Correlation analysis of lignin accumulation and expression of key genes involved in lignin biosynthesis of ramie(Boehmeria nivea)
作者单位：College of Life and Environmental Sciences Hunan University of Arts and Science College of Biological and Environmental Engineering Changsha University College of Agriculture Hunan Agricultural University
会议名称：《第十九届中国作物学会学术年会》
会议日期：2020年
关 键 词：ramie(Boehmeria nivea) lignin key gene expression correlation analysis enzyme activity assay
摘 要：The phloem of the stem of ramie(Boehmeria nivea) is an important source of natural fiber for the textile industry. However, the lignin content in the phloem affects the quality of ramie phloem fiber. In this study, the lignin content and related key gene expression levels were analyzed in the phloem and xylem at different developmental *** results showed that the relative expression levels of lignin synthesis-related key genes in the xylem and phloem of the stem gradually decreased from the fast-growing period to the late maturation period, but the corresponding lignin content increased significantly. However, the relative expression levels of a few genes were the highest during the maturation period. During all three periods, the lignin content in ramie stems was positively correlated with the expression of genes, including PAL, C4 H and 4 CL1 in the phenylpropanoid pathway, F5 H and CCoAOMT in the lignin-specific synthetic pathway, and CAD in the downstream pathway of lignin synthesis, but the lignin content was negatively correlated with the expression of genes including 4 CL3 in the phenylpropanoid pathway and UDP-GT in the shunt pathway of lignin monomer synthesis. The ramie 4 CL3 recombinant protein prefers cinnamic acid as a substrate during catalysis, and it negatively regulates lignin synthesis. It is speculated that ramie 4 CL3 is mainly involved in the synthesis of ramie flavonoid compounds, and that 4 CL1 is mainly involved in lignin synthesis.