Developing an isotope dilution UHPLC-MS/MS method to quantify linezolid in human plasma: application to therapeutic drug monitoring
作者单位：Department of Pharmacy Sun Yat-Sen Memorial Hospital Sun Yat-Sen University
会议名称：《2021第26届广东省药师周大会》
会议日期：2020年
学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学]
关 键 词：Linezolid LC-MS/MS Therapeutic drug monitoring(TDM)
摘 要：Aim: To optimize clinical efficacy and reduce the drug-exposure-related toxicity of linezolid, whose concentrations show wide inter-variabilities, a simple and reliable quantitative assay for TDM is necessary. Results: A UHPLC-MS/MS assay has been established for determination of linezolid in human plasma and fully validated according to the FDA guidelines. After a simple, isotope-dilluted precipitation with methanol, the analytes were separated by a straightforward isocratic mode and the tandem mass spectrometry was conducted under the ESI+ mode fitted with SRM. The calibration curves proved acceptable linearity in the range of 0.1-30.0 ug/mL. Conclusion: The present assay is currently used in routine clinical practice, being applied to TDM and helps to optimize individual dosing regimens and manage adverse effects in ICU patients.