GLYR1 could regulate transcription of PER3 and affect the growth and regulate bortezomib sensitivity of multiple myeloma cells
作者单位：Department of HaematologyThe First Affiliated Hospital of Ningbo University
会议名称：《浙江省免疫学会第十三次学术大会》
会议日期：1000年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
关 键 词：multiple myeloma period circadian regulator 3 glyoxylate reductase 1 homolog apoptosis
摘 要：Background:Period circadian regulator 3(PER3) functions as a tumor suppressor in various ***,the roles of PER3 in multiple myeloma(MM) have not been ***:This study aimed to investigate the effects of PER3 on MM cell growth and bortezomib *** and methods:RT-qPCR was applied for determining mRNA expression and western blot for detecting protein *** binding sites of glyoxylate reductase 1 homolog(GLYR1) on the promoter of PER3 was analyzed by UCSC and confirmed by luciferase and Chromatin immunoprecipitation(ChIP) *** behaviors were determined by CCK-8,colony formation,TUNEL,as well as transwell ***:The expression of PER3 in choroid plexus,placenta,appendix,bone marrows and thymus,especially in bone marrows,was relatively ***,we further analyzed PER3 expression in various ***3 expression was reduced in *** we analyzed PER3 expression in MM *** found that PER3 expression,generally,was low in MM ***,bioinformatics analysis indicated that low expression of PER3 in MM patients showed significantly poor 5-year overall survival(OS) rates and event-free survival(EFS) rates compared with MM patients with highly expressed *** of PER3 suppressed the proliferative,migrative,and invasive ability of MM cells,and also increased the sensitivity of MM cells to ***,results of bioinformatic analysis suggested that GLYR1 was able to regulate the transcription of PER3,meanwhile,overexpressed GLYR1 significantly increased the mRNA expression of ***,PER3 was markedly enriched by the treatment of ***,co-transfection with PER-WT and GLYR1 overexpression plasmids markedly increased luciferase activity,while PER-MT had no significant effects on luciferase *** results suggested that GLYR1 can regulate the transcription of ***,overexpressed GLYR1 significantly decreased the viability,migration,and in