The effect of bufalin in human esophageal squamous cell carcinoma cells and xenograft model of nude mice
作者单位：Department of PathologyFourth Hospital of Hebei Medical University Department of RadiologyChinese people's Liberation Army 252nd hospital
会议名称：《2017年中国肿瘤标志物学术大会暨第十一届肿瘤标志物青年科学家论坛》
会议日期：2017年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
关 键 词：Esophageal carcinoma Bufalin ERK/RSK2 signaling pathway FAK
摘 要：Background: Bufalin exhibits significant antineoplastic activities in many neoplasms. The aim of this study was to evaluate the effects of Bufalin on ERK/RSK2 pathway and EMT and their mechanisms of action in human esophageal squamous cell carcinoma cells(ECA109 cells). Methods: ECA109 cells were incubated with bufalin, TUNEL, Transwell migration/invasion assay, PT-PCR and Western blotting were performed in ECA109 cell lines. Established the ESCC transplantation neoplasm in nude mice and treated with Bufalin. The mRNA levels(ERK, RSK2, E-Cadherin and Vimentin) and the protein levels(ERK1/2, pERK1/2, RSK2, pRSK2, BAD, pBAD and Fascin-1) were detected by Real-time quantitative PCR and Western blot analysis in nude mice transplanted neoplasm. Results: Bufalin inhibited ECA109 cell migration, invasion and promote apoptosis. Treatment with Bufalin significantly decreased relative expression of ERK, RSK2 and FAK m RNA in nude mice *** protein levels of pERK, pRSK2, pBAD and Fascin-1 was ***: Bufalin can exert an inhibitory effect on esophageal cancer ECA109 cells and xenografts in nude mice through the ERK/RSK2 pathway and also significantly inhibit FAK and the process of EMT in esophageal cancer.