Simultaneous quantification of twenty-one ginsenosides and their three aglycones in rat plasma by a developed UFLC-MS/MS assay:Application to a pharmacokinetic study of red ginseng
作者单位：State Key Laboratory of Natural and Biomimetic Drugs and Department of Natural MedicinesSchool of Pharmaceutical SciencesPeking University
会议名称：《第三届全国药物代谢动力学青年科学家论坛暨刘昌孝人才奖颁奖大会》
会议日期：2017年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
摘 要：To track the pharmacokinetic features of red ginseng(RG),a rapid and sensitive ultra fast liquid chromatographic coupled with electrospray ionization triple quadrupole tandem mass spectrometry(UFLC-MS/MS) method was developed for simultaneous quantification of twenty-one ginsenosides and their three aglycones,including 18 prototype compounds(ginsenosides Rb1,Rb2,Rc,Rd,Re,Rg1,Rg5,Rh4,Rk1,Rk3,20(S)-Rf,20(S)-Rg2,20(R)-Rg2,20(S)-Rg3,20(R)-Rg3,20(S)-Rh1,20(R)-Rh1,20(S)-NG-R2),and 6 metabolites(ginsenosides 20(S)-Rh2 and Rh3,20(S)-protopanaxadiol(PPD),20(S)-protopanaxatriol(PPT),20(R)-PPT,ginseng saponin compound K) of RG in rat plasma after oral administration of RG water extract at a single dose of 4 g/kg body weight to *** analytes with internal standard(digoxin) were detected by multiple reaction monitoring in negative ionization mode and separated on an ACQUITY UPLC? BEH RP-C18 column(1.7 μm,100×2.1 mm).This established method was well validated in terms of linearity,sensitivity,intra-and inter-day precisions,accuracy,recovery,matrix effect,stability,and had a lower limit of quantification at the concentration range of 0.12-8.12 ng/mL for all of *** UFLC-MS/MS approach was successfully applied to the pharmacokinetic study for RG water extract in rats,and further with a method improvement,to evaluate the permeabilities of 40 ginsenosides and their two aglycones of RG using human intestinal Caco-2 cell monolayer *** firstly proposed that Rbl,Rb2,Rc,Rd,Rg1,Rg5,20(S)-Rg3,20(S)-Rh2,and 20(S)-PPD measured in rat plasma were suitable pharmacokinetic markers of RG extract in rats due to their high systemic exposure ***,this specific and reliable method will be useful for future applications to pharmacokinetic studies for various sources of ginsenoside samples and Panax herbs in ***:Red ginseng,Ginsenoside,UFLC-MS/MS,Pharmacokinetics,human intestinal Caco-2 cell monolayer,Rat plasma