A DNA Nanotube-Peptide biocomplex for mRNA Detection and Its Application in Cancer Diagnosis and Targeted Therapy
作者单位：Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education College of Chemistry and Molecular Engineering Qingdao University of Science and Technology
会议名称：《中国化学会第十三届全国分析化学年会》
会议日期：2018年
学科分类：1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
关 键 词：DNA nanotube cRGD mRNA nanocarrier targeted release
摘 要：A biocomplex of DNA nanotube-peptide,consisting of six concatenated DNA strands,three lock DNA strands and a cell-penetrating peptide was developed *** MUC-1 aptamer which is part of DNA nanotube can specially recognize MUC-1 on the surface of MCF-7 *** c RGD facilitates recruitment and uptake of targeting drugs by binding to integrin receptors (αvβ3) of cytomembrane ***-cancer drug doxorubicin(DOX) and paclitaxel (PTX) were loaded into the capsulated DNA nanotube-peptide (CDNP),which was used as co-drug cargo *** as-prepared biocomplex can be utilized not only to deliver drug but also to achieve the anticancer effect in *** system that was composed by DNA strands and peptide has good biocompatibility and ***,the system can readily achieve detection of target mRNA of MCF-7 cell in *** detection limits of mRNA are 9.7×10 M and 1.8×10 M by using CDNP/DOX and CDNP/PTX-FITC as a probe,respectively.