tRNA-based signature derived from salivary exosomes:tumor-growth modulator and prognostic biomarker in human esophageal carcinoma
作者单位：Institute of Precision Cancer medicine and Pathology Jinan University Medical College
会议名称：《2021年中国肿瘤标志物学术大会暨第十五届肿瘤标志物青...》
会议日期：2021年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
关 键 词：transfer RNA transfer RNA-derived small RNA salivary extracellular vesicles liquid biopsy
摘 要：Background: Transfer RNA-derived small RNAs（tsRNAs） is emerging as cancer signature with translational potential. Esophageal squamous cell carcinoma（ESCC） is ranked seventh for cancer morbidity and sixth for cancer mortality worldwide. Patients often present at an advantage stage with lymph node metastasis at the time of diagnosis, which leads to a 5-year survival rate of approximately20%. To discover cancer-specific tsRNAs with diagnostic and prognostic potentials for ESCC, we performed RNA sequencing of salivary exosomes from patients with ESCC, and evaluated the discovered tsRNAs for their potent function in cancer pathogenesis and their potentials as liquidbiopsy biomarker in human ***: Small-RNA transcriptome sequencing was performed using salivary exosomal RNA collected from ESCC patients vs. healthy controls. The top changed tsRNAs were validated and screened using qRT-PCR in salivary exosomes and tissues specimens of a discovery cohort of ESCC patients, as well as in relevant ESCC cell lines. The in vitro and in vivo models were used to examine the potent functions of tsRNAs in ESCC pathogenesis. Large cohorts of ESCC patients were further measured by RT-qPCR and analyzed for diagnosis, assessment of treatment response, and prediction of ***: RNA sequencing and validation studies demonstrated that two tsRNAs were specifically enriched in salivary exosomes and tissues of ESCC patients, as well as in human ESCC cell lines. In vitro and in vivo experiments showed that two tsRNAs exhibit malignant promoting capacities. We evaluated the diagnostic potentials by combining the two tsRNAs as a cancer signature in large cohorts of ESCC patients vs. healthy controls. Analysis of the data indicates that the tsRNA signature provides a reliable approach for diagnosis, treatment prediction and prognostic indicator for human ***: RNA sequencing of salivary exosomes following validation revealed a tsRNA signature for human ESCC. The tsRNAs