A novel Fluorescent Protein Chromophore Analogue to simultaneously probe lysosome viscosity and β-Amyloid fibrils
作者单位：School of Biology and Biological EngineeringSouth China University of Technology
会议名称：《第十一届全国化学生物学学术会议》
会议日期：2019年
学科分类：081704[工学-应用化学] 1002[医学-临床医学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 1010[医学-医学技术（可授医学、理学学位）] 070302[理学-分析化学] 100203[医学-老年医学] 0703[理学-化学] 10[医学]
关 键 词：Alzheimer’s Disease Aβ fibrils lysosomal viscosity dual-functional fluorescent probe FP chromophore
摘 要：Alzheimer’s disease（AD） is an incurable neurodegenerative disease. To improve the diagnostic level of AD is essential to early treatment and effectively utilizes disease-modifying drugs. The amyloid cascade hypothesis and its revised version identify insoluble β-amyloid deposition as a good diagnostic biomarker for AD. We hypothesized that lysosomal viscosity may be an auxiliary biomarker for AD based on literature research. Herein, we have successfully developed the FP chromophore analogue C25 as a dual-functional fluorescent probe which respond to Aβ fibrils and viscosity in lysosome with distinguishable turn-on fluorescence signals around 570 nm and 600 nm, respectively. The probe C25 can also monitor the process of digesting Aβ in lysosome. The probe C25 can serve as a promising tool for developing the early diagnosis and innovative therapeutics of AD.