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文献详情 >TcpC is not only an E3 ubiquit... 收藏
TcpC is not only an E3 ubiquitin ligase but also an inhibito...

TcpC is not only an E3 ubiquitin ligase but also an inhibitor of USP14 deubiquitinase

作     者:Jiaqi Fang Qian Ou Xinpeng Ma Weiwei Luo Wenting Li Sisi Li Jiajie Jing Ziyan Jiang Jiadong Wang Weiyu Jiang Jianping Pan 

作者单位:Institute of Translational MedicineHangzhou City University Department of Clinical MedicineHangzhou City University School of Medicine Department of Basic Medical SciencesZhejiang University School of Medicine 

会议名称:《浙江省免疫学会第十三次学术大会》

会议日期:1000年

学科分类:1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

关 键 词:TcpC inhibit deubiquitinase USP14 innate immune 

摘      要:Background:Toll/interleukin 1 receptor domain containing protein encoded by ***(TcpC) is a multifunctional virulence factor in the majority of uropathogenic ***(UPEC) strains and blocks the TLR signaling pathway by serving as an E3 ubiquitin ligase that promotes MyD88 degradation by ubiquitin-proteasome pathway,hereby inhibiting the TLR mediated activation of ***:This study aims to investigate if TcpC can also inhibit the activity of deubiquitinase and its related *** and methods:TcpC secreting wild-type CFT073(CFT073) was kindly provided by Professor Jian-guo *** knock out CFT073 mutant(CFT073Δtcpc) was constructed in our *** human monocytic cell line THP-1 and mouse macrophage cell lines J774 A.1 were obtained from Shanghai Institute of Biochemistry and Cell *** situ co-localization of TcpC with USP14,USP14 with ubiquitin and Rpn1 in kidneys from pyelonephritis mouse model was determined by confocal *** of UPEC in macrophages was observed by holographic 3 D live cell imaging system,flow cytometry and confocal *** of deubiquitinase and proteasome were measured by fluorescent microplate reader.K48-ubiquitination of MyD88 in rTcpC treated macrophages was detected by *** mRNA and protein expression levels of pro-inflammatory cytokines were detected by qRT-PCR and *** abilities of rTcpC with rUSP14 was determined by surface plasmon resonance(SPR) and microscale thermophoresis(MST).Transcription and protein level of ubiquitin and proteasome related genes were detected by transcriptome and proteome *** amino acids required to maintain the deubiquitinase inhibitory activity in rTcpC were identified by ***:In situ co-localization of TcpC and ubiquitin with USP14 or PSMD2 with USP14 was significantly enhanced or declined respectively in kidney from CFT073 infected mice compared to kidney isolated from mice infect

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