Oestrogen-regulated super-enhancer-associated lncRNA NCALD plays a key role in luminal breast cancer progression by forming a complex with ERa to activate GRHL2
作者单位：guangdong provincial peoples hospital
会议名称：《2022CCTB中国肿瘤标志物学术大会暨中国整合肿瘤学大会暨第十六届肿瘤标志物青年科学家论坛暨中国肿瘤标志物产业创新大会》
会议日期：2023年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
摘 要：Background:Luminal breast cancer is one of the most popular subtype,which has a sustained risk of late disease recurrence and ***-enhancer assoicated lncRNAs may play prominent roles in driving tissue-specific gene expression in the ***,the biological function of specific transcription dysregulated SE-lncRNAs in luminal breast cancer and the underlying mechanisms are still largely ***:The ChIP-seq data,RNA-seq and Hi-C data were used to analyze super-enhancer regulated SE-lncRNA *** ChIP-seq data,ChIP-qPCR,EMSA,supershift-EMSA and Dual-luciferase reporter assay were conducted to explore the transcription of SE-lncRNA NCALD activated by estrogen receptor(ERα).RT-qPCR was used to detect the expression of SE-lncRNA NCALD,RT-qPCR and Western blotting were used to determine the expression of ERα and *** pull-down and RIP were employed to investigate the interaction between SE-lncRNA NCALD and ERα.FISH,ChIP-qPCR were used to elucidate the interaction between SE-lncRNA NCALD ERα and *** effect of SE-lncRNA NCALD on malignant phenotypes was examined through in vitro and in vivo ***:To identify the significant overexpressed SE-lncRNAs in luminal breast cancer,three pairs of luminal breast cancers and para-carcinoma tissues were examined by a SE-lncRNA ***-lncRNA NCALD was chosen owing its significant specifically overexpressed in luminal breast *** found that The expression level of SE-lncRNA NCALD is regulated by super-enhancers(SEs) driven by ERα.High expression levels of SE-lncRNA NCALD was associated with poor *** experiments showed SE-lncRNA NCALD was essential for the growth of ***,we demonstrated SE-lncRNA NCALD can regulate the expression of the master transcriptional factor GRHL2 by recruitment of ERα in the promoter of ***:Our study demonstrated that SE-lncRNA NCALD was transcriptionally activated by super-enhancers driven by ERα and exhibited l