Preparation of β-glucan Oligosaccharides derived from Curdlan
作者单位：Key Laboratory of Marine DrugsMinistry of EducationSchool of Medicine and PharmacyOcean University of China Laboratory for Marine Drugs and Bioproducts of Pilot National Laboratory for Marine Science and Technology(Qingdao)
会议名称：《中国化学会第三届全国糖化学会议》
会议日期：2019年
学科分类：1007[医学-药学(可授医学、理学学位)] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070305[理学-高分子化学与物理] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 10[医学]
摘 要：β-glucans are commonly used as functional food ingredients and recognized as potential immunological ***,the BG’s poor water solubility of β-glucans limits their *** order to gain different molecular weight water-soluble β-glucans,we used chemical and physical methods to degrade the β-glucan of Curdlan.P4 fine gel exclusion column was used to separate the degradation product of *** β-glucans oligosaccharides(BGOs)derived from Curdlan were analyzed by HILIC-ELSD method with a Click Xion column,and then separated by graphitized carbon black *** with a specific degree of polymerization(DP) from 2 to 10 were ***-MS has indicated the BGOs(DP 2-10)molecular weight:342,504,666,828,990,1152,1314,1476 and *** anomeric configuration and glycosidic linkage of BGOs were confirmed to be β-Glc-(1→3)-by 1 H and 13 C NMR *** this study we developed a HILIC-ELSD method for the compositional analysis of BGOs and obtained BGOs with specific *** work could potentially provide a basis for further research on the structure-activity relationship of series oligosaccharides(DP2-9) from Curdlan.