The inhibition role of miR-485-5p in colorectal cancer proliferation and invasion via targeting CD147
作者单位：Central LaboratoryNanjing First HospitalNanjing Medical University
会议名称：《2017年中国肿瘤标志物学术大会暨第十一届肿瘤标志物青年科学家论坛》
会议日期：2017年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
关 键 词：MiR-485-5p Tumor-suppressing Colorectal cancer CD147 Pathogenesis
摘 要：Objective: The present study was designed to explore the expression and biological role of miR-485-5 p in the colorectal cancer(CRC). Methods: The expression of miR-485-5 p in forty-seven pairs of CRC and adjacent non-tumor tissues and CRC cell lines were detected by quantitative RT-PCR. CCK8 proliferation assay and invasion assay were performed to test the proliferation and invasion of CRC cells after overexpression of miR-485-5 p. The effect of miR-485-5 p on CRC in vivo was validated by murine xenograft model. The relationship of miR-485-5 p and its target gene CD147 was also investigated. Results: We found that the expression of miR-485-5 p in CRC tissues and cell lines were much lower than that in control, respectively. Moreover, the overexpression of miR-485-5 p could inhibit the proliferation and invasion of CRC cell lines in vitro and in vivo. Furthermore, we found that miR-485-5 p could decrease the expression of CD147 by directly targeting CD147 3’UTR. In addition, the expression of CD147 was inversely correlated with the expression of miR-485-5 p in CRC tissues. Conclusions: Our results suggested that miR-485-5 p play a pivotal role of tumor-suppressing in CRC through down-regulating cancer-associated gene CD147, which may provide a better understanding of the pathogenesis in CRC and a new bio-target for CRC therapy.