Nuclear Factor AP2X-4 Governs Toxoplasma Infection and Virulence by Regulating the Expression of Rhoptry Proteins
作者单位：State Key Laboratory of Agricultural MicrobiologyCollege of Veterinary MedicineHuazhong Agricultural University Key Laboratory of Preventive Medicine in Hubei Province
会议名称：《2021中国畜牧兽医学会兽医寄生虫学分会第16次学术研讨会》
会议日期：2021年
学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学]
摘 要：Toxoplasma gondii is a ubiquitous pathogen infecting one third of the world s population and diverse *** has a complex life cycle alternating among different developmental stages,which contributes to its transmission and *** parasite has a sophisticated gene regulation network that enables timely expression of genes at designated ***,little is known about the underlying regulatory *** we identified an AP2 family transcription factor named TgAP2 X-4,which was crucial for tachyzoite(parasite at the acute infection stage)*** or conditional depletion of TgAP2 X-4 severely reduced parasite growth in vitro,which is explained by significantly decreased efficiency of host cell invasion and a modest reduction of intracellular *** analyses suggested that TgAP2 X-4 inactivation resulted in impaired expression of many proteins that traffic to the rhoptry organelle,including RON2,RON4 and RON8 that are key for parasite *** addition,TgAP2 X-4 also regulates a subset of genes that are normally induced during chronic infection,such as BAG1 and *** the diverse targets of TgAP2 X-4,its inactivation led to drastic attenuation of parasite virulence and complete inability to form chronic ***2 X-4 is the first described AP2 factor to be crucial for tachyzoite invasion and growth,and it represents a candidate for antitoxoplasmic drug and vaccine designs.