Synthesis,Biophysical and Biochemical Studies of C4’-OCH3 Modified Ribonucleotides
作者单位：State Key of Laboratory of Element-organic Chemistrycollege of ChemistryNankai University
会议名称：《第十一届全国化学生物学学术会议》
会议日期：2019年
学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学]
关 键 词：ribonucleotide ribose confirmation chemical modifications
摘 要：C4′-modified ribonucleotides have recently attracted great interests.[1] C4′ modifications introduce stereoelectronic effect and thus modulate the conformation of the ribose, without losing the 2′-OH. For instance, we have synthesized C4′-Me modified rU（C4′-Me-rU） and found it is constrained in an unusual South conformation.[2, 3] Methoxy group generally render different electronic effects than methyl group. Herein, we synthesized 4’-OCH3-rU and introduced it into oligonucleotides based on solid phase synthesis（Figure 1）. The structure of 4’-OCH3-rU was studied by NMR, and the biophysical and biochemical properties of these modified oligonucleotides were examined.