Transcriptomic profiling of bovine mammary epithelial cells exposure to Staphylococcus aureus
作者单位：State Key Laboratory of Reproductive Regulation & Breeding of Grassland LivestockSchool of Life SciencesInner Mongolia University
会议名称：《中国生物化学与分子生物学会2020全国学术在线会议》
会议日期：2020年
学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学]
关 键 词：RNA-seq S.aureus BEMCs PI3K/Akt signaling
摘 要：Staphylococcus aureus(***) is an important pathogen causing subclinical mastitis of dairy *** of *** by non-professional phagocytic cells is a major suspected cause of persistent and severe *** performed comparative transcriptome profiling on bovine mammary epithelial cells(BMECs) that were infected with *** and untreated cells.A total of 608 differentially expressed genes(DEGs) appeared between untreated and *** infected *** on our quantitative transcriptomic analysis,60 significant DEGs with important function were related to cell adhesion,endocytosis,Staphylococcus aureus infection,Bacterial invasion of epithelial cells and PI3 K/Akt *** validate the translational significance of the findings in certain differentially expressed genes,PI3 K/Akt signaling were detected by western blot,and these two proteins showed a differential expression between nontreated and treated with *** *** induces transcriptomic changes,and PI3 K/Akt signaling might have significant function in bacteria invasion in *** RNA-seq data provides a valuable resource for the systematical profiling of *** infected on BMECs.