Liver proteomics analysis reveals abnormal metabolism of bile acid and arachidonic acid in Chinese hamsters with type 2 diabetes mellitus
作者单位：Laboratory Animal CenterShanxi Key Laboratory of Experimental Animal Science and Animal Model of Human DiseaseShanxi Medical University School of Basic MedicineBasic Medical Science CenterShanxi Medical University Department of CardiologySecond Hospital of Shanxi Medical University Department of PharmacologyShanxi Medical University Jingjie PTM BioLab (Hangzhou) Co.LtdNo.4526th Street Hangzhou Eco.&Tech.Developmental Area
会议名称：《第十六届中国实验动物科学年会》
会议日期：2023年
学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
摘 要：Non-obese,spontaneous,and genetically predisposed type 2 diabetic Chinese hamsters exhibit metabolic abnormalities similar to those observed in human T2 ***,tandem mass tag(TMT)-based quantitative proteomics technology was used to screen and identify differentially abundant proteins in the liver that are associated with diabetes in Chinese *** and KEGG pathway enrichment analysis were conducted to validate the findings,as well as qRT-PCR and western *** total,103 proteins were identified in the livers of diabetic hamsters,of which 48 were up-regulated and 55 were *** *** analysis further demonstrated that linoleic acid metabolism,arachidonic acid metabolism,bile secretion,and other pathways were ***,AQP9 and EPHX1 were significantly down-regulated in the bile secretion pathway,whereas PTGES2,Cyp2 c27,and Cyp2 c70 were associated with the arachidonic acid metabolic *** levels of bile acid(BA) and arachidonic acid(AA) in diabetic Chinese hamsters were significantly higher than those in control ***,our findings indicate that the five candidate proteins may be associated with abnormal BA and AA metabolism,suggesting their involvement in pathological changes in the livers of Chinese hamsters with T2 DM.