A NIS1-like protein from apple canker pathogen（Valsa mali）may escape plant recognition and serves as a virulence factor
作者单位：State Key Laboratory of Crop Stress Biology for Arid AreasCollege of Plant ProtectionNorthwest A＆F University Department of Plant PathologyChina Agricultural University
会议名称：《中国植物病理学会2021年学术年会》
会议日期：2021年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学]
关 键 词：Valsa mali NIS1-like protein cell death immune responses virulence
摘 要：Phytopathogens-produced conserved effectors play important roles in plant-microbe ***1-like proteins represent a newly identified family of effectors represented in a wide array of fungal ***,their biological roles in pathogenic fungi remain largely *** this study,we characterized two NIS1-like proteins VmNISl and VmNIS2 from Valsa mali,the causal agent of apple Valsa *** found that VmNISl induced intense cell death,whereas VmNIS2 suppressed INF1 elicitin-triggered cell death when transiently expressed in the model plant Nicotiana *** of *** with VmNISl recombinant protein activated a series of immune responses and greatly enhanced plant resistance against Phytophthora *** contrast,VmNIS2 strongly suppressed plant immune responses and promoted the infection of *** when transiently expressed in *** observations indicate that VmNISl but not VmNIS2 can be recognized by *** alignment revealed that,compared to VmNISl,VmNIS2 is truncated at Cterminus by 13 amino acids residues（C13）.Interestingly,deletion of C13 greatly compromised VmNIS1-triggered cell death,whereas fusion of C13 to the C-terminus of VmNIS2 can induce visible cell death in *** indicates that C13 is essential for plant detection of VmNISl,and VmNIS2 may evade plant recognition via C-terminal *** further found that knocking out of VmNIS1 showed no apparent influence on *** virulence,however,knocking out of VmNIS2 obviously attenuated pathogen virulence,suggesting that VmNIS2 is essential for *** infection of *** conclusion,our data suggest that the NIS1-like protein VmNIS2 from the pathogenic *** may have escaped plant recognition and functions as a virulence factor.