SNR enhancement of low voltage cryo-EM
作者单位：中国科技大学生命科学院 中科院生物物理所
会议名称：《第七届全国冷冻电子显微学与结构生物学专题研讨会》
会议日期：2021年
学科分类：071011[理学-生物物理学] 0710[理学-生物学] 07[理学]
摘 要：正For small proteins,the main limiting factor is that their mass is so small that the ability to scatter electrons is significantly lower,resulting in relatively low signal-to-noise ratio （SNR） *** of single particle analysis has difficulty finding the correct orientation of particles on low SNR images,which results in the inability to restore the correct signal no matter how much data is superimposed,thus limiting resolving small *** possible way to enhance the SNR of small proteins is to reduce the acceleration voltage of *** lower the voltage,the greater proteins to scatter electrons,which can lead to more ***,lowering the voltage also introduces stronger radiation damage,