The oncomicroprotein MUCP1 promotes colorectal cancer progression by regulating lipid metabolism
会议名称：《2022CCTB中国肿瘤标志物学术大会暨中国整合肿瘤学大会暨第十六届肿瘤标志物青年科学家论坛暨中国肿瘤标志物产业创新大会》
会议日期：2023年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
摘 要：Background:Long non-coding RNAs(lncRNAs) exercise critical roles in cancer development and *** purpose of this study is to identify aberrantly expressed lncRNAs in colorectal cancer(CRC) and provide new targets for CRC diagnosis and ***:Ribosome profiling sequencing(Ribo-seq) were performed to identify lncRNAs capable of binding to ribosomes,and from which LINC00969 was selected for elevated expression in *** construct,Mass spectrometry,Immunofluorescence,Co-immunoprecipitation,and Western blot were used to verify lncRNA-encoded microprotein,and we named it ***-and loss-of-function studies were performed to examine the biological roles of MUCP1 in CRC *** sequencing,qRT-PCR,Western blot and Cellular cholesterol levels assay were performed to analyze MUCP1-regulated downstream target genes and signaling *** derived xenograft were used to analyze the effects of MUCP1 on CRC ***:LINC00969 was up-regulated in CRC tissues and its elevated levels were associated with distal metastasis and poor ***,LINC00969 encoded a novel microprotein MUCP1 that directly interacted with SCD1 to facilitated cholesterol synthesis,and which led to the activation of MAPK signaling ***1 knockdown inhibited while overexpression promoted CRC cell migration and ***1 overexpression promoted CRC growth and metastasis in mouse tumor ***:MUCP1 is a new oncomicroprotein that has tumor promoting effect in CRC and it may serve as a potential prognostic biomarker and therapeutic target for CRC.