Deciphering the genetic architecture of anther culturability in rice using segregation distortion approach
作者单位：Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province) Ministry of Agriculture and Rural Affairs Crop Breeding and Cultivation Research Institute Shanghai Academy of Agricultural Sciences MOE Key Laboratory of Crop Physiology Ecology and Genetic Breeding College of Agronomy Jiangxi Agricultural University
会议名称：《第二十届中国作物学会学术年会》
会议日期：2023年
摘 要：Anther culture is a promising technique used in rice breeding, although the genetic mechanisms associated with anther culturability remain elusive. In the process of in vitro anther culture, favorable alleles for anther culture are preferentially transmitted to double haploid(DH) populations, resulting in biased segregation of DH populations. Identified five potential loci(LOD3.5) that may be associated with anther culturability using the segregation distortion method, including SDL2 and SDL3, which are overrepresented in japonica alleles, and SDL7, SDL9, and SDL11, which are overrepresented in indica alleles. Additionally, independent segregation analysis revealed significant epistatic interactions between SDL2 and SDL3, as well as eight pairs of epistatic interaction loci(P0.001). These findings suggest that anther culturability in rice is governed by complex genetic mechanisms involving multiple nuclear genes and epistatic interactions. This study provides insights into the genetic control underlying anther culturability in rice and lays the foundation for future research aimed at identifying causal genes associated with anther culturability.