莲心碱(neferine,Nef)对糖尿病肾病(diabetic nephropathy,DN)大鼠肾组织的作用及其相关机制。方法·采用高脂饲料喂食联合腹腔注射链脲佐菌素的方法构建DN模型大鼠,并将造模成功的大鼠随机分为DN组、Nef(低、中、高)剂量组、Nef高剂量+通路拮抗剂(AMD3100)组,每组10只。同时,选10只普通大鼠作为正常组。检测6组大鼠的空腹血糖(fasting blood glucose,FBG)、24 h尿蛋白、血清糖化血红蛋白(glycosylated hemoglobin,HbA1c)、血清肌酐(serum creatinine,Scr)、尿素氮(blood urea nitrogen,BUN)水平及肾指数。分别采用苏木精-伊红(hematoxylin-eosin,H-E)染色、马松(Masson)染色观察6组大鼠的肾组织的病理变化。采用硫代巴比妥酸(thiobarbituric acid,TBA)法检测肾组织丙二醛(malondialdehyde,MDA)含量,分别采用水溶性四氮唑(water soluble tetrazolium,WST-1)法、钼酸铵法检测肾组织超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)的活性。分别采用实时荧光定量PCR(quantitative real-time PCR,qPCR)和蛋白质印迹法(Western blotting)检测肾组织中基质细胞衍生因子-1(stromal cell-derived factor-1,SDF-1)、CXC趋化因子受体4(CXC chemokine receptor 4,CXCR4)的mRNA以及蛋白表达。采用高糖(30 mmol/L葡萄糖)诱导大鼠肾小管上皮细胞NRK-52E,以建立DN细胞模型。将该细胞分为对照组、高糖(HG)组、HG+Nef(低、中、高)剂量组(即HG+Nef-L、M、H组)、HG+Nef-H+AMD3100组。分别采用WST-1法、钼酸铵法检测模型细胞中SOD、CAT活性,采用TBA法检测MDA含量,分别采用qPCR、Western blotting检测SDF-1、CXCR4的mRNA及蛋白表达,采用CCK-8法、流式细胞术检测细胞活力和凋亡率。结果·与DN组比较,Nef(低、中、高)剂量组和Nef高剂量+AMD3100组大鼠的FBG、24 h尿蛋白、HbA1c、Scr、BUN水平以及肾指数、MDA水平均较低,SDF-1、CXCR4的mRNA和蛋白表达以及SOD、CAT活性均较高(均P平并提高其抗氧化能力,从而发挥肾保护作用。

关键词：糖尿病肾病甲基莲心碱肾脏基质细胞衍生因子-1/CXC趋化因子受体4信号通路

Protective effect of Xuezhikang capsules in rats with acute myocardial ischemia reperfusion injury

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South China Journal of Cardiology 2012年第1期13卷 40-45页

作者：张雪娟宋立滕蕾平立风 Department of Cardiology Affiliated Hospital of Qingdao University Medical College Department of Anesthesiology Affiliated Hospital of Qingdao University Medical College Qingdao University Medical College

Background Recent researches have found that stains can improve acute myocardial ischemia reperfusion injury which is achieved by inhibiting inflammatory *** is extracted from red rice,a tailor-made Chinese crude *** component of Xuezhikang that can inhibit blood-fat is *** Forty healthy SD rats （half male and half female,200 g or so） were randomly divided into four groups：A：normal control;B：sham operation;C：MIR group;D：Xuezhikang *** acute myocardial ischemia reperfusion injury model was *** sizes,MYO,CK-MB,cTnI,IL-10 and IL-18 were detected after *** Compared with C and D group,in A and B group,infarct size were increased significantly （P 0.01）,the level of serum MYO,CK-MB,cTnI were increased significantly （P 0.01）,the level of IL-10 were decreased significantly （P 0.01） and IL-18,CRP were increased significantly （P 0.01）.Compared with C group,infarct size were decreased significantly （P 0.05）,the level of serum MYO,CK-MB and cTnI were increased significantly（P 0.05）,the level of IL-10 were increased significantly （P 0.05） and IL-18 were decreased significantly （P 0.05）.The level of IL-10 and IL-18 were no difference between A and B *** The application of Xuezhikang capsules on rats before the operation of myocardial ischemia reperfusion can lessen inflammatory reaction and reduce infarct sizes and protect acute myocardial ischemia reperfusion.

关键词： Xuezhikang capsule rats myocardium ischemia-reperfusion endothelial function

Effects of atorvastatin on cardiovascular remodeling in 2-kindey and 1-clip hypertensive rats

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South China Journal of Cardiology 2011年第4期12卷 257-261页

作者：平立风张雪娟宋立谭凯 Qingdao University Medical College Department of Cardiology Affiliated Hospital of Qingdao University Medical College Department of Anesthesiology Affiliated Hospital of Qingdao University Medical College municipal hospital

Background Nowadays, the studies mainly focus on the function of decreasing the inflammatory factor and improving the functions of endothelium, but the Methods The 2-kindey, 1-clip hypertensive rats effects of statins on ventricular remodeling are rarely studied. （2K1C, Goldblatt） were prepared with Sprague-Dawley （SD） rat. SD rats were randomly divided into three groups： control rats, hypertensive rats and hypertensive rats treated with atorvastatin （2 mg·kg^-1·d^-1）. After 6 weeks, systolic blood pressure （SBP） was measured using the tail-cuff method. The plasma concentration of angiotensin Ⅱ and renin activity were determined by radioimmunoassay. The heart weight, the ratio of left ventricular weight and body weight was calculated. Results The plasma concentration of angiotensin Ⅱ （106.4±7.8） ng/L and renin activity （20.6±2.4） ng/L were significantly increaed in hypertensive rats compared with normal rats [ （72.3±5.4） ng/L and （12.5±3.7） ng/L] （P 〈 0.01）. The heart weight （1.46 ± 0.09）g, the ratio 3.54±0.19 （ × 10^-3） of left ventricular weight and body weight in hypertensive rats were obviously higher than that in normal rats [（0.98±0.07）g and （2.28 ± 0.06） × 10^-3] （P 〈 0.01）. After treatment with atorvastatin, the plasma concentration of angiotensin Ⅱ （68.3 ± 6.9） ng/L and renin activity（8.7 ± 2.3 ）ng/L, heart weight（1.05± 0.04）g, the ratio 2.36 ± 0.07 （×10^-3） above were decreased significantly, there were no difference between the group of hypertensive rats and the normal. Conclusions Atorvastatin can decrease the ratio of left ventricular weight and body weight and has the effects on cardiovascular remodeling in hypertensive rats.

关键词： atorvastatin renovascular hypertensive left ventricle remodeling