A multiplex reverse transcription PCR assay for simultaneous detection of five main RNA viruses and in nine curcubit plants
作者单位：Institute of Plant Inspection and QuarantineChinese Academy of Inspection and Quarantine
会议名称：《中国植物病理学会2021年学术年会》
会议日期：2021年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学]
关 键 词：plant virus detection RNA viruses curcubit plants PCR
摘 要：Virus and bacteria diseases occur in cucurbit fruits and vegetables all around the world,causing serious yield *** rapid detection of these pathogens and provide a basis for disease control,a multiplex reverse transcription polymerase chain reaction system was established for simultaneous detection of Tobacco mosaic virus（TMV）,Zucchini yellow mosaic virus（ZYMV）,Cucumber mosaic virus（CMV）,and in 9 different cucurbit plants,with 5 pairs of specific primers being designed based on the coat protein（CP） genes of these *** elongation factor-1α（EF-1α） from tomato was added to the multiplex RT-PCR reaction system toprevent false *** concentration of the primers,annealing temperature,annealing time,extension time and amplification cycles were *** fragments of 159 bp（ToCV）,262 bp（PVY）,362 bp（EF-1α）,430 bp（TMV）,500 bp（TSWV）,600 bp（CMV） and 705 bp（PVX） were amplified by this multiplex RT-PCR system,and their origin was confirmed by DNA *** method will have a wide application in virus detection of field samples.