Dual-mode Detection of Bacterial Pathogens by ThermallyActivatedDelayed Fluorescence Probe
作者单位：State Key Laboratory of Bioactive Substances and Function of Natural MedicinesBeijing Key Laboratory of Active Substances Discovery and Drugability EvaluationInstitute of Materia MedicaChinese Academy of Medical Sciences ＆ Peking Union Medical College
会议名称：《第十一届全国化学生物学学术会议》
会议日期：2019年
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 1010[医学-医学技术（可授医学、理学学位）] 070302[理学-分析化学] 0703[理学-化学] 10[医学]
关 键 词：Dual-mode detection bacterial pathogens ribosomal RNA thermally activated delayed fluorescence time-resolved fluorescence imaging
摘 要：With the accumulation of bacterial resistance to antibiotics, bacterial infection has become a global crisis. Specific detection of pathogens has long been recognized as a vital strategy in the control of bacterial infections.[1] Herein, a novel hydrophilic aromatic-imide-based[2] thermally activated delayed fluorescence（TADF） probe, AI-Cz-Neo, is rationally designed and efficiently synthesized by conjugating a broad spectrum aminoglycoside antibiotic,[3] neomycin. AI-Cz-Neo shows specific targetability to bacterial ribosomal RNA, which provides not only high specificity for bacteria but also a practical strategy for long-lived delayed fluorescence emission in oxygenic environments. Notably, AI-Cz-Neo was successfully utilized for the specific detection of broad-spectrum bacterial pathogens with high reliability and accuracy on the basis of the dual-mode imaging of confocal fluorescence imaging and fluorescence lifetime imaging in cells and tissues. To our best knowledge, AI-Cz-Neo is the first molecule providing the dual-mode fluorescence responses in detection of bacterial pathogens, which will arouse more attention to discover more molecular probes for bacterial imaging and diagnosis. Furthermore, this study greatly expanded the applications of TADF fluorophores in time-resolved biological imaging.