Enzymatic synthesis of isoquercitrin with naringinase under microwave irradiation
作者单位：江苏科技大学生物技术学院 School of BiotechnologyJiangsu University of Science and Technology Sericultural Research InstituteChinese Academy of Agricultural Sciences
会议名称：《中国生物工程学会第六次全国会员代表大会暨第九届学术年会》
会议日期：2015年
学科分类：08[工学] 082203[工学-发酵工程] 0822[工学-轻工技术与工程]
关 键 词：Microwave irradiation Naringinase Isoquercitrin Enzymatic synthesis
摘 要：Isoquercitrin,a key synthetic intermediate,has a wide range of biological activities,for the production of enzymatically modified isoquercitrin(EMIQ) which has received FDA safety ***,the synthesis of isoquercitrin is still problematic,requires a long reaction time,is unstable and easily oxidizes *** order to establish an efficient bioprocess for obtaining isoquercitrin,a biosynthesis of isoquercitrin using the naringinase-catalyzed hydrothermal synthesis of rutin under microwave irradiation was first carried *** has been proved the feasibility of the free enzyme under microwave *** irradiation can effectively strengthen the enzymatic reaction,reduce the activation *** also can change the enzyme microscopic configuration and the reaction *** catalytic effect has a great thrust on the promotion of the enzymatic reactions in industrial *** micro-technology may be applied to the effective synthesis of other precious ***,a bright future and a better effect would be achieved by unremitting *** this study,enzymatic reactions were performed in a microwave reactor for isoquercitrin *** volume ratio of enzyme solution,ionic liquid and rutin dissolved by buffer solution in the enzymatic synthesis of isoquercitrin reaction system was 180:720:*** reaction was initiated by adding naringinase and the mixtures at various substrate concentrations,enzyme concentrations,reaction time,microwave power and types of ionic *** microwave irradiation,the mixture sampled for every 10 min from the 30 min to the 70 *** crude hydrolysis products of rutin were determined and verificated by HPLC and *** yield was calculated as follows Eq.(1).Isoquercitrin yield(%)=moles of isoquercitrin/initial moles of rutin×100% (1)Reaction medium directly affects the reaction rate and the enzyme ***,ionic liquid has the strong ability of absorbing microwave,a