A gold-selenium-bonded nanoprobe for real-time in situ imaging of the upstream and downstream relationship between uPA and MMP-9 in cancer cells
作者单位：College of ChemistryChemical Engineering and Materials ScienceKey Laboratory of Molecular and Nano ProbesMinistry of EducationShandong Normal University School of Chemistry and Pharmaceutical EngineeringQilu University of Technology (Shandong Academy of Sciences)
会议名称：《第十一届全国化学生物学学术会议》
会议日期：2019年
学科分类：081704[工学-应用化学] 07[理学] 070205[理学-凝聚态物理] 08[工学] 070302[理学-分析化学] 1002[医学-临床医学] 0817[工学-化学工程与技术] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 100214[医学-肿瘤学] 0703[理学-化学] 10[医学] 0702[理学-物理学]
关 键 词：uPA MMP-9 Au-Se nanoprobe regulatory relationships
摘 要：Tumour progression is closely related to the abnormal activation of signalling pathways involving uPA-mediated MMP-9 activation. The study of protein expression levels and their relationships will promote an understanding of the role of regulatory mechanisms in cancer development.[1] Herein, a novel Au-Se nanoprobe with a remarkable anti-interference ability for glutathione was developed to real-time in situ monitor uPA and MMP-9 proteins. The imaging results indicate that the Au-Se nanoprobe can observe the sequential expression of uPA and MMP-9 in situ, and identify that upstream uPA can activate downstream MMP-9. The Au-Se nanoprobe possesses better prevention of GSH interference and can be applied in complicated biological environments compared to the conventional Au-S nanoprobe. The nanoprobe offers avenues for studying other signalling molecules and regulatory relationships in the pathway. [2]