BNIP3 mediated mitophagy attenuates intermittent hypoxia-induced human renal tubular epithelial cells injury
作者单位：Department of Respiratory Medicine Second Affiliated Hospital of Fujian Medical University 福建医科大学附属第一医院
会议名称：《中国睡眠研究会第十四届全国学术年会》
会议日期：2022年
学科分类：1002[医学-临床医学] 100213[医学-耳鼻咽喉科学] 10[医学]
摘 要：Background:As one novel pathophysiological characteristic of obstructive sleep apnea, intermittent hypoxia(IH) contributed to human renal tubular epithelial cells impairment. The underlying pathological mechanisms remain unrevealed. The present study aimed to evaluate the influence of Bcl-2 19-kDa interacting protein 3(BNIP3) mediated mitophagy on IH-induced renal tubular epithelial cell *** and methods:Human kidney proximal tubular(HK-2) cells were exposed to IH condition. IH cycles were as follows: 21% oxygen for 25 min, 21% descended to 1% for 35 min, 1% oxygen sustaining for 35 min, 1% ascended to 21% for 25 min. The IH exposure lasted 24 h with 12 cycles of hypoxia and re-oxygenation. Both the siBNIP3 and BNIP3 vector were transfected to cells. Cell viability and apoptosis, mitochondrial morphology and function, mitophagy were detected by cell counting kit-8, flow cytometry and TUNEL staining, transmission electron microscope, western blotting, and immunofluorescence, ***:In the IH-induced HK-2 cells, inhibition of BNIP3 further aggravated mitochondrial structure damage, decreased mitophagy level, leading to increased cell apoptosis and decreased cell viability. While overexpression of BNIP3 enhanced mitophagy, which protected mitochondrial structure, can decrease cell death in HK-2 cells exposed to ***:The present study showed that BNIP3-mediated mitophagy plays a protective role against IH-induced renal tubular epithelial cells impairment.