A predatory soil bacterium reprograms a quorum sensing signal system to regulate antifungal weapon production in a cyclic-di-GMP-independent manner
作者单位：College of Plant ProtectionNanjing Agricultural University Institute of Plant ProtectionJiangsu Academy of Agricultural Sciences
会议名称：《中国植物病理学会2021年学术年会》
会议日期：2021年
关 键 词：DSFs PDE HyTCS Quorum sensing signal system
摘 要：Soil bacteria often provide multiple weapons to use against eukaryotes or prokaryotes *** signal factors（DSFs） represent a unique group of quorumsensing（QS） chemicals that modulate interspecies competition inbacteriathat do not produce antibiotic-like ***,themolecular mechanism by which DSF-mediated QS systems regulate weapons production for interspecies competition remains largely unknown in soil biocontrol *** this study,we found that the necessary QS system component protein RpfG from Lysobacter,in addition to being a cyclic dimeric GMP（c-di-GMP） phosphodiesterase（PDE）,regulates the biosynthesis of an antifungal weapon（heatstable antifungal factor,HSAF）,which does not appear to depend on the enzymatic ***,we showed for the first time that RpfG interacts with three hybrid two-component system（HyTCS） proteins,HtsHl,HtsH2,and HtsH3,to regulate HSAF production in *** vitro studies showed that each of these proteins interacted with RpfG,which reduced the PDE activity of ***,we showed that the cytoplasmic proportions of these proteins depended on their phosphorylation activity andbinding tothe promoter controlling the genes implicated in HSAF *** findings reveal apreviously uncharacterized mechanism of DSF signalling in antifungal weapon production in soilbacteria.