Development and application of a liquid chromatographic method for analysis of adenosine in royal jelly
作者单位：Research InstituteChinese Academy of Agricultural Sciences Bee College of Animal SciencesZhejiang University
会议名称：《二十一世纪第二届全国蜂业科技与蜂产业发展大会》
会议日期：2016年
学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学]
关 键 词：Adenosine Royal jelly HPLC
摘 要：A simple method is described for the determination of adenosine in royal *** adenosine in the sample was extracted using 80% ethanol and analyzed by reversed-phase liquid *** separation was performed using a Dionex HPLC system with a Waters Symmetry C18 column,and with 0.4% phosphoric acid and methanol gradient *** average recoveries were 91.6% to 98.3%（n=6）with standard deviation below 5.3%.The limits of detection and quantification were 1.2 mg/kg and 4.1 mg/kg,*** developed method has been successfully applied to analyze royal jelly *** 45 royal jelly products,the adenosine content varied from 5.9 to 2057.4 mg/kg.