Design,Synthesis,and Anticancer Effect Studies of Iridium(Ⅲ) Polypyridyl Complexes against HeLa Cells
作者单位：School of PharmacyGuangdong Pharmaceutical University
会议名称：《第十一届全国化学生物学学术会议》
会议日期：2019年
学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学] 100701[医学-药物化学]
关 键 词：Iridium complex antitumor activity mitochondrial dysfunction DNA damage
摘 要：An iridium(Ⅲ) complex([Ir(PTTP)2(ppy)](PF)(Ir-1) were synthesized and characterized. The cytotoxicity of Ir-1 against cancer cell lines SGC-7901, A549, HeLa, HepG2 and BEL-7402 was investigated using the MTT method. The results showed that the iridium(Ⅲ) complex had moderate in vitro anti-tumor activity toward HeLa cells. Further studies showed that the complex induce cell apoptosis through DNA damage, cell cycle arrest at the G0/G1 phase, ROS elevation, increased levels of Ca, high mitochondrial membrane depolarization, and cellular ATP depletion. Transwell and Colony-Forming assays revealed that Ir-1 can also effectively inhibit the metastasis and proliferation of tumor cells. These results demonstrate that Ir-1 induce apoptosis in HeLa cells through ROS-mediated mitochondrial damage and DNA damage pathways, as well as by inhibiting cell invasion, thereby exerting anti-tumor cell proliferation activity in vitro.